safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
7	1	0	7.07

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
ADDNNMEL_1	2828953	2829765	ErmF	ARO:3000498	ErmF	99.62	101.50	M17124.1
ADDNNMEL_1	2827604	2828740	tet(X)	ARO:3000205	tet(X)	99.74	97.42	M37699.1
ADDNNMEL_1	2823778	2825703	tet(Q)	ARO:3000191	tet(Q)	93.92	97.56	Z21523.1
ADDNNMEL_1	1602435	1603640	Mef(En2)	ARO:3004659	Mef(En2)	99.50	100.00	AF251288.1
ADDNNMEL_1	1579310	1580212	cepA	ARO:3003559	cepA	100.00	100.00	U05883.1
ADDNNMEL_1	228096	230021	tet(Q)	ARO:3000191	tet(Q)	97.97	97.56	Z21523.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
ADDNNMEL_1	2828953	2829753	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	100.00	100.00	M17808
ADDNNMEL_1	2827574	2828740	tet(X)	Doxycycline, Tetracycline, Minocycline, Tigecycline	99.91	100.00	M37699
ADDNNMEL_1	1579310	1580212	cepA	Unknown Beta-lactam	99.67	100.00	L13472
ADDNNMEL_1	228096	230021	tet(Q)	Doxycycline, Tetracycline, Minocycline	97.77	100.00	L33696

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
ADDNNMEL_1	1579313	1580212	cepA	CepA family extended-spectrum class A beta-lactamase	EXACTX	100.00	100.00	WP_005795628.1
ADDNNMEL_1	2828956	2829753	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	EXACTX	100.00	100.00	WP_002682030.1
ADDNNMEL_1	1603665	1604174	lnu(AN2)	lincosamide nucleotidyltransferase Lnu(AN2)	EXACTX	100.00	100.00	WP_004308783.1
ADDNNMEL_1	2827574	2828737	tet(X2)	tetracycline-inactivating monooxygenase Tet(X2)	BLASTX	99.74	100.00	WP_008651082.1
ADDNNMEL_1	1602435	1603637	mef(En2)	macrolide efflux MFS transporter Mef(En2)	BLASTX	99.50	100.00	WP_063853729.1
ADDNNMEL_1	228096	230018	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	97.97	100.00	WP_002560998.1
ADDNNMEL_1	2823778	2825700	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	95.63	100.00	WP_063856407.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
ADDNNMEL_4	71484	72677	bft-2	BFT B. fragilis toxin, subtype 2	Exotoxin	100.00	100.00	BAA77277
ADDNNMEL_4	71484	72677	bft-3	BFT B. fragilis toxin, subtype 3	Exotoxin	96.90	100.00	BAA77275
ADDNNMEL_4	71484	72677	bft-1	BFT B. fragilis toxin, subtype 1	Exotoxin	94.89	100.00	BAA77276

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
ADDNNMEL_1	0.0	1.0	0.0
ADDNNMEL_2	0.0	1.0	0.0
ADDNNMEL_3	0.0	1.0	0.0
ADDNNMEL_4	0.0	1.0	0.0
ADDNNMEL_5	0.0	1.0	0.0
ADDNNMEL_6	0.0	1.0	0.0
ADDNNMEL_7	1.0	0.0	1.0
ADDNNMEL_8	1.0	0.0	1.0
ADDNNMEL_9	1.0	0.0	1.0
ADDNNMEL_10	1.0	0.0	1.0
ADDNNMEL_11	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
ADDNNMEL_1	3091486	3097349	False	Siphoviridae	VOG7238,
ADDNNMEL_2	554407	566398	False	Unknown	VOG4572,

Phigaro v2.4.0 (Database: Jan. 2024 release)