Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FHICNLIJ_55 | 175727 | 176647 | mphK | ARO:3004541 | mphK | 98.04 | 100.00 | CP010314.1 |
| FHICNLIJ_55 | 162396 | 163835 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| FHICNLIJ_72 | 100731 | 101900 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| FHICNLIJ_7 | 85315 | 85653 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| FHICNLIJ_7 | 84998 | 85315 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| FHICNLIJ_50 | 46682 | 49252 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| FHICNLIJ_68 | 865 | 2067 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| FHICNLIJ_92 | 309 | 1163 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FHICNLIJ_92 | 311 | 1163 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| FHICNLIJ_55 | 175727 | 176647 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FHICNLIJ_55 | 175730 | 176647 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.04 | 100.00 | WP_003246254.1 |
| FHICNLIJ_18 | 29012 | 30652 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.99 | 100.00 | WP_003234144.1 |
| FHICNLIJ_92 | 312 | 1163 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| FHICNLIJ_28 | 71945 | 72337 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 93.13 | 75.72 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FHICNLIJ_80 | 275 | 1417 | capA | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | NP_391469 |
| FHICNLIJ_80 | 1900 | 3081 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| FHICNLIJ_35 | 73146 | 74084 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391077 |
| FHICNLIJ_80 | 1436 | 1885 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| FHICNLIJ_35 | 69474 | 70259 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| FHICNLIJ_33 | 29184 | 30947 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| FHICNLIJ_35 | 74104 | 81240 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.82 | 100.00 | NP_391076 |
| FHICNLIJ_35 | 70285 | 71481 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| FHICNLIJ_2 | 111885 | 112526 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.97 | 100.00 | NP_390062 |
| FHICNLIJ_35 | 71499 | 73118 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.84 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FHICNLIJ_26 | 28075 | 26939 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| FHICNLIJ_24 | 4289 | 5158 | pdxS | 1.95e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| FHICNLIJ_54 | 106366 | 107142 | CodY | 1.30e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FHICNLIJ_6 | 45156 | 44587 | ClpP | 1.24e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| FHICNLIJ_12 | 38706 | 38314 | spxA1 | 3.91e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| FHICNLIJ_12 | 38706 | 38314 | spx | 2.33e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| FHICNLIJ_79 | 34633 | 34391 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FHICNLIJ_18 | 71802 | 71605 | CspA | 3.27e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| FHICNLIJ_45 | 4125 | 3931 | CspD | 9.91e-30 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| FHICNLIJ_47 | 47572 | 47766 | CspB | 5.57e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| FHICNLIJ_46 | 3688 | 3881 | Col(SD853) | 194 / 194 | 98.97 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FHICNLIJ_1 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_2 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_4 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_5 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_6 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_7 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_9 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_10 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_11 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_12 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_13 | 1.0 | 0.0 | 1.0 |
| FHICNLIJ_14 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_15 | 1.0 | 0.0 | 1.0 |
| FHICNLIJ_16 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_18 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_19 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_20 | 1.0 | 0.0 | 1.0 |
| FHICNLIJ_24 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_25 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_26 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_27 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_28 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_30 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_31 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_32 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_33 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_34 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_35 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_36 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_37 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_38 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_39 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_40 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_41 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_42 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_43 | 1.0 | 0.0 | 1.0 |
| FHICNLIJ_44 | 1.0 | 0.0 | 1.0 |
| FHICNLIJ_45 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_46 | 1.0 | 0.0 | 1.0 |
| FHICNLIJ_47 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_48 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_49 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_50 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_51 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_52 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_53 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_54 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_55 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_56 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_58 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_59 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_60 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_61 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_62 | 1.0 | 0.0 | 1.0 |
| FHICNLIJ_63 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_64 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_65 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_66 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_67 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_68 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_69 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_70 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_71 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_72 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_73 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_74 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_77 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_78 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_79 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_80 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_81 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_82 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_83 | 1.0 | 0.0 | 1.0 |
| FHICNLIJ_84 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_85 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_86 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_87 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_89 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_90 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_91 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_92 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_93 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_94 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_95 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_96 | 0.0 | 1.0 | 0.0 |
| FHICNLIJ_99 | 1.0 | 0.0 | 1.0 |
| FHICNLIJ_100 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FHICNLIJ_2 | 52161 | 53944 | False | Siphoviridae | VOG6036, VOG5495, VOG9219 |
| FHICNLIJ_2 | 56777 | 77528 | False | Siphoviridae | VOG7718, VOG3390, VOG7895, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG5070, VOG8440 |
| FHICNLIJ_7 | 116304 | 139487 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861 |
Phigaro v2.4.0 (Database: Jan. 2024 release)