Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PLOJDAAJ_36 | 97549 | 100119 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| PLOJDAAJ_58 | 87659 | 88828 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| PLOJDAAJ_6 | 56262 | 56600 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| PLOJDAAJ_6 | 55945 | 56262 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| PLOJDAAJ_48 | 45990 | 47636 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| PLOJDAAJ_2 | 43913 | 45352 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| PLOJDAAJ_2 | 31099 | 32019 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| PLOJDAAJ_13 | 20091 | 20684 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| PLOJDAAJ_121 | 8153 | 9355 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| PLOJDAAJ_75 | 6072 | 6926 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| PLOJDAAJ_2 | 31099 | 32019 | mph(K) | Spiramycin, Telithromycin | 99.24 | 100.00 | NC_000964 |
| PLOJDAAJ_75 | 6072 | 6926 | aadK | Streptomycin | 98.48 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PLOJDAAJ_48 | 45993 | 47636 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| PLOJDAAJ_2 | 31099 | 32016 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| PLOJDAAJ_75 | 6075 | 6926 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| PLOJDAAJ_37 | 4317 | 4835 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
| PLOJDAAJ_88 | 2 | 2452 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.54 | 94.35 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| PLOJDAAJ_1 | 109917 | 111098 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| PLOJDAAJ_71 | 76736 | 77377 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| PLOJDAAJ_22 | 111780 | 112976 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.42 | 100.00 | NP_391079 |
| PLOJDAAJ_22 | 113002 | 113787 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| PLOJDAAJ_82 | 6624 | 8387 | dep/capD | 0.0 | Capsule | Immune modulation | 99.09 | 100.00 | NP_389723 |
| PLOJDAAJ_1 | 111581 | 112723 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| PLOJDAAJ_22 | 102010 | 109146 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.70 | 100.00 | NP_391076 |
| PLOJDAAJ_1 | 111113 | 111562 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| PLOJDAAJ_22 | 109166 | 110104 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.62 | 100.00 | NP_391077 |
| PLOJDAAJ_22 | 110132 | 111751 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| PLOJDAAJ_45 | 28044 | 26908 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| PLOJDAAJ_98 | 3306 | 2437 | pdxS | 4.72e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| PLOJDAAJ_67 | 41132 | 40356 | CodY | 7.62e-126 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| PLOJDAAJ_1 | 267646 | 267077 | ClpP | 2.69e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| PLOJDAAJ_28 | 37350 | 36958 | spxA1 | 2.88e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| PLOJDAAJ_28 | 37350 | 36958 | spx | 1.72e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| PLOJDAAJ_5 | 20549 | 20791 | SpoVG | 4.74e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| PLOJDAAJ_50 | 21290 | 21487 | CspA | 9.35e-33 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| PLOJDAAJ_71 | 65505 | 65311 | CspD | 2.33e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| PLOJDAAJ_71 | 65505 | 65308 | CspB | 4.84e-28 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| PLOJDAAJ_1 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_2 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_3 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_4 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_5 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_6 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_7 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_8 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_11 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_12 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_13 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_15 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_17 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_18 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_19 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_21 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_22 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_23 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_24 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_25 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_27 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_28 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_29 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_30 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_31 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_32 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_33 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_35 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_36 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_37 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_38 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_39 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_40 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_41 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_42 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_43 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_44 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_45 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_46 | 1.0 | 0.0 | 1.0 |
| PLOJDAAJ_47 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_48 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_49 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_50 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_51 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_52 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_53 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_54 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_55 | 1.0 | 0.0 | 1.0 |
| PLOJDAAJ_56 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_57 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_58 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_59 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_61 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_62 | 1.0 | 0.0 | 1.0 |
| PLOJDAAJ_63 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_64 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_65 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_66 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_67 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_68 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_69 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_70 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_71 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_72 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_73 | 1.0 | 0.0 | 1.0 |
| PLOJDAAJ_74 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_75 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_76 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_78 | 1.0 | 0.0 | 1.0 |
| PLOJDAAJ_79 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_80 | 1.0 | 0.0 | 1.0 |
| PLOJDAAJ_81 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_82 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_85 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_87 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_88 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_89 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_90 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_91 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_92 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_93 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_94 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_95 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_97 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_98 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_99 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_100 | 1.0 | 0.0 | 1.0 |
| PLOJDAAJ_101 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_102 | 1.0 | 0.0 | 1.0 |
| PLOJDAAJ_105 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_108 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_111 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_112 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_113 | 1.0 | 0.0 | 1.0 |
| PLOJDAAJ_114 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_115 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_116 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_118 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_119 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_121 | 0.0 | 1.0 | 0.0 |
| PLOJDAAJ_123 | 1.0 | 0.0 | 1.0 |
| PLOJDAAJ_124 | 1.0 | 0.0 | 1.0 |
| PLOJDAAJ_125 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| PLOJDAAJ_6 | 85839 | 92914 | False | Myoviridae | VOG0136, VOG0753, VOG7895, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885 |
| PLOJDAAJ_30 | 54000 | 64328 | False | Siphoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749 |
Phigaro v2.4.0 (Database: Jan. 2024 release)