Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| POCDONPD_12 | 112690 | 114129 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| POCDONPD_87 | 100741 | 101910 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| POCDONPD_74 | 99891 | 100745 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| POCDONPD_12 | 99878 | 100798 | mphK | ARO:3004541 | mphK | 98.04 | 100.00 | CP010314.1 |
| POCDONPD_1 | 83334 | 83651 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| POCDONPD_1 | 82996 | 83334 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| POCDONPD_63 | 46569 | 49139 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| POCDONPD_84 | 11918 | 13120 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| POCDONPD_74 | 99891 | 100743 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| POCDONPD_12 | 99878 | 100798 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| POCDONPD_12 | 99878 | 100795 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.04 | 100.00 | WP_003246254.1 |
| POCDONPD_80 | 45674 | 47314 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.99 | 100.00 | WP_003234144.1 |
| POCDONPD_74 | 99891 | 100742 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| POCDONPD_2 | 34787 | 35179 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 93.13 | 75.72 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| POCDONPD_76 | 9877 | 11019 | capA | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | NP_391469 |
| POCDONPD_76 | 8213 | 9394 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| POCDONPD_65 | 59330 | 60268 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391077 |
| POCDONPD_76 | 9409 | 9858 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| POCDONPD_65 | 63155 | 63940 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| POCDONPD_27 | 29188 | 30951 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| POCDONPD_65 | 52174 | 59310 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.82 | 100.00 | NP_391076 |
| POCDONPD_65 | 61933 | 63129 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| POCDONPD_91 | 15189 | 15830 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.97 | 100.00 | NP_390062 |
| POCDONPD_65 | 60296 | 61915 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.84 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| POCDONPD_95 | 28079 | 26943 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| POCDONPD_75 | 11279 | 10410 | pdxS | 1.95e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| POCDONPD_22 | 53816 | 53040 | CodY | 1.30e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| POCDONPD_62 | 45163 | 44594 | ClpP | 1.24e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| POCDONPD_28 | 38707 | 38315 | spxA1 | 3.93e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| POCDONPD_28 | 38707 | 38315 | spx | 2.34e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| POCDONPD_56 | 20541 | 20783 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| POCDONPD_80 | 4524 | 4721 | CspA | 3.30e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| POCDONPD_79 | 4129 | 3935 | CspD | 9.91e-30 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| POCDONPD_44 | 47576 | 47770 | CspB | 5.57e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| POCDONPD_72 | 2196 | 2389 | Col(SD853) | 194 / 194 | 98.97 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| POCDONPD_1 | 0.0 | 1.0 | 0.0 |
| POCDONPD_2 | 0.0 | 1.0 | 0.0 |
