Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 8 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MGIJIKFF_38 | 123453 | 124892 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| MGIJIKFF_72 | 36172 | 37374 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| MGIJIKFF_72 | 17757 | 18611 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| MGIJIKFF_57 | 9391 | 11961 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| MGIJIKFF_36 | 9060 | 10229 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| MGIJIKFF_23 | 6824 | 7162 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| MGIJIKFF_23 | 6507 | 6824 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MGIJIKFF_3 | 50746 | 52122 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.77 | 100.00 | D12567 |
| MGIJIKFF_72 | 17757 | 18609 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| MGIJIKFF_38 | 97180 | 98099 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MGIJIKFF_3 | 50749 | 52122 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| MGIJIKFF_11 | 29058 | 30698 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.62 | 100.00 | WP_003234144.1 |
| MGIJIKFF_72 | 17757 | 18608 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| MGIJIKFF_3 | 48253 | 48771 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MGIJIKFF_63 | 99746 | 100684 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| MGIJIKFF_35 | 310191 | 311372 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| MGIJIKFF_35 | 311855 | 312997 | capA | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391469 |
| MGIJIKFF_35 | 311387 | 311836 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| MGIJIKFF_63 | 103571 | 104356 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| MGIJIKFF_43 | 84544 | 86307 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| MGIJIKFF_63 | 92590 | 99726 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.78 | 100.00 | NP_391076 |
| MGIJIKFF_63 | 102349 | 103545 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| MGIJIKFF_59 | 178083 | 178724 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| MGIJIKFF_63 | 100712 | 102331 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MGIJIKFF_1 | 28005 | 26869 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| MGIJIKFF_39 | 4297 | 5166 | pdxS | 1.94e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| MGIJIKFF_52 | 150522 | 151298 | CodY | 2.13e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MGIJIKFF_63 | 365123 | 365692 | ClpP | 3.10e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MGIJIKFF_73 | 51963 | 52355 | spxA1 | 5.77e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MGIJIKFF_73 | 51963 | 52355 | spx | 3.44e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MGIJIKFF_30 | 34642 | 34400 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MGIJIKFF_11 | 75866 | 75669 | CspA | 3.45e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MGIJIKFF_59 | 166231 | 166037 | CspD | 5.39e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| MGIJIKFF_59 | 166231 | 166034 | CspB | 1.12e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| MGIJIKFF_21 | 3653 | 3846 | Col(SD853) | 194 / 194 | 100.00 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MGIJIKFF_1 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_2 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_3 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_4 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_5 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_6 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_9 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_10 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_11 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_13 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_14 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_15 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_16 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_17 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_18 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_19 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_21 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_23 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_24 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_25 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_26 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_28 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_29 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_30 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_31 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_32 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_33 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_34 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_35 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_36 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_37 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_38 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_39 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_40 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_41 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_42 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_43 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_44 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_45 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_46 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_47 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_48 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_49 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_50 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_51 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_52 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_53 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_54 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_55 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_56 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_57 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_59 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_60 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_61 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_62 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_63 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_64 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_65 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_66 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_67 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_68 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_69 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_70 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_71 | 1.0 | 0.0 | 1.0 |
| MGIJIKFF_72 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_73 | 0.0 | 1.0 | 0.0 |
| MGIJIKFF_74 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MGIJIKFF_14 | 35186 | 53565 | False | Siphoviridae | VOG5722, VOG5779, VOG5721, VOG10940, VOG7134, VOG9867, VOG6036, VOG5495, VOG9219, VOG9713, VOG6940, VOG5534, VOG7718, VOG3390, VOG4705, VOG5008, VOG8610, VOG0275 |
| MGIJIKFF_23 | 35468 | 62238 | False | Myoviridae | VOG4707, VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| MGIJIKFF_55 | 110883 | 136071 | False | Siphoviridae | VOG2703, VOG1993, VOG4574, VOG0641, VOG4705, VOG6243, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG4841 |
Phigaro v2.4.0 (Database: Jan. 2024 release)