Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 1 | 7 | 7.35 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FEDHANBN_41 | 51000 | 52049 | clbA | ARO:3002814 | clbA | 97.42 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FEDHANBN_41 | 51014 | 52049 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 87.74 | 98.67 | KR610408 |
| FEDHANBN_39 | 410653 | 412029 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 86.64 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FEDHANBN_41 | 51006 | 52049 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 97.70 | 99.71 | WP_012116915.1 |
| FEDHANBN_39 | 410653 | 412023 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FEDHANBN_12 | 492884 | 494065 | capB | 0.0 | Capsule | Immune modulation | 82.29 | 99.66 | NP_391471 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FEDHANBN_7 | 31665 | 32801 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| FEDHANBN_19 | 4256 | 5125 | pdxS | 8.69e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| FEDHANBN_40 | 339151 | 339927 | CodY | 7.88e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FEDHANBN_23 | 37698 | 37306 | spxA1 | 1.02e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| FEDHANBN_41 | 70430 | 70233 | CspA | 6.55e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| FEDHANBN_5 | 145491 | 145685 | CspD | 8.65e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| FEDHANBN_5 | 145491 | 145688 | CspB | 1.79e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| FEDHANBN_41 | 70430 | 70236 | CspR | 8.58e-27 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| FEDHANBN_12 | 849932 | 850804 | lipA (SAUSA300_0829) | 2.77e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FEDHANBN_1 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_3 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_4 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_5 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_7 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_8 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_9 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_10 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_11 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_12 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_13 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_14 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_15 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_16 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_17 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_18 | 1.0 | 0.0 | 1.0 |
| FEDHANBN_19 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_23 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_24 | 1.0 | 0.0 | 1.0 |
| FEDHANBN_25 | 1.0 | 0.0 | 1.0 |
| FEDHANBN_28 | 1.0 | 0.0 | 1.0 |
| FEDHANBN_32 | 1.0 | 0.0 | 1.0 |
| FEDHANBN_34 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_39 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_40 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_41 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_42 | 0.0 | 1.0 | 0.0 |
| FEDHANBN_43 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FEDHANBN_4 | 33293 | 58950 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
| FEDHANBN_39 | 280346 | 309143 | False | Siphoviridae | VOG1227, VOG4606, VOG2752, VOG7372, VOG0198, VOG0376, VOG2545, VOG4841, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG1266, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4660, VOG4612, VOG3390, VOG7896 |
Phigaro v2.4.0 (Database: Jan. 2024 release)