Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 7 | 16.43 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IMAMHKKF_9 | 598818 | 599672 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| IMAMHKKF_9 | 580043 | 581245 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| IMAMHKKF_6 | 485251 | 485568 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| IMAMHKKF_6 | 484913 | 485251 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| IMAMHKKF_9 | 387848 | 389017 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| IMAMHKKF_1 | 133083 | 133676 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| IMAMHKKF_1 | 82584 | 84023 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| IMAMHKKF_1 | 69772 | 70692 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| IMAMHKKF_16 | 29049 | 30695 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| IMAMHKKF_14 | 27377 | 29947 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IMAMHKKF_9 | 598818 | 599672 | aadK | Streptomycin | 98.36 | 100.00 | M26879 |
| IMAMHKKF_1 | 69772 | 70692 | mph(K) | Spiramycin, Telithromycin | 97.94 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IMAMHKKF_16 | 29049 | 30692 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| IMAMHKKF_1 | 69772 | 70689 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| IMAMHKKF_9 | 598821 | 599672 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| IMAMHKKF_9 | 54209 | 56806 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.71 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IMAMHKKF_9 | 190537 | 191178 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| IMAMHKKF_15 | 530028 | 531209 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IMAMHKKF_15 | 113048 | 113833 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| IMAMHKKF_15 | 111826 | 113022 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| IMAMHKKF_9 | 8364 | 10127 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| IMAMHKKF_15 | 528403 | 529545 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| IMAMHKKF_15 | 102056 | 109192 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.54 | 100.00 | NP_391076 |
| IMAMHKKF_15 | 109212 | 110150 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.51 | 100.00 | NP_391077 |
| IMAMHKKF_15 | 529564 | 530013 | capC | 0.0 | Capsule | Immune modulation | 98.44 | 100.00 | NP_391470 |
| IMAMHKKF_15 | 110178 | 111797 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.47 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IMAMHKKF_10 | 27963 | 26827 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IMAMHKKF_7 | 4312 | 5181 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IMAMHKKF_6 | 794131 | 794907 | CodY | 8.00e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IMAMHKKF_15 | 388040 | 388609 | ClpP | 4.36e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| IMAMHKKF_6 | 294221 | 294613 | spxA1 | 6.29e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| IMAMHKKF_6 | 294221 | 294613 | spx | 3.75e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| IMAMHKKF_4 | 20527 | 20769 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| IMAMHKKF_16 | 77078 | 76881 | CspA | 3.80e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IMAMHKKF_1 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_2 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_3 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_4 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_6 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_7 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_8 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_9 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_10 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_11 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_13 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_14 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_15 | 0.0 | 1.0 | 0.0 |
| IMAMHKKF_16 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IMAMHKKF_3 | 28990 | 49567 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688 |
| IMAMHKKF_6 | 329499 | 338629 | False | Siphoviridae | VOG0275, VOG4602, VOG3304, VOG9667, VOG4603, VOG8241, VOG4552, VOG4603, VOG4600, VOG1309, VOG4566 |
| IMAMHKKF_6 | 346465 | 368986 | False | Siphoviridae | VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG10223, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5008, VOG0641 |
| IMAMHKKF_6 | 428872 | 455359 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)