safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
DOFNABAN_1	2715175	2716029	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
DOFNABAN_1	2696466	2697668	blt	ARO:3003006	blt	100.00	100.00	L32599.1
DOFNABAN_1	2474149	2475318	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
DOFNABAN_1	1356347	1356664	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
DOFNABAN_1	1356009	1356347	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
DOFNABAN_1	896267	898837	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
DOFNABAN_1	584224	585867	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
DOFNABAN_1	339156	339749	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
DOFNABAN_1	288653	290086	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
DOFNABAN_1	275838	276758	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
DOFNABAN_1	2715175	2716029	aadK	Streptomycin	100.00	100.00	M26879
DOFNABAN_1	275838	276758	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
DOFNABAN_1	4167177	4168553	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
DOFNABAN_1	2715178	2716029	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
DOFNABAN_1	275838	276755	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
DOFNABAN_1	4164659	4165177	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
DOFNABAN_1	4167180	4168553	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
DOFNABAN_1	584227	585867	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
DOFNABAN_1	2030826	2033420	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
DOFNABAN_1	3677316	3678458	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
DOFNABAN_1	3678941	3680122	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
DOFNABAN_1	3678477	3678926	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
DOFNABAN_1	1984168	1985931	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
DOFNABAN_1	3271005	3271790	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
DOFNABAN_1	3267169	3268107	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
DOFNABAN_1	3269783	3270979	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
DOFNABAN_1	3268135	3269754	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
DOFNABAN_1	3260013	3267149	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
DOFNABAN_1	2274794	2275435	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
DOFNABAN_1	132882	134018	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
DOFNABAN_1	19074	19943	pdxS	3.12e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
DOFNABAN_1	1669611	1670387	CodY	3.40e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
DOFNABAN_1	3525728	3526297	ClpP	2.52e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
DOFNABAN_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
DOFNABAN_1	1300062	1326170	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
DOFNABAN_1	2646538	2676782	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)