safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	29	38.29

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
FGFKEIIK_1	1540500	1541093	STER_RS06030	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
FGFKEIIK_1	1665950	1667410	STER_RS05320	0.0	Capsule	Immune modulation	99.93	100.00	WP_011681177
FGFKEIIK_1	1540500	1541093	STR_RS05880	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
FGFKEIIK_1	1327638	1328447	STU_RS16245	0.0	Capsule	Immune modulation	99.75	100.00	WP_002948020
FGFKEIIK_1	1539631	1540500	rfbA	0.0	Capsule	Immune modulation	99.66	100.00	WP_011681286
FGFKEIIK_1	1540500	1541093	STU_RS15260	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
FGFKEIIK_1	1784394	1786052	fbp54	0.0	FBPs	Adherence	99.64	100.00	WP_011227179
FGFKEIIK_1	1326698	1327654	STU_RS16250	0.0	Capsule	Immune modulation	99.58	100.00	WP_011226328
FGFKEIIK_1	771598	772437	cbpD	0.0	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
FGFKEIIK_1	1330736	1332520	STU_RS16230	0.0	Capsule	Immune modulation	99.44	100.00	WP_011226325
FGFKEIIK_1	1328447	1329652	STU_RS16240	0.0	Capsule	Immune modulation	99.17	100.00	WP_011226326
FGFKEIIK_1	1319660	1320511	rfbD	0.0	Capsule	Immune modulation	99.06	100.00	WP_014621820
FGFKEIIK_1	1667411	1668142	STER_RS05315	0.0	Capsule	Immune modulation	98.91	100.00	WP_011681176
FGFKEIIK_1	1503546	1504307	srtA	0.0	Sortase A	Exoenzyme	98.82	100.00	-
FGFKEIIK_1	1325553	1326701	STU_RS16255	0.0	Capsule	Immune modulation	98.78	100.00	WP_041828290
FGFKEIIK_1	1667411	1668142	STR_RS05260	0.0	Capsule	Immune modulation	98.50	100.00	WP_011227248
FGFKEIIK_1	1667411	1668142	STU_RS14645	0.0	Capsule	Immune modulation	98.36	100.00	WP_011226048
FGFKEIIK_1	1599275	1600068	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	98.24	100.25	-
FGFKEIIK_1	1678159	1679574	STER_RS05250	0.0	Capsule	Immune modulation	98.23	100.00	WP_011681165
FGFKEIIK_1	1668853	1669593	STU_RS14635	0.0	Capsule	Immune modulation	98.11	100.00	WP_011226046
FGFKEIIK_1	1668853	1669593	STER_RS05305	0.0	Capsule	Immune modulation	97.71	100.00	WP_011681174
FGFKEIIK_1	1665950	1667410	STU_RS14650	0.0	Capsule	Immune modulation	97.40	100.21	WP_011226049
FGFKEIIK_1	1668853	1669588	STR_RS05250	0.0	Capsule	Immune modulation	97.15	98.13	WP_011227246
FGFKEIIK_1	1669650	1671017	STU_RS14630	0.0	Capsule	Immune modulation	97.00	100.00	WP_011226045
FGFKEIIK_1	1665950	1667410	STR_RS05265	0.0	Capsule	Immune modulation	96.85	100.21	WP_041827043
FGFKEIIK_1	1668151	1668843	STR_RS05255	0.0	Capsule	Immune modulation	95.38	100.00	WP_011227247
FGFKEIIK_1	1668151	1668843	STU_RS14640	0.0	Capsule	Immune modulation	95.38	100.00	WP_011226047
FGFKEIIK_1	1329672	1330739	STU_RS16235	0.0	Capsule	Immune modulation	95.27	101.14	WP_002948022
FGFKEIIK_1	1669650	1671015	STER_RS05300	0.0	Capsule	Immune modulation	94.58	99.85	WP_011681173
FGFKEIIK_1	1668151	1668843	STER_RS05310	0.0	Capsule	Immune modulation	90.48	100.00	WP_011681175
FGFKEIIK_1	1321614	1322888	STU_RS16265	0.0	Capsule	Immune modulation	89.42	99.92	WP_011226331
FGFKEIIK_1	214096	215399	eno	0.0	Streptococcal enolase	Exoenzyme	88.02	99.46	WP_000022832
FGFKEIIK_1	1033113	1034123	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
FGFKEIIK_1	674923	676206	tig/ropA	0.0	Trigger factor	Stress survival	80.81	99.77	WP_000107753
FGFKEIIK_1	1541522	1542568	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
FGFKEIIK_1	1681394	1681977	SPD_RS01775	1.10e-151	Capsule	Immune modulation	83.59	96.20	WP_001819836

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
FGFKEIIK_1	1500859	1499876	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
FGFKEIIK_1	96128	95055	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
FGFKEIIK_1	1157103	1154800	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
FGFKEIIK_1	299037	298069	spr0084	0.0	pathogenitcity related	92.57	98.48	AAK98888
FGFKEIIK_1	67808	65562	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
FGFKEIIK_1	1430547	1429066	SPD_0310	0.0	UPF0371 protein SPD_0310	91.70	100.00	ABJ54480
FGFKEIIK_1	752806	751808	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
FGFKEIIK_1	788920	787949	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
FGFKEIIK_1	1271120	1272271	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
FGFKEIIK_1	1306483	1308471	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
FGFKEIIK_1	1517058	1518788	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
FGFKEIIK_1	827083	828561	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
FGFKEIIK_1	215399	214098	Eno	0.0	enolase	86.21	99.77	ABP90468
FGFKEIIK_1	842272	844164	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
FGFKEIIK_1	62637	64346	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
FGFKEIIK_1	990725	989715	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
FGFKEIIK_1	1762540	1763901	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
FGFKEIIK_1	940381	942273	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
FGFKEIIK_1	229835	228945	bglB	5.30e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
FGFKEIIK_1	1841149	1842087	SP_0676	2.38e-167	transcription facot	81.15	97.20	AAK74821
FGFKEIIK_1	65370	64513	metF	1.54e-164	methionine biosynthesis enzymes	86.36	99.31	AAK74739
FGFKEIIK_1	1627748	1628452	covR	4.42e-131	responder protein	85.53	100.00	AAK71319
FGFKEIIK_1	1449522	1450193	rr01	3.03e-124	response regulator	83.93	99.56	CAB54566
FGFKEIIK_1	1627748	1628452	walR	1.86e-121	DNA-binding response regulator	82.13	100.00	ANC99504
FGFKEIIK_1	523177	522491	CovR	1.02e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
FGFKEIIK_1	58683	58015	CiaR	1.32e-117	two-component response regulator	84.31	99.55	AAK74935
FGFKEIIK_1	58683	58012	ciaR	1.39e-115	two component system response regulator	82.14	99.12	AIK75708
FGFKEIIK_1	751532	751158	SpxA2	1.16e-69	transcriptional regulator	91.20	94.70	ERL21461
FGFKEIIK_1	1751178	1750777	SpxA1	3.79e-66	transcriptional regulator	80.60	100.00	ERL19273
FGFKEIIK_1	674765	674265	RopE (M5005_Spy_1611)	4.96e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
FGFKEIIK_1	1157103	1154806	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
FGFKEIIK_1	954841	955860	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
FGFKEIIK_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found