Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DICDKBPA_46 | 100671 | 101840 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| DICDKBPA_132 | 48158 | 50728 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| DICDKBPA_33 | 27532 | 28965 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| DICDKBPA_17 | 4681 | 5019 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| DICDKBPA_17 | 4364 | 4681 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DICDKBPA_64 | 274 | 1128 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DICDKBPA_131 | 79372 | 80748 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.35 | 100.00 | X08034 |
| DICDKBPA_64 | 276 | 1128 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| DICDKBPA_33 | 40854 | 41773 | mph(K) | Spiramycin, Telithromycin | 97.39 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DICDKBPA_131 | 79375 | 80748 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| DICDKBPA_64 | 277 | 1128 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| DICDKBPA_62 | 44124 | 45764 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.62 | 100.00 | WP_003234144.1 |
| DICDKBPA_131 | 76873 | 77391 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DICDKBPA_92 | 32426 | 33364 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| DICDKBPA_158 | 42205 | 43386 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| DICDKBPA_92 | 36262 | 37047 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| DICDKBPA_161 | 1481 | 3244 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| DICDKBPA_158 | 43869 | 45011 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| DICDKBPA_158 | 43401 | 43850 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| DICDKBPA_92 | 35040 | 36236 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391079 |
| DICDKBPA_92 | 25270 | 32406 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.43 | 100.00 | NP_391076 |
| DICDKBPA_38 | 10943 | 11584 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| DICDKBPA_92 | 33392 | 35011 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DICDKBPA_130 | 28028 | 26892 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| DICDKBPA_109 | 4287 | 5156 | pdxS | 1.94e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| DICDKBPA_86 | 156265 | 157041 | CodY | 2.24e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DICDKBPA_138 | 54027 | 54596 | ClpP | 6.86e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DICDKBPA_47 | 51814 | 52206 | spxA1 | 9.75e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DICDKBPA_47 | 51814 | 52206 | spx | 5.82e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DICDKBPA_105 | 34633 | 34391 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DICDKBPA_157 | 4017 | 4214 | CspA | 2.56e-33 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DICDKBPA_38 | 23546 | 23740 | CspD | 1.19e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| DICDKBPA_153 | 190853 | 191047 | CspB | 1.86e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DICDKBPA_1 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_3 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_4 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_5 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_6 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_8 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_9 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_10 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_11 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_12 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_13 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_14 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_15 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_16 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_17 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_18 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_19 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_20 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_21 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_22 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_23 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_24 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_25 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_26 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_27 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_28 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_29 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_30 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_32 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_33 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_34 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_35 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_36 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_37 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_38 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_39 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_40 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_41 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_42 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_43 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_44 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_45 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_46 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_47 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_48 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_50 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_52 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_53 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_54 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_55 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_56 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_58 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_59 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_60 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_61 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_62 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_63 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_64 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_68 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_70 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_71 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_72 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_73 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_74 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_75 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_76 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_78 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_81 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_84 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_85 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_86 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_87 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_89 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_91 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_92 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_93 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_94 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_98 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_99 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_100 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_102 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_103 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_104 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_105 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_106 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_107 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_109 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_110 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_112 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_113 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_114 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_115 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_117 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_118 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_119 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_121 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_122 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_124 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_125 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_126 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_127 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_128 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_130 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_131 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_132 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_133 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_135 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_136 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_138 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_139 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_140 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_141 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_142 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_143 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_144 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_145 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_146 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_147 | 1.0 | 0.0 | 1.0 |
| DICDKBPA_148 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_149 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_150 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_151 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_152 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_153 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_154 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_155 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_157 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_158 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_159 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_160 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_161 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_162 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_163 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_164 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_166 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_167 | 0.0 | 1.0 | 0.0 |
| DICDKBPA_168 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DICDKBPA_17 | 34239 | 60925 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| DICDKBPA_28 | 7686 | 35196 | False | Siphoviridae | VOG9667, VOG1879, VOG0535, VOG0283, VOG11060, VOG0189, VOG0102, VOG6587, VOG5090, VOG7345, VOG5617, VOG0468, VOG8909, VOG6581, VOG6140, VOG0198, VOG0612, VOG3547, VOG0691, VOG0692, VOG9317, VOG4572, VOG2621, VOG5007, VOG0695, VOG0696, VOG0697, VOG6548, VOG2621, VOG2621, VOG10044, VOG0701, VOG4545, VOG4605, VOG4599 |
| DICDKBPA_47 | 94225 | 107978 | False | Siphoviridae | VOG0189, VOG0943, VOG0025, VOG4551, VOG2215, VOG1563, VOG0052, VOG5753, VOG10937, VOG0026 |
| DICDKBPA_47 | 122005 | 149667 | False | Siphoviridae | VOG6495, VOG3699, VOG4600, VOG0275, VOG5023, VOG5012, VOG4555, VOG3676, VOG5504, VOG4564, VOG5589, VOG0704, VOG0799, VOG0725, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0753, VOG4824, VOG0602, VOG6116 |
| DICDKBPA_63 | 35431 | 56236 | False | Myoviridae | VOG4615, VOG0641, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555 |
| DICDKBPA_75 | 6139 | 9639 | False | Unknown | VOG1720, VOG4552, VOG0650, VOG9667, VOG9667 |
| DICDKBPA_115 | 12334 | 27018 | False | Myoviridae / Siphoviridae | VOG7718, VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036, VOG9867, VOG7134, VOG10940, VOG5721, VOG5779, VOG5722 |
| DICDKBPA_115 | 43575 | 55825 | False | Myoviridae | VOG6495, VOG0010, VOG4799, VOG1013, VOG5208, VOG4910, VOG5356, VOG5243, VOG5220, VOG5219, VOG9692, VOG0705, VOG6362, VOG1563 |
| DICDKBPA_126 | 6649 | 23002 | False | Myoviridae | VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862 |
| DICDKBPA_141 | 2205 | 26041 | False | Siphoviridae | VOG9667, VOG9667, VOG2703, VOG4574, VOG0136, VOG0753, VOG5615, VOG5535, VOG3390, VOG4599, VOG4605, VOG4545, VOG1323, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG0815, VOG1125, VOG4553, VOG4555, VOG6778, VOG4564, VOG0720, VOG10227 |
Phigaro v2.4.0 (Database: Jan. 2024 release)