Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 9 | 17.38 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LBGLJAEN_22 | 537002 | 538204 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| LBGLJAEN_25 | 345849 | 346769 | mphK | ARO:3004541 | mphK | 97.39 | 100.00 | CP010314.1 |
| LBGLJAEN_25 | 332523 | 333956 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| LBGLJAEN_22 | 312156 | 313325 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| LBGLJAEN_25 | 283000 | 283593 | tmrB | ARO:3003059 | tmrB | 98.98 | 100.00 | AL009126.3 |
| LBGLJAEN_32 | 267811 | 270381 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| LBGLJAEN_9 | 110611 | 110928 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| LBGLJAEN_9 | 110273 | 110611 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| LBGLJAEN_25 | 37035 | 38681 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| LBGLJAEN_23 | 236681 | 238055 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.98 | 99.85 | D12567 |
| LBGLJAEN_25 | 345849 | 346769 | mph(K) | Spiramycin, Telithromycin | 97.94 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LBGLJAEN_25 | 37035 | 38678 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| LBGLJAEN_25 | 345852 | 346769 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.39 | 100.00 | WP_003246254.1 |
| LBGLJAEN_23 | 234180 | 234698 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
| LBGLJAEN_23 | 236684 | 237577 | tet(L) | tetracycline efflux MFS transporter Tet(L) | PARTIALX | 98.66 | 65.07 | WP_063856081.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LBGLJAEN_14 | 99700 | 100638 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| LBGLJAEN_3 | 113810 | 114991 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| LBGLJAEN_22 | 112535 | 113175 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 99.84 | NP_390062 |
| LBGLJAEN_14 | 103525 | 104310 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| LBGLJAEN_14 | 92544 | 99680 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.43 | 100.00 | NP_391076 |
| LBGLJAEN_21 | 307193 | 308956 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| LBGLJAEN_3 | 115474 | 116616 | capA | 0.0 | Capsule | Immune modulation | 98.86 | 100.00 | NP_391469 |
| LBGLJAEN_14 | 102303 | 103499 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391079 |
| LBGLJAEN_3 | 115006 | 115455 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| LBGLJAEN_14 | 100666 | 102285 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.21 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LBGLJAEN_1 | 27907 | 26771 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| LBGLJAEN_5 | 11179 | 10310 | pdxS | 1.10e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| LBGLJAEN_21 | 74035 | 74811 | CodY | 3.83e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| LBGLJAEN_33 | 153521 | 152952 | ClpP | 1.34e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| LBGLJAEN_30 | 292125 | 292517 | spxA1 | 2.15e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| LBGLJAEN_30 | 292125 | 292517 | spx | 1.28e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| LBGLJAEN_7 | 20536 | 20778 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| LBGLJAEN_25 | 84657 | 84460 | CspA | 1.90e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| LBGLJAEN_22 | 125141 | 125335 | CspD | 3.89e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| LBGLJAEN_30 | 33129 | 32935 | CspB | 3.10e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LBGLJAEN_1 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_2 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_3 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_4 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_5 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_7 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_8 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_9 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_11 | 1.0 | 0.0 | 1.0 |
| LBGLJAEN_12 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_14 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_15 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_16 | 1.0 | 0.0 | 1.0 |
| LBGLJAEN_18 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_19 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_20 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_21 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_22 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_23 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_24 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_25 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_26 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_28 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_29 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_30 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_31 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_32 | 0.0 | 1.0 | 0.0 |
| LBGLJAEN_33 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| LBGLJAEN_9 | 54082 | 80457 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| LBGLJAEN_14 | 116758 | 146295 | False | Siphoviridae | VOG8643, VOG2788, VOG0705, VOG7531, VOG7508, VOG0602, VOG4574, VOG0753, VOG4705, VOG4611, VOG4626, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5601, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841, VOG1655 |
| LBGLJAEN_22 | 57462 | 76581 | False | Siphoviridae | VOG8610, VOG7930, VOG0275, VOG4662, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG7900 |
| LBGLJAEN_22 | 488831 | 518974 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796, VOG4673, VOG9667, VOG4693, VOG0189 |
| LBGLJAEN_24 | 174817 | 207411 | False | Siphoviridae | VOG7438, VOG1047, VOG0021, VOG1565, VOG0026, VOG4609, VOG0327, VOG4548, VOG0198, VOG4841, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG3390, VOG4705, VOG0641, VOG4574 |
Phigaro v2.4.0 (Database: Jan. 2024 release)