Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 2 | 7 | 7.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AKBHBEKJ_23 | 373810 | 374859 | clbA | ARO:3002814 | clbA | 99.71 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| AKBHBEKJ_23 | 373810 | 374845 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 89.09 | 98.67 | KR610408 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AKBHBEKJ_23 | 373810 | 374856 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.71 | 100.00 | WP_012116915.1 |
| AKBHBEKJ_21 | 137319 | 139913 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.11 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AKBHBEKJ_10 | 434155 | 435336 | capB | 0.0 | Capsule | Immune modulation | 81.96 | 99.66 | NP_391471 |
| AKBHBEKJ_10 | 435351 | 435800 | capC | 8.00e-113 | Capsule | Immune modulation | 83.37 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AKBHBEKJ_25 | 27954 | 26818 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| AKBHBEKJ_7 | 4279 | 5148 | pdxS | 1.35e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| AKBHBEKJ_21 | 341095 | 340319 | CodY | 4.68e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| AKBHBEKJ_23 | 739084 | 739476 | spxA1 | 2.27e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| AKBHBEKJ_23 | 349585 | 349782 | CspA | 6.08e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| AKBHBEKJ_27 | 615236 | 615042 | CspD | 1.64e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| AKBHBEKJ_27 | 615236 | 615039 | CspB | 3.41e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| AKBHBEKJ_2 | 184175 | 185047 | lipA (SAUSA300_0829) | 1.06e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| AKBHBEKJ_11 | 7042 | 7228 | Col(SD853) | 187 / 194 | 86.63 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AKBHBEKJ_1 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_2 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_3 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_5 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_6 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_7 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_8 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_9 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_10 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_11 | 1.0 | 0.0 | 1.0 |
| AKBHBEKJ_14 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_16 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_17 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_18 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_19 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_21 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_22 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_23 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_24 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_25 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_27 | 0.0 | 1.0 | 0.0 |
| AKBHBEKJ_28 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AKBHBEKJ_23 | 911528 | 916967 | False | Siphoviridae | VOG9667, VOG0189, VOG4861, VOG5447, VOG4612, VOG3390 |
| AKBHBEKJ_27 | 560321 | 563005 | False | Unknown | VOG9667, VOG9667, VOG0650, VOG4552 |
| AKBHBEKJ_27 | 571036 | 597923 | False | Siphoviridae | VOG2703, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641, VOG10230, VOG3532 |
Phigaro v2.4.0 (Database: Jan. 2024 release)