Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ILFOANPM_115 | 67887 | 68741 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| ILFOANPM_17 | 27536 | 28969 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| ILFOANPM_117 | 9053 | 10222 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| ILFOANPM_29 | 4905 | 7475 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| ILFOANPM_87 | 4640 | 4978 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| ILFOANPM_87 | 4323 | 4640 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ILFOANPM_97 | 79380 | 80756 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.35 | 100.00 | X08034 |
| ILFOANPM_115 | 67887 | 68739 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| ILFOANPM_17 | 40858 | 41777 | mph(K) | Spiramycin, Telithromycin | 97.39 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ILFOANPM_97 | 79383 | 80756 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| ILFOANPM_115 | 67887 | 68738 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| ILFOANPM_47 | 44252 | 45892 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.62 | 100.00 | WP_003234144.1 |
| ILFOANPM_97 | 76881 | 77399 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ILFOANPM_24 | 32363 | 33301 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| ILFOANPM_91 | 26007 | 27188 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| ILFOANPM_24 | 36199 | 36984 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| ILFOANPM_114 | 1485 | 3248 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| ILFOANPM_91 | 24382 | 25524 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| ILFOANPM_91 | 25543 | 25992 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| ILFOANPM_24 | 34977 | 36173 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391079 |
| ILFOANPM_24 | 25207 | 32343 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.43 | 100.00 | NP_391076 |
| ILFOANPM_78 | 181978 | 182619 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| ILFOANPM_24 | 33329 | 34948 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ILFOANPM_94 | 28032 | 26896 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| ILFOANPM_124 | 11183 | 10314 | pdxS | 1.94e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| ILFOANPM_61 | 156207 | 156983 | CodY | 2.24e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ILFOANPM_148 | 60408 | 59839 | ClpP | 6.86e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| ILFOANPM_57 | 51919 | 52311 | spxA1 | 9.96e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| ILFOANPM_57 | 51919 | 52311 | spx | 5.94e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| ILFOANPM_120 | 20539 | 20781 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| ILFOANPM_44 | 1944 | 1747 | CspA | 2.56e-33 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| ILFOANPM_78 | 170016 | 169822 | CspD | 1.19e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| ILFOANPM_25 | 190944 | 191138 | CspB | 1.86e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ILFOANPM_1 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_2 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_3 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_6 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_7 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_8 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_9 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_10 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_11 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_12 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_13 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_14 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_15 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_16 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_17 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_18 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_20 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_21 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_22 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_23 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_24 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_25 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_26 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_27 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_28 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_29 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_30 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_31 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_32 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_33 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_35 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_37 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_38 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_39 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_40 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_41 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_42 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_43 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_44 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_46 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_47 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_48 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_49 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_50 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_51 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_53 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_54 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_55 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_57 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_58 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_59 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_60 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_61 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_62 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_63 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_64 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_65 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_66 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_67 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_68 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_70 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_71 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_72 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_74 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_75 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_76 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_77 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_78 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_79 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_81 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_84 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_85 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_87 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_88 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_89 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_90 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_91 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_92 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_94 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_95 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_96 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_97 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_98 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_99 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_100 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_103 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_104 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_105 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_106 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_107 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_108 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_109 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_110 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_111 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_112 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_113 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_114 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_115 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_116 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_117 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_118 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_119 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_120 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_121 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_124 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_125 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_126 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_127 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_128 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_129 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_130 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_131 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_132 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_134 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_135 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_136 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_137 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_138 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_139 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_140 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_141 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_142 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_143 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_146 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_147 | 1.0 | 0.0 | 1.0 |
| ILFOANPM_148 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_149 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_150 | 0.0 | 1.0 | 0.0 |
| ILFOANPM_152 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ILFOANPM_9 | 3715 | 31225 | False | Siphoviridae | VOG4599, VOG4605, VOG4545, VOG0701, VOG10044, VOG2621, VOG2621, VOG6548, VOG0697, VOG0696, VOG0695, VOG5007, VOG2621, VOG4572, VOG9317, VOG0692, VOG0691, VOG3547, VOG0612, VOG0198, VOG6140, VOG6581, VOG8909, VOG0468, VOG5617, VOG7345, VOG5090, VOG6587, VOG0102, VOG0189, VOG11060, VOG0283, VOG0535, VOG1879 |
| ILFOANPM_35 | 35435 | 58204 | False | Myoviridae | VOG4615, VOG0641, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555, VOG0720 |
| ILFOANPM_57 | 94223 | 107934 | False | Siphoviridae | VOG0189, VOG0943, VOG0025, VOG4551, VOG2215, VOG1563, VOG0052, VOG5753, VOG10937, VOG0026 |
| ILFOANPM_57 | 121961 | 149623 | False | Siphoviridae | VOG6495, VOG3699, VOG4600, VOG0275, VOG5023, VOG5012, VOG4555, VOG3676, VOG5504, VOG4564, VOG5589, VOG0704, VOG0799, VOG0725, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0753, VOG4824, VOG0602, VOG6116 |
| ILFOANPM_79 | 1812 | 25648 | False | Siphoviridae | VOG0796, VOG10227, VOG0720, VOG4564, VOG6778, VOG4555, VOG4553, VOG1125, VOG0815, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG1323, VOG4545, VOG4605, VOG4599, VOG3390, VOG5535, VOG5615, VOG0753, VOG0136, VOG4574, VOG2703, VOG9667 |
| ILFOANPM_87 | 34198 | 60884 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| ILFOANPM_89 | 6092 | 9592 | False | Unknown | VOG1720, VOG4552, VOG0650, VOG9667, VOG9667 |
| ILFOANPM_105 | 7127 | 19156 | False | Myoviridae / Siphoviridae | VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036, VOG9867, VOG7134, VOG10940, VOG5721, VOG5779, VOG5722 |
| ILFOANPM_105 | 35722 | 47972 | False | Myoviridae | VOG6495, VOG0010, VOG4799, VOG1013, VOG5208, VOG4910, VOG5356, VOG5243, VOG5220, VOG5219, VOG9692, VOG0705, VOG6362, VOG1563 |
| ILFOANPM_137 | 8217 | 24570 | False | Myoviridae | VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862 |
Phigaro v2.4.0 (Database: Jan. 2024 release)