Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 7 | 17.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OEEIJDKC_7 | 618182 | 618520 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OEEIJDKC_7 | 617865 | 618182 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| OEEIJDKC_8 | 561055 | 561909 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| OEEIJDKC_8 | 541874 | 543076 | blt | ARO:3003006 | blt | 98.75 | 100.00 | L32599.1 |
| OEEIJDKC_9 | 411939 | 413582 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| OEEIJDKC_8 | 369940 | 371109 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| OEEIJDKC_10 | 272677 | 275247 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| OEEIJDKC_9 | 161704 | 162297 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| OEEIJDKC_9 | 111146 | 112579 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| OEEIJDKC_9 | 98331 | 99251 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OEEIJDKC_6 | 34578 | 35954 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.42 | 100.00 | D12567 |
| OEEIJDKC_9 | 98331 | 99251 | mph(K) | Spiramycin, Telithromycin | 99.13 | 100.00 | NC_000964 |
| OEEIJDKC_8 | 561057 | 561909 | aadK | Streptomycin | 98.12 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OEEIJDKC_6 | 34578 | 35951 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_063856081.1 |
| OEEIJDKC_9 | 98331 | 99248 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| OEEIJDKC_9 | 411942 | 413582 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| OEEIJDKC_8 | 561058 | 561909 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| OEEIJDKC_6 | 37964 | 38482 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OEEIJDKC_6 | 518941 | 520122 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| OEEIJDKC_6 | 938328 | 945464 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391076 |
| OEEIJDKC_8 | 170781 | 171422 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| OEEIJDKC_6 | 933687 | 934472 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| OEEIJDKC_6 | 937370 | 938308 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| OEEIJDKC_6 | 934498 | 935694 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| OEEIJDKC_8 | 25214 | 26977 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| OEEIJDKC_6 | 935723 | 937342 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.07 | 100.00 | NP_391078 |
| OEEIJDKC_6 | 520605 | 521747 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| OEEIJDKC_6 | 520137 | 520586 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OEEIJDKC_1 | 31715 | 32851 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| OEEIJDKC_4 | 4341 | 5210 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| OEEIJDKC_7 | 308371 | 307595 | CodY | 8.49e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OEEIJDKC_6 | 681477 | 680908 | ClpP | 6.30e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| OEEIJDKC_7 | 770693 | 770301 | spxA1 | 6.67e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| OEEIJDKC_7 | 770693 | 770301 | spx | 3.98e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| OEEIJDKC_2 | 34652 | 34410 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| OEEIJDKC_9 | 362382 | 362579 | CspA | 1.91e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OEEIJDKC_1 | 0.0 | 1.0 | 0.0 |
| OEEIJDKC_2 | 0.0 | 1.0 | 0.0 |
| OEEIJDKC_3 | 0.0 | 1.0 | 0.0 |
| OEEIJDKC_4 | 0.0 | 1.0 | 0.0 |
| OEEIJDKC_5 | 0.0 | 1.0 | 0.0 |
| OEEIJDKC_6 | 0.0 | 1.0 | 0.0 |
| OEEIJDKC_7 | 0.0 | 1.0 | 0.0 |
| OEEIJDKC_8 | 0.0 | 1.0 | 0.0 |
| OEEIJDKC_9 | 0.0 | 1.0 | 0.0 |
| OEEIJDKC_10 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OEEIJDKC_3 | 5335 | 34225 | False | Myoviridae | VOG1309, VOG11060, VOG0189, VOG4693, VOG4673, VOG0796, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| OEEIJDKC_7 | 648004 | 675877 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)