Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 9 | 18.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IHBLKMDC_1 | 502757 | 503095 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| IHBLKMDC_1 | 502440 | 502757 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| IHBLKMDC_18 | 480524 | 481726 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| IHBLKMDC_21 | 475160 | 476329 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| IHBLKMDC_18 | 462211 | 463065 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| IHBLKMDC_22 | 403872 | 405515 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| IHBLKMDC_22 | 162744 | 163337 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| IHBLKMDC_20 | 117759 | 120329 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| IHBLKMDC_22 | 112266 | 113705 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| IHBLKMDC_22 | 99452 | 100372 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IHBLKMDC_5 | 53167 | 54543 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.84 | 100.00 | X08034 |
| IHBLKMDC_18 | 462211 | 463063 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| IHBLKMDC_22 | 99452 | 100372 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IHBLKMDC_5 | 53170 | 54543 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| IHBLKMDC_22 | 403875 | 405515 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| IHBLKMDC_18 | 462211 | 463062 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| IHBLKMDC_22 | 99452 | 100369 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| IHBLKMDC_5 | 50696 | 51214 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| IHBLKMDC_21 | 177567 | 180164 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IHBLKMDC_21 | 273713 | 274354 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| IHBLKMDC_12 | 7452 | 8390 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| IHBLKMDC_12 | 407321 | 408502 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IHBLKMDC_12 | 8418 | 10037 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| IHBLKMDC_12 | 11288 | 12073 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| IHBLKMDC_12 | 405696 | 406838 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| IHBLKMDC_12 | 10066 | 11262 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| IHBLKMDC_21 | 130626 | 132389 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| IHBLKMDC_12 | 296 | 7432 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.84 | 100.00 | NP_391076 |
| IHBLKMDC_12 | 406857 | 407306 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IHBLKMDC_7 | 27946 | 26810 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IHBLKMDC_11 | 4261 | 5130 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IHBLKMDC_1 | 192910 | 192134 | CodY | 5.39e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IHBLKMDC_12 | 265904 | 266473 | ClpP | 5.04e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| IHBLKMDC_1 | 654626 | 654234 | spxA1 | 4.23e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| IHBLKMDC_1 | 654626 | 654234 | spx | 2.53e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| IHBLKMDC_2 | 34778 | 34536 | SpoVG | 7.53e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| IHBLKMDC_22 | 357144 | 357341 | CspA | 1.88e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| IHBLKMDC_21 | 286317 | 286511 | CspD | 3.89e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| IHBLKMDC_19 | 33100 | 32906 | CspB | 2.45e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IHBLKMDC_1 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_2 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_4 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_5 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_7 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_9 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_10 | 1.0 | 0.0 | 1.0 |
| IHBLKMDC_11 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_12 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_14 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_15 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_16 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_17 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_18 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_19 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_20 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_21 | 0.0 | 1.0 | 0.0 |
| IHBLKMDC_22 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IHBLKMDC_1 | 532579 | 560062 | False | Myoviridae | VOG0753, VOG4705, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| IHBLKMDC_4 | 12351 | 21514 | False | Siphoviridae | VOG0275, VOG4602, VOG4662, VOG9667, VOG9667, VOG0650, VOG4552, VOG4600, VOG1309, VOG4566 |
| IHBLKMDC_4 | 26821 | 49590 | False | Siphoviridae | VOG0198, VOG7983, VOG0796, VOG10227, VOG0691, VOG0692, VOG9317, VOG4572, VOG2621, VOG0695, VOG0696, VOG0697, VOG0698, VOG10044, VOG0701, VOG9745, VOG4605, VOG4599, VOG5535, VOG10940, VOG0753, VOG0136, VOG4574, VOG2703, VOG9093 |
| IHBLKMDC_10 | 7419 | 15541 | False | Unknown | VOG1198, VOG0397, VOG2352, VOG11080 |
| IHBLKMDC_10 | 32423 | 57673 | False | Siphoviridae | VOG4615, VOG0703, VOG3616, VOG1024, VOG10944, VOG9244, VOG4599, VOG4605, VOG6163, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG1886, VOG5819, VOG4581, VOG0221 |
| IHBLKMDC_17 | 4376 | 36505 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG4567, VOG4673, VOG7060, VOG0796, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
Phigaro v2.4.0 (Database: Jan. 2024 release)