safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
AAAJLPDC_1	4078852	4079772	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
AAAJLPDC_1	4065524	4066957	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
AAAJLPDC_1	4015863	4016456	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
AAAJLPDC_1	3749268	3750911	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
AAAJLPDC_1	3434414	3436984	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
AAAJLPDC_1	2976818	2977156	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
AAAJLPDC_1	2976501	2976818	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
AAAJLPDC_1	1812312	1813481	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
AAAJLPDC_1	1589978	1591180	blt	ARO:3003006	blt	100.00	100.00	L32599.1
AAAJLPDC_1	1571623	1572477	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
AAAJLPDC_1	1571623	1572477	aadK	Streptomycin	100.00	100.00	M26879
AAAJLPDC_1	4078852	4079772	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
AAAJLPDC_1	119481	120857	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
AAAJLPDC_1	1571623	1572474	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
AAAJLPDC_1	4078855	4079772	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
AAAJLPDC_1	122857	123375	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
AAAJLPDC_1	119481	120854	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
AAAJLPDC_1	3749268	3750908	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
AAAJLPDC_1	2255858	2258452	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
AAAJLPDC_1	609167	610309	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
AAAJLPDC_1	607503	608684	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
AAAJLPDC_1	608699	609148	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
AAAJLPDC_1	2303343	2305106	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
AAAJLPDC_1	1020472	1027608	dhbF	Bacillibactin	Nutritional/Metabolic factor	99.99	100.00	NP_391076
AAAJLPDC_1	1019514	1020452	dhbB	Bacillibactin	Nutritional/Metabolic factor	99.89	100.00	NP_391077
AAAJLPDC_1	1015830	1016616	dhbA	Bacillibactin	Nutritional/Metabolic factor	99.87	100.00	NP_391080
AAAJLPDC_1	2012130	2012770	hlyIII	Hemolysin III	Exotoxin	99.84	99.84	NP_390062
AAAJLPDC_1	1016642	1017838	dhbC	Bacillibactin	Nutritional/Metabolic factor	99.67	100.00	NP_391079
AAAJLPDC_1	1017867	1019486	dhbE	Bacillibactin	Nutritional/Metabolic factor	99.57	100.00	NP_391078

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
AAAJLPDC_2	27922	26786	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
AAAJLPDC_1	71158	70289	pdxS	3.10e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
AAAJLPDC_1	2663568	2662792	CodY	3.39e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
AAAJLPDC_1	761902	761333	ClpP	2.51e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
AAAJLPDC_1	0.0	1.0	0.0
AAAJLPDC_2	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
AAAJLPDC_1	1610729	1641105	False	Myoviridae	VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718
AAAJLPDC_1	2434718	2459803	False	Siphoviridae	VOG4918, VOG0641, VOG5008, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG4772, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581
AAAJLPDC_1	3006995	3033103	False	Myoviridae	VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667

Phigaro v2.4.0 (Database: Jan. 2024 release)