safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
FNKCPBGA_1	4001462	4003105	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
FNKCPBGA_1	3688496	3691066	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
FNKCPBGA_1	3230988	3231326	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
FNKCPBGA_1	3230671	3230988	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
FNKCPBGA_1	2116390	2117559	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
FNKCPBGA_1	1893962	1895164	blt	ARO:3003006	blt	100.00	100.00	L32599.1
FNKCPBGA_1	1875601	1876455	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
FNKCPBGA_1	119738	120658	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
FNKCPBGA_1	106410	107843	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
FNKCPBGA_1	56747	57340	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
FNKCPBGA_1	1875601	1876455	aadK	Streptomycin	100.00	100.00	M26879
FNKCPBGA_1	119738	120658	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
FNKCPBGA_1	423053	424429	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
FNKCPBGA_1	1875601	1876452	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
FNKCPBGA_1	119741	120658	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
FNKCPBGA_1	426429	426947	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
FNKCPBGA_1	423053	424426	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
FNKCPBGA_1	4001462	4003102	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
FNKCPBGA_1	2558279	2560873	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
FNKCPBGA_1	913147	914289	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
FNKCPBGA_1	911483	912664	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
FNKCPBGA_1	912679	913128	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
FNKCPBGA_1	2605768	2607531	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
FNKCPBGA_1	1319840	1320625	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
FNKCPBGA_1	1323523	1324461	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
FNKCPBGA_1	1320651	1321847	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
FNKCPBGA_1	1321876	1323495	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
FNKCPBGA_1	1324481	1331617	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
FNKCPBGA_1	2316264	2316905	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
FNKCPBGA_1	263622	262486	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
FNKCPBGA_1	377430	376561	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
FNKCPBGA_1	2917724	2916948	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
FNKCPBGA_1	1065901	1065332	ClpP	2.53e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
FNKCPBGA_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
FNKCPBGA_1	1914716	1945093	False	Myoviridae	VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718
FNKCPBGA_1	3261165	3287273	False	Myoviridae	VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667

Phigaro v2.4.0 (Database: Jan. 2024 release)