safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
IDCOHBHL_1	2735473	2736327	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
IDCOHBHL_1	2716764	2717966	blt	ARO:3003006	blt	100.00	100.00	L32599.1
IDCOHBHL_1	2494446	2495615	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
IDCOHBHL_1	1376655	1376972	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
IDCOHBHL_1	1376317	1376655	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
IDCOHBHL_1	916576	919146	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
IDCOHBHL_1	604534	606177	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
IDCOHBHL_1	338954	339547	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
IDCOHBHL_1	288451	289884	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
IDCOHBHL_1	275636	276556	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
IDCOHBHL_1	2735473	2736327	aadK	Streptomycin	100.00	100.00	M26879
IDCOHBHL_1	275636	276556	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
IDCOHBHL_1	4187473	4188849	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	99.06	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
IDCOHBHL_1	2735476	2736327	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
IDCOHBHL_1	275636	276553	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
IDCOHBHL_1	4184955	4185473	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
IDCOHBHL_1	604537	606177	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
IDCOHBHL_1	4187476	4188849	tet(L)	tetracycline efflux MFS transporter Tet(L)	BLASTX	98.69	100.00	WP_003242953.1
IDCOHBHL_1	2051135	2053729	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
IDCOHBHL_1	3697612	3698754	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
IDCOHBHL_1	3699237	3700418	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
IDCOHBHL_1	3698773	3699222	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
IDCOHBHL_1	2004477	2006240	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
IDCOHBHL_1	3291302	3292087	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
IDCOHBHL_1	3287466	3288404	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
IDCOHBHL_1	3290080	3291276	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
IDCOHBHL_1	3288432	3290051	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
IDCOHBHL_1	3280310	3287446	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
IDCOHBHL_1	2295094	2295735	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
IDCOHBHL_1	132880	134016	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
IDCOHBHL_1	19074	19943	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
IDCOHBHL_1	1689919	1690695	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
IDCOHBHL_1	3546025	3546594	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
IDCOHBHL_1	107477	107861	rep34	385 / 633	100.00	M29725

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
IDCOHBHL_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
IDCOHBHL_1	1320370	1346478	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
IDCOHBHL_1	2666836	2697080	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)