Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 1 | 2 | 8 | 8.31 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JEDNLBFD_5 | 67778 | 68827 | clbA | ARO:3002814 | clbA | 99.71 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| JEDNLBFD_5 | 67792 | 68827 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 89.09 | 98.67 | KR610408 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JEDNLBFD_5 | 67781 | 68827 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.71 | 100.00 | WP_012116915.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JEDNLBFD_9 | 139429 | 140610 | capB | 0.0 | Capsule | Immune modulation | 81.96 | 99.66 | NP_391471 |
| JEDNLBFD_9 | 140625 | 141075 | capC | 2.92e-113 | Capsule | Immune modulation | 83.41 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JEDNLBFD_8 | 201360 | 202496 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| JEDNLBFD_21 | 12436 | 11567 | pdxS | 1.36e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| JEDNLBFD_1 | 333043 | 332267 | CodY | 5.41e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| JEDNLBFD_2 | 201833 | 201441 | spxA1 | 8.69e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| JEDNLBFD_5 | 84889 | 84692 | CspA | 6.95e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| JEDNLBFD_19 | 37759 | 37565 | CspB | 1.09e-27 | cold shock protein | 84.61 | 98.48 | CAD00094 |
| JEDNLBFD_3 | 166276 | 166470 | CspD | 1.39e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| JEDNLBFD_5 | 84889 | 84695 | CspR | 9.10e-27 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| JEDNLBFD_8 | 26713 | 27585 | lipA (SAUSA300_0829) | 1.35e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JEDNLBFD_1 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_2 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_3 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_4 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_5 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_6 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_7 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_8 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_9 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_10 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_11 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_12 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_13 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_14 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_15 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_16 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_17 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_18 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_19 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_20 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_21 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_22 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_23 | 0.0 | 1.0 | 0.0 |
| JEDNLBFD_24 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JEDNLBFD_2 | 90322 | 116425 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG5660, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
| JEDNLBFD_11 | 15562 | 45016 | False | Siphoviridae | VOG0136, VOG5221, VOG4705, VOG0804, VOG4856, VOG4619, VOG3462, VOG0801, VOG6163, VOG5852, VOG4586, VOG0704, VOG4589, VOG0204, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841, VOG7958, VOG2545, VOG0376, VOG5447, VOG2981, VOG0425, VOG1884, VOG0025, VOG9600, VOG11060, VOG3547, VOG4552 |
Phigaro v2.4.0 (Database: Jan. 2024 release)