safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	7	17.83

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
BLFJPDAG_1	678147	679316	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
BLFJPDAG_2	513688	514026	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
BLFJPDAG_2	513371	513688	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
BLFJPDAG_1	455797	456999	blt	ARO:3003006	blt	100.00	100.00	L32599.1
BLFJPDAG_1	437436	438290	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
BLFJPDAG_6	407796	409439	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
BLFJPDAG_6	162847	163440	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
BLFJPDAG_6	112344	113783	lmrB	ARO:3002813	lmrB	99.79	100.42	JYFL01000006.1
BLFJPDAG_6	99529	100449	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
BLFJPDAG_5	27447	30017	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
BLFJPDAG_1	437436	438290	aadK	Streptomycin	100.00	100.00	M26879
BLFJPDAG_6	99529	100449	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
BLFJPDAG_4	769718	771094	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
BLFJPDAG_1	437436	438287	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
BLFJPDAG_6	99529	100446	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
BLFJPDAG_4	767200	767718	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
BLFJPDAG_4	769721	771094	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
BLFJPDAG_6	407799	409439	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
BLFJPDAG_1	985634	988228	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
BLFJPDAG_4	280228	281370	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
BLFJPDAG_4	281853	283034	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
BLFJPDAG_4	281389	281838	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
BLFJPDAG_1	1033123	1034886	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
BLFJPDAG_3	112993	113778	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
BLFJPDAG_3	109157	110095	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
BLFJPDAG_3	111771	112967	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
BLFJPDAG_3	110123	111742	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
BLFJPDAG_3	102001	109137	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
BLFJPDAG_1	878021	878662	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BLFJPDAG_7	27995	26859	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
BLFJPDAG_10	11539	10670	pdxS	1.13e-151	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
BLFJPDAG_2	200424	199648	CodY	7.62e-125	nutrient-responsive regulator	81.08	100.00	ACJ80679
BLFJPDAG_4	128616	129185	ClpP	4.85e-99	part of proteolytic complex	81.05	97.44	BAB94595
BLFJPDAG_2	662849	662457	spxA1	2.26e-69	redox-responsive transcription factor	83.21	100.00	AAF21893
BLFJPDAG_9	34596	34354	SpoVG	7.52e-39	RNA-binding protein	81.48	79.41	AAT02994
BLFJPDAG_6	362324	362521	CspA	1.89e-31	cold shock protein	90.91	100.00	CAA62903

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BLFJPDAG_1	0.0	1.0	0.0
BLFJPDAG_2	0.0	1.0	0.0
BLFJPDAG_3	0.0	1.0	0.0
BLFJPDAG_4	0.0	1.0	0.0
BLFJPDAG_5	0.0	1.0	0.0
BLFJPDAG_6	0.0	1.0	0.0
BLFJPDAG_7	0.0	1.0	0.0
BLFJPDAG_9	0.0	1.0	0.0
BLFJPDAG_10	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
BLFJPDAG_1	476550	506926	False	Myoviridae	VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718
BLFJPDAG_1	1163922	1186815	False	Siphoviridae	VOG1659, VOG4918, VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581
BLFJPDAG_2	6884	9467	False	Siphoviridae	VOG4552, VOG0181, VOG5353, VOG9667
BLFJPDAG_2	543865	569973	False	Myoviridae	VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667

Phigaro v2.4.0 (Database: Jan. 2024 release)