Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 20 | 37 | 42.06 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HMOBGBMI_6 | 24819 | 25550 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 94.81 | 100.00 | WP_011681176 |
| HMOBGBMI_6 | 24819 | 25550 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 94.54 | 100.00 | WP_011226048 |
| HMOBGBMI_15 | 182993 | 183844 | rfbD | 0.0 | Capsule | Immune modulation | 94.13 | 100.00 | WP_014621820 |
| HMOBGBMI_6 | 24819 | 25550 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.13 | 100.00 | WP_011227248 |
| HMOBGBMI_15 | 186106 | 186915 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 92.85 | 99.88 | WP_002948020 |
| HMOBGBMI_15 | 189172 | 190928 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.60 | 98.43 | WP_011226325 |
| HMOBGBMI_15 | 186915 | 188120 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.37 | 100.00 | WP_011226326 |
| HMOBGBMI_15 | 185166 | 186122 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 91.85 | 100.00 | WP_011226328 |
| HMOBGBMI_15 | 184021 | 185169 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 91.57 | 99.83 | WP_041828290 |
| HMOBGBMI_12 | 118091 | 118684 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| HMOBGBMI_14 | 3542 | 5200 | fbp54 | 0.0 | FBPs | Adherence | 91.08 | 99.94 | WP_011227179 |
| HMOBGBMI_15 | 188141 | 189175 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.00 | 99.15 | WP_002948022 |
| HMOBGBMI_6 | 25551 | 27011 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 90.92 | 99.86 | WP_011681177 |
| HMOBGBMI_12 | 118091 | 118684 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| HMOBGBMI_12 | 118091 | 118684 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| HMOBGBMI_6 | 25551 | 27011 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.78 | 100.00 | WP_011226049 |
| HMOBGBMI_6 | 25551 | 27011 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 89.97 | 100.00 | WP_041827043 |
| HMOBGBMI_6 | 24118 | 24810 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| HMOBGBMI_6 | 24118 | 24810 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| HMOBGBMI_6 | 24118 | 24810 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| HMOBGBMI_10 | 127147 | 128450 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| HMOBGBMI_15 | 88480 | 89319 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.87 | 99.88 | WP_011226733 |
| HMOBGBMI_3 | 73660 | 74670 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.28 | 99.80 | WP_002986042 |
| HMOBGBMI_18 | 4330 | 5091 | srtA | 0.0 | Sortase A | Exoenzyme | 83.75 | 99.87 | - |
| HMOBGBMI_12 | 116775 | 117821 | rfbB | 0.0 | Capsule | Immune modulation | 83.49 | 99.90 | WP_002263087 |
| HMOBGBMI_4 | 3520 | 4803 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.53 | 99.61 | WP_000107753 |
| HMOBGBMI_12 | 118684 | 119553 | rfbA | 0.0 | Capsule | Immune modulation | 80.28 | 99.77 | WP_002904719 |
| HMOBGBMI_6 | 23438 | 24108 | STU_RS14635 | 3.39e-148 | Capsule | Immune modulation | 80.98 | 90.28 | WP_011226046 |
| HMOBGBMI_6 | 23438 | 24108 | STER_RS05305 | 1.58e-146 | Capsule | Immune modulation | 80.83 | 90.28 | WP_011681174 |
| HMOBGBMI_6 | 23506 | 24108 | STR_RS05250 | 1.60e-136 | Capsule | Immune modulation | 81.46 | 80.13 | WP_011227246 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HMOBGBMI_18 | 1640 | 657 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| HMOBGBMI_18 | 13227 | 14300 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| HMOBGBMI_10 | 450118 | 452424 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.41 | 99.22 | AAK74619 |
| HMOBGBMI_18 | 32283 | 34529 | metE | 0.0 | methionine biosynthesis enzymes | 93.19 | 100.00 | AAK74738 |
| HMOBGBMI_10 | 225311 | 224343 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| HMOBGBMI_16 | 67638 | 66157 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.92 | 100.00 | ABJ54480 |
| HMOBGBMI_17 | 647 | 1618 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| HMOBGBMI_16 | 146903 | 153376 | PrtA | 0.0 | serine protease | 91.44 | 100.61 | ABJ55268 |
| HMOBGBMI_12 | 146103 | 144373 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| HMOBGBMI_15 | 169362 | 171350 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.74 | 100.00 | AE014259_2 |
| HMOBGBMI_10 | 303393 | 302242 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| HMOBGBMI_11 | 105292 | 103814 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| HMOBGBMI_10 | 128450 | 127149 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| HMOBGBMI_11 | 89942 | 88050 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| HMOBGBMI_18 | 37447 | 35738 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| HMOBGBMI_17 | 35611 | 36702 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| HMOBGBMI_7 | 28456 | 29466 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| HMOBGBMI_14 | 19127 | 17766 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| HMOBGBMI_10 | 142864 | 141974 | bglB | 5.08e-177 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| HMOBGBMI_18 | 34719 | 35576 | metF | 3.88e-170 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| HMOBGBMI_18 | 78161 | 77199 | SP_0676 | 9.20e-170 | transcription facot | 80.69 | 99.69 | AAK74821 |
| HMOBGBMI_10 | 27962 | 28819 | mtsA | 1.34e-151 | substrate-binding lipoprotein of manganese transporter | 80.49 | 92.86 | AIK76191 |
| HMOBGBMI_21 | 145921 | 146601 | NisR | 4.07e-132 | two-component signal-transduction systems | 94.27 | 100.00 | ABP89873 |
| HMOBGBMI_21 | 146589 | 147293 | NisK | 1.01e-130 | two-component signal-transduction systems | 90.64 | 100.00 | ABP89872 |
| HMOBGBMI_16 | 128923 | 129594 | rr01 | 4.19e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| HMOBGBMI_12 | 4559 | 3855 | covR | 6.95e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| HMOBGBMI_18 | 42876 | 43547 | ciaR | 1.13e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| HMOBGBMI_18 | 42876 | 43544 | CiaR | 2.08e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| HMOBGBMI_12 | 4559 | 3855 | walR | 1.58e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| HMOBGBMI_4 | 153945 | 154631 | CovR | 1.78e-120 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| HMOBGBMI_9 | 277 | 672 | SpxA2 | 6.06e-84 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| HMOBGBMI_19 | 7507 | 7908 | SpxA1 | 3.94e-70 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| HMOBGBMI_5 | 47457 | 47870 | GrpE | 4.80e-69 | protein grpE | 82.61 | 80.59 | ABP89267 |
| HMOBGBMI_4 | 177149 | 177436 | spr0479 | 5.62e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| HMOBGBMI_10 | 261086 | 260832 | spr1327 | 1.75e-43 | essential gene | 90.59 | 100.00 | AAL00131 |
| HMOBGBMI_11 | 50556 | 50819 | spr0175 | 6.78e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| HMOBGBMI_11 | 51381 | 51647 | spr0177 | 1.51e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| HMOBGBMI_4 | 4970 | 5461 | RopE (M5005_Spy_1611) | 1.03e-72 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| HMOBGBMI_10 | 450118 | 452418 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| HMOBGBMI_11 | 1432 | 413 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HMOBGBMI_1 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_2 | 1.0 | 0.0 | 1.0 |
| HMOBGBMI_3 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_4 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_5 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_6 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_7 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_8 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_9 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_10 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_11 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_12 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_13 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_14 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_15 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_16 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_17 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_18 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_19 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_20 | 0.0 | 1.0 | 0.0 |
| HMOBGBMI_21 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)