safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	29	44.69

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
EGFLEGIP_1	886760	887599	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
EGFLEGIP_1	1412705	1413556	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
EGFLEGIP_1	1597500	1598261	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
EGFLEGIP_1	1755021	1755944	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
EGFLEGIP_1	1754111	1755061	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
EGFLEGIP_1	1753004	1754086	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
EGFLEGIP_1	1751940	1752914	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
EGFLEGIP_1	1751455	1751934	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
EGFLEGIP_1	1750144	1751262	STR_RS05230	0.0	Capsule	Immune modulation	100.00	100.00	WP_148148887
EGFLEGIP_1	1747854	1748453	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
EGFLEGIP_1	1745526	1746257	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
EGFLEGIP_1	1633025	1633618	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
EGFLEGIP_1	1424289	1426034	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
EGFLEGIP_1	1422587	1424287	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
EGFLEGIP_1	1420561	1421367	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
EGFLEGIP_1	1419620	1420561	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
EGFLEGIP_1	1418475	1419623	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
EGFLEGIP_1	75942	77600	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
EGFLEGIP_1	1755952	1757373	STR_RS05200	0.0	Capsule	Immune modulation	99.93	100.00	WP_011227236
EGFLEGIP_1	1744071	1745525	STR_RS05265	0.0	Capsule	Immune modulation	99.93	100.00	WP_041827043
EGFLEGIP_1	1759067	1760125	waaB	0.0	Capsule	Immune modulation	99.91	100.00	WP_157822584
EGFLEGIP_1	1748617	1749576	STR_RS05240	0.0	Capsule	Immune modulation	99.90	100.00	WP_041827042
EGFLEGIP_1	1746968	1747717	STR_RS05250	0.0	Capsule	Immune modulation	99.87	100.00	WP_011227246
EGFLEGIP_1	1746266	1746958	STR_RS05255	0.0	Capsule	Immune modulation	99.86	100.00	WP_011227247
EGFLEGIP_1	1633025	1633618	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
EGFLEGIP_1	1421367	1422569	STR_RS06895	0.0	Capsule	Immune modulation	99.83	100.00	WP_011227428
EGFLEGIP_1	1633025	1633618	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
EGFLEGIP_1	1422587	1424287	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
EGFLEGIP_1	1421367	1422569	STER_RS07055	0.0	Capsule	Immune modulation	99.58	100.00	WP_011681436
EGFLEGIP_1	1746266	1746958	STU_RS14640	0.0	Capsule	Immune modulation	99.57	100.00	WP_011226047
EGFLEGIP_1	1420561	1421367	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
EGFLEGIP_1	1419620	1420561	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
EGFLEGIP_1	1678882	1679672	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.37	100.00	-
EGFLEGIP_1	1418475	1419623	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
EGFLEGIP_1	1632156	1633025	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
EGFLEGIP_1	1745526	1746257	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
EGFLEGIP_1	1746968	1747703	STER_RS05305	0.0	Capsule	Immune modulation	98.37	99.33	WP_011681174
EGFLEGIP_1	1745526	1746257	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
EGFLEGIP_1	1424289	1426034	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
EGFLEGIP_1	1744071	1745525	STU_RS14650	0.0	Capsule	Immune modulation	98.01	100.00	WP_011226049
EGFLEGIP_1	1746968	1747703	STU_RS14635	0.0	Capsule	Immune modulation	97.83	99.33	WP_011226046
EGFLEGIP_1	1413677	1414936	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
EGFLEGIP_1	1744071	1745525	STER_RS05320	0.0	Capsule	Immune modulation	96.99	99.79	WP_011681177
EGFLEGIP_1	1746266	1746958	STER_RS05310	0.0	Capsule	Immune modulation	89.75	100.00	WP_011681175
EGFLEGIP_1	332898	334201	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
EGFLEGIP_1	1130840	1131850	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
EGFLEGIP_1	1420561	1421367	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
EGFLEGIP_1	780943	782226	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
EGFLEGIP_1	1634047	1635093	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
EGFLEGIP_1	1414933	1415631	STER_RS07095	2.91e-152	Capsule	Immune modulation	81.15	70.35	WP_011681444
EGFLEGIP_1	1749632	1749868	STR_RS05235*	6.58e-119	Capsule	Immune modulation	100.00	100.00	-
EGFLEGIP_1	1415985	1416451	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
EGFLEGIP_1	1421367	1422104	SGO_RS05025	0.0	Capsule	Immune modulation	85.09	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
EGFLEGIP_1	1594813	1593830	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
EGFLEGIP_1	223712	222639	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
EGFLEGIP_1	416437	415469	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
EGFLEGIP_1	1251372	1249069	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
EGFLEGIP_1	203188	200942	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
EGFLEGIP_1	1512327	1510846	SPD_0310	0.0	UPF0371 protein SPD_0310	91.90	100.00	ABJ54480
EGFLEGIP_1	867969	866977	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
EGFLEGIP_1	904081	903110	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
EGFLEGIP_1	1365202	1366353	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
EGFLEGIP_1	1399675	1401657	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
EGFLEGIP_1	1611007	1612737	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
EGFLEGIP_1	942284	943762	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
EGFLEGIP_1	334201	332900	Eno	0.0	enolase	86.21	99.77	ABP90468
EGFLEGIP_1	957021	958913	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
EGFLEGIP_1	198017	199726	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
EGFLEGIP_1	1090158	1089148	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
EGFLEGIP_1	50592	51953	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
EGFLEGIP_1	1039827	1041719	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
EGFLEGIP_1	348631	347741	bglB	2.81e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
EGFLEGIP_1	138617	139552	SP_0676	7.12e-168	transcription facot	81.73	96.89	AAK74821
EGFLEGIP_1	200750	199893	metF	8.34e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
EGFLEGIP_1	1707371	1708075	covR	3.38e-130	responder protein	84.68	100.00	AAK71319
EGFLEGIP_1	1541629	1542300	rr01	2.84e-125	response regulator	84.38	99.56	CAB54566
EGFLEGIP_1	1707371	1708075	walR	2.42e-120	DNA-binding response regulator	80.85	100.00	ANC99504
EGFLEGIP_1	629547	628861	CovR	9.70e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
EGFLEGIP_1	194064	193414	CiaR	1.34e-116	two-component response regulator	86.17	96.88	AAK74935
EGFLEGIP_1	194064	193414	ciaR	6.87e-114	two component system response regulator	83.87	96.02	AIK75708
EGFLEGIP_1	866680	866285	SpxA2	3.57e-74	transcriptional regulator	90.91	100.00	ERL21461
EGFLEGIP_1	39234	38833	SpxA1	3.63e-66	transcriptional regulator	80.60	100.00	ERL19273
EGFLEGIP_1	780785	780285	RopE (M5005_Spy_1611)	9.53e-75	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
EGFLEGIP_1	1251372	1249075	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
EGFLEGIP_1	1054288	1055307	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
EGFLEGIP_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found