Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FJMBKPPO_1 | 678060 | 679229 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| FJMBKPPO_3 | 513688 | 514026 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| FJMBKPPO_3 | 513371 | 513688 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| FJMBKPPO_1 | 455708 | 456910 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| FJMBKPPO_1 | 437347 | 438201 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
| FJMBKPPO_2 | 407915 | 409558 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| FJMBKPPO_2 | 162847 | 163440 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| FJMBKPPO_2 | 112344 | 113783 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| FJMBKPPO_2 | 99529 | 100449 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| FJMBKPPO_6 | 27213 | 29783 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FJMBKPPO_1 | 437347 | 438201 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| FJMBKPPO_2 | 99529 | 100449 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| FJMBKPPO_4 | 518803 | 520179 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FJMBKPPO_1 | 437347 | 438198 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| FJMBKPPO_2 | 99529 | 100446 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| FJMBKPPO_4 | 516285 | 516803 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| FJMBKPPO_4 | 518806 | 520179 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| FJMBKPPO_2 | 407918 | 409558 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| FJMBKPPO_1 | 985547 | 988141 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FJMBKPPO_4 | 29313 | 30455 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| FJMBKPPO_4 | 30938 | 32119 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| FJMBKPPO_4 | 30474 | 30923 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| FJMBKPPO_1 | 1033036 | 1034799 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| FJMBKPPO_5 | 112817 | 113602 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| FJMBKPPO_5 | 108981 | 109919 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391077 |
| FJMBKPPO_5 | 111595 | 112791 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391079 |
| FJMBKPPO_5 | 109947 | 111566 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391078 |
| FJMBKPPO_5 | 101825 | 108961 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391076 |
| FJMBKPPO_1 | 877934 | 878575 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FJMBKPPO_8 | 31692 | 32828 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| FJMBKPPO_11 | 4270 | 5139 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| FJMBKPPO_3 | 200424 | 199648 | CodY | 7.62e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FJMBKPPO_5 | 367541 | 368110 | ClpP | 2.94e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| FJMBKPPO_3 | 662848 | 662456 | spxA1 | 2.26e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| FJMBKPPO_9 | 20926 | 21168 | SpoVG | 7.55e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FJMBKPPO_2 | 362443 | 362640 | CspA | 1.89e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FJMBKPPO_1 | 0.0 | 1.0 | 0.0 |
| FJMBKPPO_2 | 0.0 | 1.0 | 0.0 |
| FJMBKPPO_3 | 0.0 | 1.0 | 0.0 |
| FJMBKPPO_4 | 0.0 | 1.0 | 0.0 |
| FJMBKPPO_5 | 0.0 | 1.0 | 0.0 |
| FJMBKPPO_6 | 0.0 | 1.0 | 0.0 |
| FJMBKPPO_7 | 0.0 | 1.0 | 0.0 |
| FJMBKPPO_8 | 0.0 | 1.0 | 0.0 |
| FJMBKPPO_9 | 0.0 | 1.0 | 0.0 |
| FJMBKPPO_11 | 0.0 | 1.0 | 0.0 |
| FJMBKPPO_12 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FJMBKPPO_1 | 476462 | 506839 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| FJMBKPPO_3 | 6884 | 9467 | False | Siphoviridae | VOG4552, VOG0181, VOG5353, VOG9667 |
| FJMBKPPO_3 | 543865 | 569973 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| FJMBKPPO_7 | 103840 | 126733 | False | Siphoviridae | VOG1659, VOG4918, VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581 |
Phigaro v2.4.0 (Database: Jan. 2024 release)