safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
EELKHGFB_1	2541830	2542684	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
EELKHGFB_1	2523125	2524327	blt	ARO:3003006	blt	100.00	100.00	L32599.1
EELKHGFB_1	2300814	2301983	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
EELKHGFB_1	1317363	1317680	ykkD	ARO:3003064	ykkD	99.05	100.00	AL009126.1
EELKHGFB_1	1317025	1317363	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
EELKHGFB_1	858160	860730	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
EELKHGFB_1	568190	569833	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
EELKHGFB_1	322705	323298	tmrB	ARO:3003059	tmrB	98.48	100.00	AL009126.3
EELKHGFB_1	272178	273611	lmrB	ARO:3002813	lmrB	99.37	100.00	JYFL01000006.1
EELKHGFB_1	259363	260283	mphK	ARO:3004541	mphK	97.71	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
EELKHGFB_1	2541830	2542684	aadK	Streptomycin	100.00	100.00	M26879
EELKHGFB_1	4031353	4032729	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034
EELKHGFB_1	259363	260283	mph(K)	Spiramycin, Telithromycin	97.72	100.00	NC_000964

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
EELKHGFB_1	2541833	2542684	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
EELKHGFB_1	4031356	4032729	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
EELKHGFB_1	568193	569833	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
EELKHGFB_1	259363	260280	mphK	macrolide 2'-phosphotransferase MphK	BLASTX	97.71	100.00	WP_003246254.1
EELKHGFB_1	4028894	4029412	satA	streptothricin N-acetyltransferase SatA	BLASTX	90.75	100.00	WP_003242546.1
EELKHGFB_1	1991888	1994482	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
EELKHGFB_1	3542352	3543494	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
EELKHGFB_1	3543977	3545158	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
EELKHGFB_1	3543513	3543962	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
EELKHGFB_1	1945231	1946994	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
EELKHGFB_1	3136013	3136798	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
EELKHGFB_1	2101437	2102078	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062
EELKHGFB_1	3132177	3133115	dhbB	Bacillibactin	Nutritional/Metabolic factor	99.89	100.00	NP_391077
EELKHGFB_1	3134791	3135987	dhbC	Bacillibactin	Nutritional/Metabolic factor	99.67	100.00	NP_391079
EELKHGFB_1	3125021	3132157	dhbF	Bacillibactin	Nutritional/Metabolic factor	99.58	100.00	NP_391076
EELKHGFB_1	3133143	3134762	dhbE	Bacillibactin	Nutritional/Metabolic factor	99.57	100.00	NP_391078

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
EELKHGFB_1	126806	127942	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
EELKHGFB_1	19074	19943	pdxS	3.01e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
EELKHGFB_1	1630658	1631434	CodY	3.29e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
EELKHGFB_1	3390739	3391308	ClpP	2.44e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
EELKHGFB_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
EELKHGFB_1	1261116	1287224	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
EELKHGFB_1	2473196	2503440	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600
EELKHGFB_1	2599995	2624451	False	Siphoviridae	VOG1561, VOG4574, VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG1266, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG0531

Phigaro v2.4.0 (Database: Jan. 2024 release)