Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 7 | 8.31 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCCBHEMI_35 | 23544 | 24593 | clbA | ARO:3002814 | clbA | 92.84 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MCCBHEMI_35 | 23544 | 24593 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 89.06 | 99.90 | KR610408 |
| MCCBHEMI_31 | 375567 | 376943 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 87.22 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCCBHEMI_35 | 23544 | 24590 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 92.84 | 100.00 | WP_012116915.1 |
| MCCBHEMI_31 | 375567 | 376937 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 87.31 | 99.78 | WP_003242953.1 |
| MCCBHEMI_35 | 21281 | 21799 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 82.66 | 100.00 | WP_003242546.1 |
| MCCBHEMI_1 | 250247 | 252844 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MCCBHEMI_23 | 497699 | 498880 | capB | 0.0 | Capsule | Immune modulation | 82.12 | 99.66 | NP_391471 |
| MCCBHEMI_23 | 497235 | 497684 | capC | 1.18e-112 | Capsule | Immune modulation | 83.37 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MCCBHEMI_8 | 27580 | 26444 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| MCCBHEMI_17 | 4470 | 5339 | pdxS | 6.49e-153 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| MCCBHEMI_1 | 450841 | 450065 | CodY | 6.96e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MCCBHEMI_12 | 102471 | 102079 | spxA1 | 4.15e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| MCCBHEMI_35 | 5560 | 5757 | CspA | 7.43e-33 | cold shock protein | 92.42 | 100.00 | CAA62903 |
| MCCBHEMI_20 | 39910 | 39716 | CspB | 9.23e-28 | cold shock protein | 84.61 | 98.48 | CAD00094 |
| MCCBHEMI_35 | 5560 | 5754 | CspR | 3.68e-27 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| MCCBHEMI_23 | 136675 | 135809 | lipA (SAUSA300_0829) | 1.30e-161 | lipoic acid synthetase | 81.03 | 94.75 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| MCCBHEMI_3 | 4853 | 5046 | Col(SD853) | 194 / 194 | 97.94 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MCCBHEMI_1 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_3 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_4 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_5 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_6 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_8 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_9 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_10 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_11 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_12 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_14 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_15 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_16 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_17 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_18 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_19 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_20 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_21 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_22 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_23 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_24 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_25 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_26 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_27 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_28 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_30 | 1.0 | 0.0 | 1.0 |
| MCCBHEMI_31 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_32 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_33 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_34 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_35 | 0.0 | 1.0 | 0.0 |
| MCCBHEMI_36 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MCCBHEMI_1 | 82831 | 104204 | False | Siphoviridae | VOG4556, VOG4581, VOG4544, VOG5051, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG5852, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5221, VOG0136 |
| MCCBHEMI_1 | 830378 | 838434 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885 |
| MCCBHEMI_12 | 4907 | 14425 | False | Siphoviridae | VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
| MCCBHEMI_31 | 250143 | 276191 | False | Siphoviridae | VOG0376, VOG2545, VOG0531, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641, VOG4707 |
Phigaro v2.4.0 (Database: Jan. 2024 release)