safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
MDJIFCBJ_1	2735683	2736537	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
MDJIFCBJ_1	2716974	2718176	blt	ARO:3003006	blt	100.00	100.00	L32599.1
MDJIFCBJ_1	2494655	2495824	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
MDJIFCBJ_1	1376858	1377175	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
MDJIFCBJ_1	1376520	1376858	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
MDJIFCBJ_1	916776	919346	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
MDJIFCBJ_1	604733	606376	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
MDJIFCBJ_1	339154	339747	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
MDJIFCBJ_1	288651	290084	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
MDJIFCBJ_1	275836	276756	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
MDJIFCBJ_1	2735683	2736537	aadK	Streptomycin	100.00	100.00	M26879
MDJIFCBJ_1	275836	276756	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
MDJIFCBJ_1	4187708	4189084	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
MDJIFCBJ_1	2735686	2736537	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
MDJIFCBJ_1	275836	276753	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
MDJIFCBJ_1	4185190	4185708	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
MDJIFCBJ_1	4187711	4189084	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
MDJIFCBJ_1	604736	606376	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
MDJIFCBJ_1	2051340	2053934	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
MDJIFCBJ_1	3697847	3698989	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
MDJIFCBJ_1	3699472	3700653	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
MDJIFCBJ_1	3699008	3699457	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
MDJIFCBJ_1	2004682	2006445	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
MDJIFCBJ_1	3291512	3292297	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
MDJIFCBJ_1	3287676	3288614	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
MDJIFCBJ_1	3290290	3291486	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
MDJIFCBJ_1	3288642	3290261	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
MDJIFCBJ_1	3280520	3287656	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
MDJIFCBJ_1	2295308	2295949	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
MDJIFCBJ_1	132881	134017	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
MDJIFCBJ_1	19073	19942	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
MDJIFCBJ_1	1690122	1690898	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
MDJIFCBJ_1	3546235	3546804	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
MDJIFCBJ_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
MDJIFCBJ_1	1320573	1346681	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
MDJIFCBJ_1	2667045	2697289	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)