Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 8 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BMHOHLJD_11 | 100864 | 102033 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| BMHOHLJD_43 | 93561 | 93878 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BMHOHLJD_43 | 93223 | 93561 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| BMHOHLJD_222 | 39590 | 41029 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| BMHOHLJD_168 | 30778 | 31632 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| BMHOHLJD_168 | 12014 | 13216 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| BMHOHLJD_32 | 9572 | 12142 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BMHOHLJD_75 | 50811 | 52187 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.77 | 100.00 | D12567 |
| BMHOHLJD_168 | 30780 | 31632 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| BMHOHLJD_222 | 66383 | 67302 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BMHOHLJD_75 | 50814 | 52187 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| BMHOHLJD_97 | 49256 | 50896 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.62 | 100.00 | WP_003234144.1 |
| BMHOHLJD_168 | 30781 | 31632 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| BMHOHLJD_75 | 48318 | 48836 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BMHOHLJD_109 | 99825 | 100763 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| BMHOHLJD_167 | 310191 | 311372 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BMHOHLJD_167 | 311855 | 312997 | capA | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391469 |
| BMHOHLJD_167 | 311387 | 311836 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| BMHOHLJD_109 | 103650 | 104435 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| BMHOHLJD_233 | 84999 | 86762 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| BMHOHLJD_109 | 92669 | 99805 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.78 | 100.00 | NP_391076 |
| BMHOHLJD_109 | 102428 | 103624 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| BMHOHLJD_211 | 31219 | 31860 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| BMHOHLJD_109 | 100791 | 102410 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BMHOHLJD_152 | 31767 | 32903 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| BMHOHLJD_181 | 11392 | 10523 | pdxS | 1.98e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| BMHOHLJD_178 | 150526 | 151302 | CodY | 2.13e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BMHOHLJD_109 | 365069 | 365638 | ClpP | 3.10e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BMHOHLJD_65 | 38967 | 38575 | spxA1 | 5.78e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BMHOHLJD_65 | 38967 | 38575 | spx | 3.45e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BMHOHLJD_164 | 34796 | 34554 | SpoVG | 7.53e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BMHOHLJD_97 | 4088 | 4285 | CspA | 3.46e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BMHOHLJD_211 | 43712 | 43906 | CspD | 5.39e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| BMHOHLJD_211 | 43712 | 43909 | CspB | 1.12e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| BMHOHLJD_185 | 6381 | 6574 | Col(SD853) | 194 / 194 | 100.00 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BMHOHLJD_11 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_21 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_32 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_43 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_54 | 1.0 | 0.0 | 1.0 |
| BMHOHLJD_65 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_75 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_86 | 1.0 | 0.0 | 1.0 |
| BMHOHLJD_97 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_108 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_109 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_120 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_130 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_141 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_152 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_161 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_162 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_163 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_164 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_165 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_166 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_167 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_168 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_169 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_170 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_171 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_172 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_173 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_174 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_175 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_176 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_177 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_178 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_179 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_180 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_181 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_182 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_183 | 1.0 | 0.0 | 1.0 |
| BMHOHLJD_184 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_185 | 1.0 | 0.0 | 1.0 |
| BMHOHLJD_186 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_187 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_188 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_189 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_191 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_192 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_193 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_194 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_195 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_196 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_198 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_199 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_200 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_201 | 1.0 | 0.0 | 1.0 |
| BMHOHLJD_202 | 1.0 | 0.0 | 1.0 |
| BMHOHLJD_203 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_205 | 1.0 | 0.0 | 1.0 |
| BMHOHLJD_206 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_211 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_222 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_233 | 0.0 | 1.0 | 0.0 |
| BMHOHLJD_244 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BMHOHLJD_43 | 38147 | 64917 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705, VOG0753, VOG0136 |
| BMHOHLJD_175 | 7049 | 8916 | False | Siphoviridae | VOG3390, VOG4705, VOG5008 |
Phigaro v2.4.0 (Database: Jan. 2024 release)