Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CBGBCKBI_1 | 2682350 | 2683204 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| CBGBCKBI_1 | 2663658 | 2664860 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| CBGBCKBI_1 | 2467018 | 2468187 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| CBGBCKBI_1 | 1354991 | 1355308 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| CBGBCKBI_1 | 1354653 | 1354991 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| CBGBCKBI_1 | 894918 | 897488 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| CBGBCKBI_1 | 583269 | 584912 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| CBGBCKBI_1 | 338746 | 339339 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| CBGBCKBI_1 | 288247 | 289680 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| CBGBCKBI_1 | 275432 | 276352 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| CBGBCKBI_1 | 275432 | 276352 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| CBGBCKBI_1 | 4138670 | 4140046 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
| CBGBCKBI_1 | 2682352 | 2683204 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CBGBCKBI_1 | 275432 | 276349 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| CBGBCKBI_1 | 4138673 | 4140046 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| CBGBCKBI_1 | 583272 | 584912 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| CBGBCKBI_1 | 2682353 | 2683204 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| CBGBCKBI_1 | 4136153 | 4136671 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 89.60 | 100.00 | WP_003242546.1 |
| CBGBCKBI_1 | 2029504 | 2032101 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.37 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| CBGBCKBI_1 | 3647360 | 3648541 | capB | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | NP_391471 |
| CBGBCKBI_1 | 1979214 | 1980977 | dep/capD | 0.0 | Capsule | Immune modulation | 99.77 | 100.00 | NP_389723 |
| CBGBCKBI_1 | 3235606 | 3237225 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.69 | 100.00 | NP_391078 |
| CBGBCKBI_1 | 3234640 | 3235578 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391077 |
| CBGBCKBI_1 | 3238476 | 3239261 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| CBGBCKBI_1 | 3646896 | 3647345 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| CBGBCKBI_1 | 3227484 | 3234620 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391076 |
| CBGBCKBI_1 | 2267668 | 2268309 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| CBGBCKBI_1 | 3645736 | 3646877 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 99.91 | NP_391469 |
| CBGBCKBI_1 | 3237254 | 3238450 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| CBGBCKBI_1 | 132488 | 133624 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| CBGBCKBI_1 | 18758 | 19627 | pdxS | 3.09e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| CBGBCKBI_1 | 1664862 | 1665638 | CodY | 3.38e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| CBGBCKBI_1 | 3494142 | 3494711 | ClpP | 2.50e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| CBGBCKBI_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| CBGBCKBI_1 | 1297147 | 1324805 | False | Myoviridae | VOG0101, VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)