Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 2 | 7 | 7.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PLCCKAFL_24 | 39248 | 40297 | clbA | ARO:3002814 | clbA | 97.42 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| PLCCKAFL_24 | 39262 | 40297 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.03 | 98.67 | KR610408 |
| PLCCKAFL_35 | 28482 | 29858 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 87.15 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PLCCKAFL_24 | 39254 | 40297 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 97.70 | 99.71 | WP_012116915.1 |
| PLCCKAFL_35 | 28482 | 29852 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.21 | 99.78 | WP_003242953.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| PLCCKAFL_19 | 19999 | 21180 | capB | 0.0 | Capsule | Immune modulation | 82.29 | 99.66 | NP_391471 |
| PLCCKAFL_19 | 21195 | 21644 | capC | 8.48e-115 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| PLCCKAFL_14 | 31670 | 32806 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| PLCCKAFL_15 | 4301 | 5170 | pdxS | 6.87e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| PLCCKAFL_18 | 117646 | 118422 | CodY | 2.49e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| PLCCKAFL_21 | 156357 | 156749 | spxA1 | 4.18e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| PLCCKAFL_24 | 70976 | 70779 | CspA | 4.62e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| PLCCKAFL_22 | 135349 | 135155 | CspD | 3.66e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| PLCCKAFL_22 | 135349 | 135152 | CspB | 7.58e-28 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| PLCCKAFL_24 | 70976 | 70782 | CspR | 6.05e-27 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| PLCCKAFL_34 | 16987 | 16115 | lipA (SAUSA300_0829) | 5.08e-163 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| PLCCKAFL_1 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_4 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_5 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_7 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_8 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_9 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_10 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_11 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_12 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_13 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_14 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_15 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_16 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_17 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_18 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_19 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_20 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_21 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_22 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_23 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_24 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_25 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_26 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_27 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_28 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_29 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_30 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_31 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_32 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_33 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_34 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_35 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_36 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_37 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_38 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_39 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_41 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_42 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_43 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_44 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_45 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_46 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_47 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_48 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_50 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_51 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_53 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_54 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_56 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_57 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_58 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_60 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_62 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_64 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_65 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_66 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_67 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_68 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_69 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_70 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_74 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_76 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_78 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_80 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_81 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_82 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_83 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_86 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_87 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_89 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_90 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_92 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_93 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_94 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_95 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_97 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_98 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_99 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_100 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_102 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_103 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_105 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_106 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_107 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_110 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_112 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_116 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_120 | 1.0 | 0.0 | 1.0 |
| PLCCKAFL_122 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_123 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_124 | 0.0 | 1.0 | 0.0 |
| PLCCKAFL_128 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| PLCCKAFL_22 | 92622 | 117924 | False | Siphoviridae | VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641, VOG10230 |
| PLCCKAFL_30 | 3929 | 11588 | False | Myoviridae | VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
| PLCCKAFL_33 | 15719 | 26736 | False | Myoviridae / Siphoviridae | VOG9667, VOG5216, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG4870 |
| PLCCKAFL_95 | 10072 | 32279 | False | Siphoviridae | VOG4707, VOG0641, VOG7896, VOG3390, VOG4612, VOG4660, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG1266, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581 |
Phigaro v2.4.0 (Database: Jan. 2024 release)