Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 8 | 4 | 13.42 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PNKNBELK_1 | 2059039 | 2059821 | ANT(9)-Ia | ARO:3002630 | ANT(9)-Ia | 100.00 | 100.00 | X02588.1 |
| PNKNBELK_1 | 1376859 | 1377176 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| PNKNBELK_1 | 1376521 | 1376859 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| PNKNBELK_1 | 916779 | 919349 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| PNKNBELK_1 | 604736 | 606379 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| PNKNBELK_1 | 339155 | 339748 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| PNKNBELK_1 | 288652 | 290085 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| PNKNBELK_1 | 275837 | 276757 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| PNKNBELK_1 | 2059039 | 2059821 | ant(9)-Ia | Spectinomycin | 100.00 | 100.00 | X02588 |
| PNKNBELK_1 | 275837 | 276757 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| PNKNBELK_1 | 3203259 | 3204635 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PNKNBELK_1 | 2059042 | 2059821 | ant(9)-Ia | aminoglycoside nucleotidyltransferase ANT(9)-Ia | EXACTX | 100.00 | 100.00 | WP_000067268.1 |
| PNKNBELK_1 | 275837 | 276754 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| PNKNBELK_1 | 3200741 | 3201259 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| PNKNBELK_1 | 3203262 | 3204635 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| PNKNBELK_1 | 604739 | 606379 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| PNKNBELK_1 | 2713398 | 2714540 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| PNKNBELK_1 | 2715023 | 2716204 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| PNKNBELK_1 | 2714559 | 2715008 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| PNKNBELK_1 | 2307088 | 2307873 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| PNKNBELK_1 | 2303252 | 2304190 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391077 |
| PNKNBELK_1 | 2305866 | 2307062 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391079 |
| PNKNBELK_1 | 2296096 | 2303232 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391076 |
| PNKNBELK_1 | 2304218 | 2305837 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.32 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| PNKNBELK_1 | 132882 | 134018 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| PNKNBELK_1 | 19074 | 19943 | pdxS | 3.13e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| PNKNBELK_1 | 1690123 | 1690899 | CodY | 3.42e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| PNKNBELK_1 | 2561811 | 2562380 | ClpP | 2.54e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| PNKNBELK_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| PNKNBELK_1 | 1320574 | 1347004 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)