safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	27	43.42

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
FFJEJNBP_1	865148	865987	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
FFJEJNBP_1	1661804	1662594	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	100.00	100.00	-
FFJEJNBP_1	1390015	1390866	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
FFJEJNBP_1	1579177	1579938	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
FFJEJNBP_1	1737882	1738805	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
FFJEJNBP_1	1736972	1737922	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
FFJEJNBP_1	1735865	1736947	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
FFJEJNBP_1	1734801	1735775	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
FFJEJNBP_1	1734316	1734795	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
FFJEJNBP_1	1733005	1734123	STR_RS05230	0.0	Capsule	Immune modulation	100.00	100.00	WP_148148887
FFJEJNBP_1	1731478	1732437	STR_RS05240	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827042
FFJEJNBP_1	1730715	1731314	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
FFJEJNBP_1	1729829	1730578	STR_RS05250	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227246
FFJEJNBP_1	1729127	1729819	STR_RS05255	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227247
FFJEJNBP_1	1728387	1729118	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
FFJEJNBP_1	1726932	1728386	STR_RS05265	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827043
FFJEJNBP_1	1615947	1616540	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
FFJEJNBP_1	1401599	1403344	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
FFJEJNBP_1	1398677	1399879	STR_RS06895	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227428
FFJEJNBP_1	1397871	1398677	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
FFJEJNBP_1	1396930	1397871	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
FFJEJNBP_1	1395785	1396933	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
FFJEJNBP_1	1741928	1742986	waaB	0.0	Capsule	Immune modulation	100.00	100.00	WP_157822584
FFJEJNBP_1	51013	52671	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
FFJEJNBP_1	1399897	1401597	STR_RS06890	0.0	Capsule	Immune modulation	99.94	100.00	WP_041827077
FFJEJNBP_1	1738813	1740234	STR_RS05200	0.0	Capsule	Immune modulation	99.93	100.00	WP_011227236
FFJEJNBP_1	1615947	1616540	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
FFJEJNBP_1	1615947	1616540	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
FFJEJNBP_1	1399897	1401597	STER_RS07050	0.0	Capsule	Immune modulation	99.65	100.00	WP_041827753
FFJEJNBP_1	1397871	1398677	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
FFJEJNBP_1	1396930	1397871	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
FFJEJNBP_1	1398677	1399879	STER_RS07055	0.0	Capsule	Immune modulation	99.42	100.00	WP_011681436
FFJEJNBP_1	1729127	1729819	STU_RS14640	0.0	Capsule	Immune modulation	99.42	100.00	WP_011226047
FFJEJNBP_1	1395785	1396933	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
FFJEJNBP_1	1615078	1615947	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
FFJEJNBP_1	1728387	1729118	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
FFJEJNBP_1	1729829	1730564	STER_RS05305	0.0	Capsule	Immune modulation	98.50	99.33	WP_011681174
FFJEJNBP_1	1728387	1729118	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
FFJEJNBP_1	1401599	1403344	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
FFJEJNBP_1	1729829	1730564	STU_RS14635	0.0	Capsule	Immune modulation	97.96	99.33	WP_011226046
FFJEJNBP_1	1726932	1728386	STU_RS14650	0.0	Capsule	Immune modulation	97.94	100.00	WP_011226049
FFJEJNBP_1	1390987	1392246	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
FFJEJNBP_1	1726932	1728386	STER_RS05320	0.0	Capsule	Immune modulation	96.92	99.79	WP_011681177
FFJEJNBP_1	1729127	1729819	STER_RS05310	0.0	Capsule	Immune modulation	89.61	100.00	WP_011681175
FFJEJNBP_1	318377	319680	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
FFJEJNBP_1	1109283	1110293	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
FFJEJNBP_1	1397871	1398677	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
FFJEJNBP_1	765136	766419	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
FFJEJNBP_1	1616969	1618015	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
FFJEJNBP_1	1392243	1392941	STER_RS07095	2.92e-152	Capsule	Immune modulation	81.15	70.35	WP_011681444
FFJEJNBP_1	1732493	1732729	STR_RS05235*	6.59e-119	Capsule	Immune modulation	100.00	100.00	-
FFJEJNBP_1	1393295	1393761	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
FFJEJNBP_1	1398677	1399414	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
FFJEJNBP_1	1576490	1575507	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
FFJEJNBP_1	207107	206034	MntE	0.0	manganese homeostasis	94.13	99.72	ABF32167
FFJEJNBP_1	401916	400948	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
FFJEJNBP_1	1228681	1226378	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
FFJEJNBP_1	1489629	1488148	SPD_0310	0.0	UPF0371 protein SPD_0310	92.11	100.00	ABJ54480
FFJEJNBP_1	178426	176180	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
FFJEJNBP_1	846356	845364	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
FFJEJNBP_1	882469	881498	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
FFJEJNBP_1	1342512	1343663	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
FFJEJNBP_1	1376985	1378967	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
FFJEJNBP_1	1592684	1594414	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.69	100.00	AAK34199
FFJEJNBP_1	920729	922207	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
FFJEJNBP_1	319680	318379	Eno	0.0	enolase	86.21	99.77	ABP90468
FFJEJNBP_1	935465	937357	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
FFJEJNBP_1	173255	174964	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
FFJEJNBP_1	1068602	1067592	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
FFJEJNBP_1	1018271	1020163	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
FFJEJNBP_1	334110	333220	bglB	2.82e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
FFJEJNBP_1	113856	114791	SP_0676	7.14e-168	transcription facot	81.73	96.89	AAK74821
FFJEJNBP_1	175988	175131	metF	8.36e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
FFJEJNBP_1	1690294	1690998	covR	3.39e-130	responder protein	84.68	100.00	AAK71319
FFJEJNBP_1	1523308	1523979	rr01	1.16e-125	response regulator	84.38	99.56	CAB54566
FFJEJNBP_1	1690294	1690998	walR	2.43e-120	DNA-binding response regulator	80.85	100.00	ANC99504
FFJEJNBP_1	614992	614306	CovR	9.73e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
FFJEJNBP_1	169302	168652	CiaR	1.34e-116	two-component response regulator	86.17	96.88	AAK74935
FFJEJNBP_1	169302	168652	ciaR	6.89e-114	two component system response regulator	83.87	96.02	AIK75708
FFJEJNBP_1	845067	844672	SpxA2	3.58e-74	transcriptional regulator	90.91	100.00	ERL21461
FFJEJNBP_1	764978	764478	RopE (M5005_Spy_1611)	9.55e-75	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
FFJEJNBP_1	1228681	1226384	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
FFJEJNBP_1	1032732	1033751	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
FFJEJNBP_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found