| POCDONPD_4 | 0.0 | 1.0 | 0.0 |
| POCDONPD_5 | 0.0 | 1.0 | 0.0 |
| POCDONPD_8 | 1.0 | 0.0 | 1.0 |
| POCDONPD_9 | 0.0 | 1.0 | 0.0 |
| POCDONPD_10 | 0.0 | 1.0 | 0.0 |
| POCDONPD_11 | 0.0 | 1.0 | 0.0 |
| POCDONPD_12 | 0.0 | 1.0 | 0.0 |
| POCDONPD_14 | 1.0 | 0.0 | 1.0 |
| POCDONPD_15 | 0.0 | 1.0 | 0.0 |
| POCDONPD_16 | 0.0 | 1.0 | 0.0 |
| POCDONPD_17 | 0.0 | 1.0 | 0.0 |
| POCDONPD_18 | 0.0 | 1.0 | 0.0 |
| POCDONPD_19 | 1.0 | 0.0 | 1.0 |
| POCDONPD_20 | 1.0 | 0.0 | 1.0 |
| POCDONPD_21 | 1.0 | 0.0 | 1.0 |
| POCDONPD_22 | 0.0 | 1.0 | 0.0 |
| POCDONPD_24 | 0.0 | 1.0 | 0.0 |
| POCDONPD_25 | 0.0 | 1.0 | 0.0 |
| POCDONPD_26 | 0.0 | 1.0 | 0.0 |
| POCDONPD_27 | 0.0 | 1.0 | 0.0 |
| POCDONPD_28 | 0.0 | 1.0 | 0.0 |
| POCDONPD_30 | 0.0 | 1.0 | 0.0 |
| POCDONPD_31 | 0.0 | 1.0 | 0.0 |
| POCDONPD_33 | 0.0 | 1.0 | 0.0 |
| POCDONPD_34 | 0.0 | 1.0 | 0.0 |
| POCDONPD_36 | 0.0 | 1.0 | 0.0 |
| POCDONPD_37 | 0.0 | 1.0 | 0.0 |
| POCDONPD_38 | 1.0 | 0.0 | 1.0 |
| POCDONPD_39 | 0.0 | 1.0 | 0.0 |
| POCDONPD_40 | 0.0 | 1.0 | 0.0 |
| POCDONPD_41 | 0.0 | 1.0 | 0.0 |
| POCDONPD_42 | 0.0 | 1.0 | 0.0 |
| POCDONPD_43 | 0.0 | 1.0 | 0.0 |
| POCDONPD_44 | 0.0 | 1.0 | 0.0 |
| POCDONPD_45 | 0.0 | 1.0 | 0.0 |
| POCDONPD_46 | 0.0 | 1.0 | 0.0 |
| POCDONPD_47 | 0.0 | 1.0 | 0.0 |
| POCDONPD_48 | 0.0 | 1.0 | 0.0 |
| POCDONPD_49 | 0.0 | 1.0 | 0.0 |
| POCDONPD_50 | 0.0 | 1.0 | 0.0 |
| POCDONPD_52 | 0.0 | 1.0 | 0.0 |
| POCDONPD_53 | 0.0 | 1.0 | 0.0 |
| POCDONPD_54 | 0.0 | 1.0 | 0.0 |
| POCDONPD_55 | 0.0 | 1.0 | 0.0 |
| POCDONPD_56 | 0.0 | 1.0 | 0.0 |
| POCDONPD_57 | 0.0 | 1.0 | 0.0 |
| POCDONPD_58 | 0.0 | 1.0 | 0.0 |
| POCDONPD_60 | 0.0 | 1.0 | 0.0 |
| POCDONPD_61 | 0.0 | 1.0 | 0.0 |
| POCDONPD_62 | 0.0 | 1.0 | 0.0 |
| POCDONPD_63 | 0.0 | 1.0 | 0.0 |
| POCDONPD_65 | 0.0 | 1.0 | 0.0 |
| POCDONPD_66 | 0.0 | 1.0 | 0.0 |
| POCDONPD_67 | 0.0 | 1.0 | 0.0 |
| POCDONPD_69 | 0.0 | 1.0 | 0.0 |
| POCDONPD_70 | 0.0 | 1.0 | 0.0 |
| POCDONPD_71 | 0.0 | 1.0 | 0.0 |
| POCDONPD_72 | 1.0 | 0.0 | 1.0 |
| POCDONPD_73 | 0.0 | 1.0 | 0.0 |
| POCDONPD_74 | 0.0 | 1.0 | 0.0 |
| POCDONPD_75 | 0.0 | 1.0 | 0.0 |
| POCDONPD_76 | 0.0 | 1.0 | 0.0 |
| POCDONPD_77 | 0.0 | 1.0 | 0.0 |
| POCDONPD_78 | 0.0 | 1.0 | 0.0 |
| POCDONPD_79 | 0.0 | 1.0 | 0.0 |
| POCDONPD_80 | 0.0 | 1.0 | 0.0 |
| POCDONPD_81 | 0.0 | 1.0 | 0.0 |
| POCDONPD_82 | 0.0 | 1.0 | 0.0 |
| POCDONPD_84 | 0.0 | 1.0 | 0.0 |
| POCDONPD_85 | 0.0 | 1.0 | 0.0 |
| POCDONPD_86 | 0.0 | 1.0 | 0.0 |
| POCDONPD_87 | 0.0 | 1.0 | 0.0 |
| POCDONPD_88 | 0.0 | 1.0 | 0.0 |
| POCDONPD_89 | 0.0 | 1.0 | 0.0 |
| POCDONPD_90 | 0.0 | 1.0 | 0.0 |
| POCDONPD_91 | 0.0 | 1.0 | 0.0 |
| POCDONPD_92 | 0.0 | 1.0 | 0.0 |
| POCDONPD_94 | 0.0 | 1.0 | 0.0 |
| POCDONPD_95 | 0.0 | 1.0 | 0.0 |
| POCDONPD_96 | 0.0 | 1.0 | 0.0 |
| POCDONPD_97 | 0.0 | 1.0 | 0.0 |
| POCDONPD_98 | 0.0 | 1.0 | 0.0 |
| POCDONPD_99 | 0.0 | 1.0 | 0.0 |
| POCDONPD_100 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| POCDONPD_1 | 29162 | 52345 | False | Myoviridae | VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| POCDONPD_24 | 40930 | 65232 | False | Siphoviridae | VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641, VOG4574 |
| POCDONPD_57 | 106900 | 123447 | False | Siphoviridae | VOG3390, VOG4705, VOG5008, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042 |
| POCDONPD_70 | 16906 | 38872 | False | Myoviridae | VOG0720, VOG4555, VOG4553, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG0641, VOG4574, VOG1993 |
Phigaro v2.4.0 (Database: Jan. 2024 release)