Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 16 | 32 | 35.78 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HLOKCMLO_91 | 13850 | 14443 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| HLOKCMLO_91 | 13850 | 14443 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| HLOKCMLO_91 | 13850 | 14443 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| HLOKCMLO_74 | 799 | 2457 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| HLOKCMLO_91 | 14443 | 15312 | rfbA | 0.0 | Capsule | Immune modulation | 99.19 | 100.00 | WP_011681286 |
| HLOKCMLO_91 | 49211 | 49972 | srtA | 0.0 | Sortase A | Exoenzyme | 98.42 | 100.00 | - |
| HLOKCMLO_90 | 18760 | 19554 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.11 | 100.25 | - |
| HLOKCMLO_85 | 2695 | 3426 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.95 | 100.00 | WP_011681176 |
| HLOKCMLO_36 | 6628 | 7590 | cbpD | 0.0 | Autolysin | Exoenzyme | 97.73 | 99.90 | WP_082302295 |
| HLOKCMLO_85 | 1244 | 1984 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.71 | 100.00 | WP_011226046 |
| HLOKCMLO_85 | 2695 | 3426 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.68 | 100.00 | WP_011226048 |
| HLOKCMLO_85 | 2695 | 3426 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.27 | 100.00 | WP_011227248 |
| HLOKCMLO_111 | 7 | 858 | rfbD | 0.0 | Capsule | Immune modulation | 97.07 | 100.00 | WP_014621820 |
| HLOKCMLO_85 | 1244 | 1984 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.03 | 100.00 | WP_011681174 |
| HLOKCMLO_85 | 3427 | 4887 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 96.65 | 100.00 | WP_011681177 |
| HLOKCMLO_85 | 1249 | 1984 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.47 | 98.13 | WP_011227246 |
| HLOKCMLO_85 | 3427 | 4887 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.44 | 100.21 | WP_011226049 |
| HLOKCMLO_85 | 1994 | 2686 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 96.10 | 100.00 | WP_011227247 |
| HLOKCMLO_85 | 1994 | 2686 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 96.10 | 100.00 | WP_011226047 |
| HLOKCMLO_85 | 3427 | 4887 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.76 | 100.21 | WP_041827043 |
| HLOKCMLO_85 | 1 | 1187 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.52 | 86.77 | WP_011226045 |
| HLOKCMLO_85 | 1 | 1187 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.36 | 86.77 | WP_011681173 |
| HLOKCMLO_85 | 1994 | 2686 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.75 | 100.00 | WP_011681175 |
| HLOKCMLO_45 | 47041 | 48344 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| HLOKCMLO_129 | 631 | 1641 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.69 | 99.70 | WP_002986042 |
| HLOKCMLO_91 | 12375 | 13421 | rfbB | 0.0 | Capsule | Immune modulation | 80.71 | 100.00 | WP_009659423 |
| HLOKCMLO_16 | 5241 | 6524 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.50 | 99.77 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HLOKCMLO_91 | 52659 | 53642 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.51 | 100.00 | AAK75326 |
| HLOKCMLO_56 | 24273 | 25346 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| HLOKCMLO_123 | 35442 | 37745 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| HLOKCMLO_42 | 1881 | 2849 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| HLOKCMLO_102 | 4332 | 5813 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.51 | 100.00 | ABJ54480 |
| HLOKCMLO_57 | 5612 | 7858 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| HLOKCMLO_6 | 12168 | 13160 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| HLOKCMLO_3 | 4132 | 5103 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| HLOKCMLO_116 | 16990 | 15839 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| HLOKCMLO_114 | 10735 | 8747 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| HLOKCMLO_91 | 36461 | 34731 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| HLOKCMLO_142 | 25796 | 24318 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| HLOKCMLO_45 | 47041 | 48342 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| HLOKCMLO_142 | 11059 | 9167 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| HLOKCMLO_57 | 10783 | 9074 | PGM | 0.0 | phosphoglucomutase | 83.36 | 99.65 | ACH87041 |
| HLOKCMLO_131 | 21509 | 22519 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| HLOKCMLO_76 | 2630 | 1269 | mnmE | 0.0 | central tRNA-modifying GTPase | 81.80 | 99.56 | ABP90420 |
| HLOKCMLO_133 | 4816 | 2924 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| HLOKCMLO_45 | 32603 | 33493 | bglB | 4.47e-180 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| HLOKCMLO_64 | 3890 | 2955 | SP_0676 | 4.44e-170 | transcription facot | 81.73 | 96.89 | AAK74821 |
| HLOKCMLO_57 | 8050 | 8907 | metF | 7.61e-167 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| HLOKCMLO_88 | 24434 | 23730 | covR | 5.85e-133 | responder protein | 85.53 | 100.00 | AAK71319 |
| HLOKCMLO_96 | 3521 | 2850 | rr01 | 7.42e-131 | response regulator | 84.38 | 99.56 | CAB54566 |
| HLOKCMLO_88 | 24434 | 23730 | walR | 7.87e-123 | DNA-binding response regulator | 81.28 | 100.00 | ANC99504 |
| HLOKCMLO_26 | 6787 | 7473 | CovR | 6.91e-120 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| HLOKCMLO_6 | 13457 | 13852 | SpxA2 | 2.84e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| HLOKCMLO_76 | 13987 | 14388 | SpxA1 | 4.88e-68 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| HLOKCMLO_29 | 13031 | 13318 | spr0479 | 1.43e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| HLOKCMLO_37 | 33471 | 33725 | spr1327 | 7.14e-46 | essential gene | 95.29 | 100.00 | AAL00131 |
| HLOKCMLO_138 | 2361 | 2624 | spr0175 | 3.78e-43 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| HLOKCMLO_138 | 3164 | 3454 | spr0177 | 2.21e-38 | essential gene | 81.00 | 98.02 | AAK98981 |
| HLOKCMLO_16 | 6682 | 7182 | RopE (M5005_Spy_1611) | 9.94e-77 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| HLOKCMLO_123 | 35442 | 37739 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.68 | 98.08 | MYN69560 |
| HLOKCMLO_132 | 33890 | 32871 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HLOKCMLO_1 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_3 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_4 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_5 | 1.0 | 0.0 | 1.0 |
| HLOKCMLO_6 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_7 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_8 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_9 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_11 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_16 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_17 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_18 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_19 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_20 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_21 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_22 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_23 | 1.0 | 0.0 | 1.0 |
| HLOKCMLO_25 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_26 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_27 | 1.0 | 0.0 | 1.0 |
| HLOKCMLO_29 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_30 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_31 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_32 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_33 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_34 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_35 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_36 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_37 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_38 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_39 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_40 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_41 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_42 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_43 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_44 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_45 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_47 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_48 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_49 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_50 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_52 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_54 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_55 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_56 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_57 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_58 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_59 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_60 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_61 | 1.0 | 0.0 | 1.0 |
| HLOKCMLO_62 | 1.0 | 0.0 | 1.0 |
| HLOKCMLO_63 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_64 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_65 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_66 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_67 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_68 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_69 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_71 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_72 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_73 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_74 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_75 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_76 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_77 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_78 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_80 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_84 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_85 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_86 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_88 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_90 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_91 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_92 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_96 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_99 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_100 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_101 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_102 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_103 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_104 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_105 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_107 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_108 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_109 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_110 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_114 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_116 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_119 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_120 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_121 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_122 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_123 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_124 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_126 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_127 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_128 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_129 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_130 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_131 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_132 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_133 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_134 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_135 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_137 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_138 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_139 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_140 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_142 | 0.0 | 1.0 | 0.0 |
| HLOKCMLO_143 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)