Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DLFLLOJA_53 | 100073 | 101242 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DLFLLOJA_123 | 99601 | 99918 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| DLFLLOJA_123 | 99263 | 99601 | ykkC | ARO:3003063 | ykkC | 97.32 | 100.00 | AL009126.1 |
| DLFLLOJA_61 | 44904 | 47474 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| DLFLLOJA_6 | 39569 | 41002 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| DLFLLOJA_82 | 273 | 1127 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DLFLLOJA_33 | 34311 | 35687 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.35 | 100.00 | X08034 |
| DLFLLOJA_82 | 275 | 1127 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| DLFLLOJA_43 | 17251 | 18170 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DLFLLOJA_33 | 34311 | 35684 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| DLFLLOJA_74 | 49788 | 51428 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.62 | 100.00 | WP_003234144.1 |
| DLFLLOJA_82 | 276 | 1127 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| DLFLLOJA_33 | 37668 | 38186 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DLFLLOJA_81 | 1639 | 2781 | capA | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | NP_391469 |
| DLFLLOJA_81 | 3264 | 4445 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| DLFLLOJA_81 | 2800 | 3249 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| DLFLLOJA_11 | 103518 | 104303 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| DLFLLOJA_69 | 1480 | 3243 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| DLFLLOJA_11 | 92526 | 99662 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.95 | 100.00 | NP_391076 |
| DLFLLOJA_11 | 102296 | 103492 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391079 |
| DLFLLOJA_11 | 99682 | 100620 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.62 | 100.00 | NP_391077 |
| DLFLLOJA_11 | 100648 | 102267 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.90 | 100.00 | NP_391078 |
| DLFLLOJA_63 | 48217 | 48858 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DLFLLOJA_89 | 31711 | 32847 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| DLFLLOJA_77 | 4285 | 5154 | pdxS | 1.95e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| DLFLLOJA_117 | 156213 | 156989 | CodY | 1.80e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DLFLLOJA_83 | 161158 | 161727 | ClpP | 1.87e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DLFLLOJA_51 | 37152 | 36760 | spxA1 | 5.67e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DLFLLOJA_51 | 37152 | 36760 | spx | 3.38e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DLFLLOJA_55 | 34631 | 34389 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DLFLLOJA_74 | 4014 | 4211 | CspA | 3.48e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DLFLLOJA_63 | 36366 | 36172 | CspD | 7.61e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| DLFLLOJA_133 | 141821 | 142015 | CspB | 1.42e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DLFLLOJA_1 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_2 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_3 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_4 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_6 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_7 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_8 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_9 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_10 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_11 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_12 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_13 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_14 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_15 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_16 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_17 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_18 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_19 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_21 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_22 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_23 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_24 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_25 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_26 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_29 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_31 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_32 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_33 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_34 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_35 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_36 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_38 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_39 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_42 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_43 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_44 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_45 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_46 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_48 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_50 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_51 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_52 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_53 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_54 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_55 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_56 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_57 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_58 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_59 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_60 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_61 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_63 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_65 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_67 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_69 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_70 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_72 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_73 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_74 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_75 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_76 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_77 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_80 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_81 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_82 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_83 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_84 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_85 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_86 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_87 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_88 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_89 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_90 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_91 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_92 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_93 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_94 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_95 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_96 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_97 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_98 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_99 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_100 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_101 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_102 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_103 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_104 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_105 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_106 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_107 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_108 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_109 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_110 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_111 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_112 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_113 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_114 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_115 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_116 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_117 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_118 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_119 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_120 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_121 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_123 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_124 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_125 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_127 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_128 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_129 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_130 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_132 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_133 | 0.0 | 1.0 | 0.0 |
| DLFLLOJA_134 | 1.0 | 0.0 | 1.0 |
| DLFLLOJA_135 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DLFLLOJA_39 | 4218 | 23727 | False | Siphoviridae | VOG0136, VOG0753, VOG10940, VOG5535, VOG4599, VOG4605, VOG9745, VOG0701, VOG10044, VOG6548, VOG0697, VOG0696, VOG0695, VOG2621, VOG4572, VOG9317, VOG0692, VOG0691, VOG10227, VOG0796, VOG7983 |
| DLFLLOJA_42 | 5903 | 23513 | False | Myoviridae | VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG4550, VOG4691, VOG4862, VOG7718 |
| DLFLLOJA_50 | 5856 | 29596 | False | Myoviridae | VOG0796, VOG0720, VOG4555, VOG4553, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG0753, VOG0136, VOG4574 |
| DLFLLOJA_75 | 4255 | 5975 | False | Unknown | VOG0189, VOG6809, VOG6587, VOG5090 |
| DLFLLOJA_75 | 6937 | 29664 | False | Siphoviridae | VOG7345, VOG5617, VOG0468, VOG8909, VOG6581, VOG6140, VOG0198, VOG0612, VOG0691, VOG0692, VOG5615, VOG9317, VOG4572, VOG5007, VOG0695, VOG0696, VOG0697, VOG0698, VOG2621, VOG10044, VOG0701, VOG4545, VOG4605, VOG4599, VOG0641 |
| DLFLLOJA_80 | 18546 | 43167 | False | Myoviridae | VOG1328, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG1581, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4691, VOG4862, VOG0906, VOG7718 |
| DLFLLOJA_123 | 44263 | 70007 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705, VOG0753 |
Phigaro v2.4.0 (Database: Jan. 2024 release)