safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
2	1	0	2.24

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
JKFIHHCI_131	40487	41389	CepA-44	ARO:3006225	CepA-44	99.33	100.00	NG_050386.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
JKFIHHCI_87	1	656	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	100.00	81.90	M17808
JKFIHHCI_131	40487	41389	cepA	Unknown Beta-lactam	99.89	100.00	U05887

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
JKFIHHCI_87	1	654	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	PARTIAL_CONTIG_ENDX	100.00	81.95	WP_002682030.1
JKFIHHCI_131	40490	41389	cepA	CepA family extended-spectrum class A beta-lactamase	BLASTX	99.67	100.00	WP_005816369.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
JKFIHHCI_86	314461	315654	bft-2	BFT B. fragilis toxin, subtype 2	Exotoxin	100.00	100.00	BAA77277
JKFIHHCI_86	314461	315654	bft-3	BFT B. fragilis toxin, subtype 3	Exotoxin	96.90	100.00	BAA77275
JKFIHHCI_86	314461	315654	bft-1	BFT B. fragilis toxin, subtype 1	Exotoxin	94.89	100.00	BAA77276

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
JKFIHHCI_78	641	771	ColRNAI	131 / 130	87.02	DQ298019

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
JKFIHHCI_1	1.0	0.0	1.0
JKFIHHCI_7	0.0	1.0	0.0
JKFIHHCI_17	0.0	1.0	0.0
JKFIHHCI_25	0.0	1.0	0.0
JKFIHHCI_34	0.0	1.0	0.0
JKFIHHCI_42	0.0	1.0	0.0
JKFIHHCI_53	0.0	1.0	0.0
JKFIHHCI_61	0.0	1.0	0.0
JKFIHHCI_64	0.0	1.0	0.0
JKFIHHCI_65	0.0	1.0	0.0
JKFIHHCI_66	0.0	1.0	0.0
JKFIHHCI_67	0.0	1.0	0.0
JKFIHHCI_68	0.0	1.0	0.0
JKFIHHCI_69	0.0	1.0	0.0
JKFIHHCI_70	0.0	1.0	0.0
JKFIHHCI_71	0.0	1.0	0.0
JKFIHHCI_72	0.0	1.0	0.0
JKFIHHCI_73	1.0	0.0	1.0
JKFIHHCI_74	1.0	0.0	1.0
JKFIHHCI_76	0.0	1.0	0.0
JKFIHHCI_77	1.0	0.0	1.0
JKFIHHCI_79	1.0	0.0	1.0
JKFIHHCI_86	0.0	1.0	0.0
JKFIHHCI_97	0.0	1.0	0.0
JKFIHHCI_107	0.0	1.0	0.0
JKFIHHCI_116	0.0	1.0	0.0
JKFIHHCI_125	0.0	1.0	0.0
JKFIHHCI_131	0.0	1.0	0.0
JKFIHHCI_139	0.0	1.0	0.0
JKFIHHCI_140	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found

Phigaro v2.4.0 (Database: Jan. 2024 release)