Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DLLEOLKA_1 | 606583 | 607437 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| DLLEOLKA_10 | 596896 | 597234 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DLLEOLKA_10 | 596579 | 596896 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DLLEOLKA_1 | 589514 | 590716 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| DLLEOLKA_7 | 396056 | 397699 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| DLLEOLKA_1 | 359653 | 360822 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DLLEOLKA_7 | 154931 | 155524 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| DLLEOLKA_7 | 104405 | 105844 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| DLLEOLKA_7 | 91592 | 92512 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| DLLEOLKA_13 | 27394 | 29964 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DLLEOLKA_7 | 91592 | 92512 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| DLLEOLKA_1 | 606583 | 607437 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DLLEOLKA_7 | 91592 | 92509 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| DLLEOLKA_1 | 606586 | 607437 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| DLLEOLKA_7 | 396059 | 397699 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| DLLEOLKA_6 | 38078 | 38596 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.22 | 100.00 | WP_003242546.1 |
| DLLEOLKA_1 | 63951 | 66548 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.65 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DLLEOLKA_6 | 537210 | 538391 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| DLLEOLKA_1 | 162147 | 162788 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| DLLEOLKA_6 | 960330 | 961115 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| DLLEOLKA_6 | 961141 | 962337 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| DLLEOLKA_1 | 9707 | 11470 | dep/capD | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | NP_389723 |
| DLLEOLKA_6 | 538406 | 538855 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| DLLEOLKA_6 | 538874 | 540016 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| DLLEOLKA_6 | 964971 | 972107 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.74 | 100.00 | NP_391076 |
| DLLEOLKA_6 | 964013 | 964951 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| DLLEOLKA_6 | 962366 | 963985 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.35 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DLLEOLKA_9 | 27985 | 26849 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DLLEOLKA_16 | 4544 | 5413 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DLLEOLKA_10 | 285465 | 284689 | CodY | 8.30e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DLLEOLKA_6 | 697606 | 697037 | ClpP | 6.46e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DLLEOLKA_10 | 746456 | 746064 | spxA1 | 6.53e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DLLEOLKA_10 | 746456 | 746064 | spx | 3.89e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DLLEOLKA_17 | 34712 | 34470 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DLLEOLKA_7 | 347642 | 347839 | CspA | 1.84e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DLLEOLKA_1 | 174020 | 174214 | CspD | 2.10e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| DLLEOLKA_1 | 174020 | 174217 | CspB | 4.35e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DLLEOLKA_1 | 0.0 | 1.0 | 0.0 |
| DLLEOLKA_3 | 0.0 | 1.0 | 0.0 |
| DLLEOLKA_5 | 0.0 | 1.0 | 0.0 |
| DLLEOLKA_6 | 0.0 | 1.0 | 0.0 |
| DLLEOLKA_7 | 0.0 | 1.0 | 0.0 |
| DLLEOLKA_9 | 0.0 | 1.0 | 0.0 |
| DLLEOLKA_10 | 0.0 | 1.0 | 0.0 |
| DLLEOLKA_11 | 0.0 | 1.0 | 0.0 |
| DLLEOLKA_12 | 0.0 | 1.0 | 0.0 |
| DLLEOLKA_13 | 0.0 | 1.0 | 0.0 |
| DLLEOLKA_16 | 0.0 | 1.0 | 0.0 |
| DLLEOLKA_17 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DLLEOLKA_1 | 537790 | 560206 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773 |
| DLLEOLKA_1 | 667806 | 691235 | False | Siphoviridae | VOG4574, VOG0641, VOG4705, VOG3390, VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG4841 |
| DLLEOLKA_1 | 699104 | 700266 | False | Unknown | VOG0650, VOG9667 |
| DLLEOLKA_5 | 14700 | 38525 | False | Myoviridae | VOG4681, VOG0796, VOG0720, VOG4555, VOG4553, VOG0815, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG0753, VOG0136, VOG4574 |
| DLLEOLKA_10 | 626784 | 653096 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)