safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	29	38.29

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
MFMAKCMH_1	1313100	1313693	STER_RS06030	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
MFMAKCMH_1	1186781	1188241	STER_RS05320	0.0	Capsule	Immune modulation	99.93	100.00	WP_011681177
MFMAKCMH_1	1313100	1313693	STR_RS05880	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
MFMAKCMH_1	1525744	1526553	STU_RS16245	0.0	Capsule	Immune modulation	99.75	100.00	WP_002948020
MFMAKCMH_1	1313693	1314562	rfbA	0.0	Capsule	Immune modulation	99.66	100.00	WP_011681286
MFMAKCMH_1	1313100	1313693	STU_RS15260	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
MFMAKCMH_1	1068144	1069802	fbp54	0.0	FBPs	Adherence	99.64	100.00	WP_011227179
MFMAKCMH_1	1526537	1527493	STU_RS16250	0.0	Capsule	Immune modulation	99.58	100.00	WP_011226328
MFMAKCMH_1	233715	234554	cbpD	0.0	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
MFMAKCMH_1	1521671	1523455	STU_RS16230	0.0	Capsule	Immune modulation	99.44	100.00	WP_011226325
MFMAKCMH_1	1524539	1525744	STU_RS16240	0.0	Capsule	Immune modulation	99.17	100.00	WP_011226326
MFMAKCMH_1	1533680	1534531	rfbD	0.0	Capsule	Immune modulation	99.06	100.00	WP_014621820
MFMAKCMH_1	1186049	1186780	STER_RS05315	0.0	Capsule	Immune modulation	98.91	100.00	WP_011681176
MFMAKCMH_1	1349886	1350647	srtA	0.0	Sortase A	Exoenzyme	98.82	100.00	-
MFMAKCMH_1	1527490	1528638	STU_RS16255	0.0	Capsule	Immune modulation	98.78	100.00	WP_041828290
MFMAKCMH_1	1186049	1186780	STR_RS05260	0.0	Capsule	Immune modulation	98.50	100.00	WP_011227248
MFMAKCMH_1	1186049	1186780	STU_RS14645	0.0	Capsule	Immune modulation	98.36	100.00	WP_011226048
MFMAKCMH_1	1254126	1254919	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	98.24	100.25	-
MFMAKCMH_1	1174620	1176035	STER_RS05250	0.0	Capsule	Immune modulation	98.23	100.00	WP_011681165
MFMAKCMH_1	1184598	1185338	STU_RS14635	0.0	Capsule	Immune modulation	98.11	100.00	WP_011226046
MFMAKCMH_1	1184598	1185338	STER_RS05305	0.0	Capsule	Immune modulation	97.71	100.00	WP_011681174
MFMAKCMH_1	1186781	1188241	STU_RS14650	0.0	Capsule	Immune modulation	97.40	100.21	WP_011226049
MFMAKCMH_1	1184603	1185338	STR_RS05250	0.0	Capsule	Immune modulation	97.15	98.13	WP_011227246
MFMAKCMH_1	1183174	1184541	STU_RS14630	0.0	Capsule	Immune modulation	97.00	100.00	WP_011226045
MFMAKCMH_1	1186781	1188241	STR_RS05265	0.0	Capsule	Immune modulation	96.85	100.21	WP_041827043
MFMAKCMH_1	1185348	1186040	STR_RS05255	0.0	Capsule	Immune modulation	95.38	100.00	WP_011227247
MFMAKCMH_1	1185348	1186040	STU_RS14640	0.0	Capsule	Immune modulation	95.38	100.00	WP_011226047
MFMAKCMH_1	1523452	1524519	STU_RS16235	0.0	Capsule	Immune modulation	95.27	101.14	WP_002948022
MFMAKCMH_1	1183176	1184541	STER_RS05300	0.0	Capsule	Immune modulation	94.58	99.85	WP_011681173
MFMAKCMH_1	1185348	1186040	STER_RS05310	0.0	Capsule	Immune modulation	90.48	100.00	WP_011681175
MFMAKCMH_1	1531303	1532577	STU_RS16265	0.0	Capsule	Immune modulation	89.42	99.92	WP_011226331
MFMAKCMH_1	787235	788538	eno	0.0	Streptococcal enolase	Exoenzyme	88.02	99.46	WP_000022832
MFMAKCMH_1	1820066	1821076	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
MFMAKCMH_1	329945	331228	tig/ropA	0.0	Trigger factor	Stress survival	80.73	99.77	WP_000107753
MFMAKCMH_1	1311625	1312671	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
MFMAKCMH_1	1172217	1172800	SPD_RS01775	1.08e-151	Capsule	Immune modulation	83.59	96.20	WP_001819836

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
MFMAKCMH_1	1353334	1354317	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
MFMAKCMH_1	906504	907577	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
MFMAKCMH_1	1697087	1699390	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
MFMAKCMH_1	703600	704568	spr0084	0.0	pathogenitcity related	92.57	98.48	AAK98888
MFMAKCMH_1	934824	937070	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
MFMAKCMH_1	1423644	1425125	SPD_0310	0.0	UPF0371 protein SPD_0310	91.70	100.00	ABJ54480
MFMAKCMH_1	253345	254343	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
MFMAKCMH_1	217232	218203	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
MFMAKCMH_1	1583070	1581919	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
MFMAKCMH_1	1547708	1545720	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
MFMAKCMH_1	1337135	1335405	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
MFMAKCMH_1	179066	177588	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
MFMAKCMH_1	787235	788536	Eno	0.0	enolase	86.21	99.77	ABP90468
MFMAKCMH_1	163877	161985	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
MFMAKCMH_1	939995	938286	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
MFMAKCMH_1	17968	18978	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
MFMAKCMH_1	1091656	1090295	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
MFMAKCMH_1	68311	66419	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
MFMAKCMH_1	772799	773689	bglB	5.23e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
MFMAKCMH_1	1013046	1012108	SP_0676	2.35e-167	transcription facot	81.15	97.20	AAK74821
MFMAKCMH_1	937262	938119	metF	1.52e-164	methionine biosynthesis enzymes	86.36	99.31	AAK74739
MFMAKCMH_1	1226447	1225743	covR	4.36e-131	responder protein	85.53	100.00	AAK71319
MFMAKCMH_1	1404669	1403998	rr01	2.99e-124	response regulator	83.93	99.56	CAB54566
MFMAKCMH_1	1226447	1225743	walR	1.84e-121	DNA-binding response regulator	82.13	100.00	ANC99504
MFMAKCMH_1	482966	483652	CovR	1.00e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
MFMAKCMH_1	943949	944617	CiaR	2.01e-117	two-component response regulator	84.31	99.55	AAK74935
MFMAKCMH_1	943949	944620	ciaR	1.98e-115	two component system response regulator	82.14	99.12	AIK75708
MFMAKCMH_1	254619	254993	SpxA2	1.15e-69	transcriptional regulator	91.20	94.70	ERL21461
MFMAKCMH_1	1103018	1103419	SpxA1	3.75e-66	transcriptional regulator	80.60	100.00	ERL19273
MFMAKCMH_1	331386	331886	RopE (M5005_Spy_1611)	4.90e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
MFMAKCMH_1	1697087	1699384	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
MFMAKCMH_1	53852	52833	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
MFMAKCMH_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found