safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
KIIMKAGJ_1	2708448	2709302	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
KIIMKAGJ_1	2689739	2690941	blt	ARO:3003006	blt	100.00	100.00	L32599.1
KIIMKAGJ_1	2467422	2468591	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
KIIMKAGJ_1	1360406	1360723	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
KIIMKAGJ_1	1360068	1360406	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
KIIMKAGJ_1	900326	902896	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
KIIMKAGJ_1	588283	589926	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
KIIMKAGJ_1	339164	339757	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
KIIMKAGJ_1	288661	290094	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
KIIMKAGJ_1	275846	276766	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
KIIMKAGJ_1	2708448	2709302	aadK	Streptomycin	100.00	100.00	M26879
KIIMKAGJ_1	275846	276766	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
KIIMKAGJ_1	4160444	4161820	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
KIIMKAGJ_1	2708451	2709302	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
KIIMKAGJ_1	275846	276763	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
KIIMKAGJ_1	4157926	4158444	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
KIIMKAGJ_1	4160447	4161820	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
KIIMKAGJ_1	588286	589926	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
KIIMKAGJ_1	2024103	2026697	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
KIIMKAGJ_1	3670588	3671730	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
KIIMKAGJ_1	3672213	3673394	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
KIIMKAGJ_1	3671749	3672198	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
KIIMKAGJ_1	1977445	1979208	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
KIIMKAGJ_1	3264278	3265063	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
KIIMKAGJ_1	3260442	3261380	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
KIIMKAGJ_1	3263056	3264252	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
KIIMKAGJ_1	3261408	3263027	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
KIIMKAGJ_1	3253286	3260422	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
KIIMKAGJ_1	2268070	2268711	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
KIIMKAGJ_1	132882	134018	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
KIIMKAGJ_1	19074	19943	pdxS	3.11e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
KIIMKAGJ_1	1673670	1674446	CodY	3.40e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
KIIMKAGJ_1	3519001	3519570	ClpP	2.52e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
KIIMKAGJ_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
KIIMKAGJ_1	1304121	1330229	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
KIIMKAGJ_1	2639811	2670055	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)