safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
6	0	0	6.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
AEGDPIPK_11	13026	14960	tet(W)	ARO:3000194	tet(W)	96.87	100.78	AJ222769.3
AEGDPIPK_8	10438	11175	ErmB	ARO:3000375	ErmB	98.78	98.79	AF242872.1
AEGDPIPK_10	5500	6144	vatE	ARO:3002844	vatE	100.00	100.00	AF242872.1
AEGDPIPK_11	4590	6524	tet(W)	ARO:3000194	tet(W)	96.87	100.78	AJ222769.3
AEGDPIPK_8	1675	2412	ErmB	ARO:3000375	ErmB	98.78	98.79	AF242872.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
AEGDPIPK_10	5500	6144	vat(E)	Dalfopristin, Pristinamycin IIA, Virginiamycin M	100.00	100.00	AJ488494
AEGDPIPK_8	10438	11175	erm(B)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.86	100.00	X72021
AEGDPIPK_11	13026	14939	tet(W)	Doxycycline, Tetracycline, Minocycline	99.01	99.69	FN396364

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
AEGDPIPK_10	5500	6141	vat(E)	streptogramin A O-acetyltransferase Vat(E)	EXACTX	100.00	100.00	WP_002328813.1
AEGDPIPK_11	13026	14942	tet(W)	tetracycline resistance ribosomal protection protein Tet(W)	BLASTX	99.69	100.00	WP_000691721.1
AEGDPIPK_11	4590	6506	tet(W)	tetracycline resistance ribosomal protection protein Tet(W)	BLASTX	99.69	100.00	WP_000691721.1
AEGDPIPK_8	10441	11175	erm(B)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	BLASTX	99.59	100.00	WP_001038795.1
AEGDPIPK_8	1678	2412	erm(B)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	BLASTX	99.59	100.00	WP_001038795.1
AEGDPIPK_10	9677	10168	vat(E)	streptogramin A O-acetyltransferase Vat(E)	PARTIALX	92.73	77.10	WP_002328813.1
AEGDPIPK_10	1388	1810	vat(E)	streptogramin A O-acetyltransferase Vat(E)	PARTIALX	91.95	69.63	WP_002328813.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
AEGDPIPK_6	40064	41094	repUS67	1031 / 1039	94.96	CP006012

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
AEGDPIPK_1	0.0	1.0	0.0
AEGDPIPK_2	1.0	0.0	1.0
AEGDPIPK_3	1.0	0.0	1.0
AEGDPIPK_4	0.0	1.0	0.0
AEGDPIPK_5	1.0	0.0	1.0
AEGDPIPK_6	1.0	0.0	1.0
AEGDPIPK_7	1.0	0.0	1.0
AEGDPIPK_8	1.0	0.0	1.0
AEGDPIPK_9	1.0	0.0	1.0
AEGDPIPK_10	1.0	0.0	1.0
AEGDPIPK_11	1.0	0.0	1.0
AEGDPIPK_12	0.0	1.0	0.0
AEGDPIPK_13	0.0	1.0	0.0
AEGDPIPK_14	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
AEGDPIPK_14	1439243	1455783	True	Siphoviridae	VOG0221, VOG0221, VOG9409, VOG9502, VOG0641, VOG4589, VOG0204, VOG4553, VOG4573, VOG4556, VOG4544, VOG4571, VOG5088

Phigaro v2.4.0 (Database: Jan. 2024 release)