Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 0 | 1 | 2.24 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GPBMILCE_86 | 13644 | 15071 | fexA | ARO:3002704 | fexA | 97.47 | 100.00 | AJ549214.1 |
| GPBMILCE_111 | 2132 | 2884 | ErmB | ARO:3000375 | ErmB | 98.78 | 100.81 | AF242872.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GPBMILCE_111 | 2132 | 2884 | erm(B) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.60 | 100.00 | AF080450 |
| GPBMILCE_86 | 13644 | 15071 | fexA | Chloramphenicol, Florfenicol | 99.16 | 100.00 | AJ549214 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GPBMILCE_111 | 2132 | 2881 | erm(B) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | BLASTX | 98.40 | 100.00 | WP_011018369.1 |
| GPBMILCE_86 | 13644 | 15068 | fexA | chloramphenicol/florfenicol efflux MFS transporter FexA | BLASTX | 97.47 | 100.00 | WP_015585966.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GPBMILCE_103 | 42070 | 42264 | CspA | 9.76e-29 | cold shock protein | 84.61 | 98.48 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| GPBMILCE_116 | 2548 | 3586 | repUS67 | 1039 / 1039 | 97.50 | CP006012 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GPBMILCE_2 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_3 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_5 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_6 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_7 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_9 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_10 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_11 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_12 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_13 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_14 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_15 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_16 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_17 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_19 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_20 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_21 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_22 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_33 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_41 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_42 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_43 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_44 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_45 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_46 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_47 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_48 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_49 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_50 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_51 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_52 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_53 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_54 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_55 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_56 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_57 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_58 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_59 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_60 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_61 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_62 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_63 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_64 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_65 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_66 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_67 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_68 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_69 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_70 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_71 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_72 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_73 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_74 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_75 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_76 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_77 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_78 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_79 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_80 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_81 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_82 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_83 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_84 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_85 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_86 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_87 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_88 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_89 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_90 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_91 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_92 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_93 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_94 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_95 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_96 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_97 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_98 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_99 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_100 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_101 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_102 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_103 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_104 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_105 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_106 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_107 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_108 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_109 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_110 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_111 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_112 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_113 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_114 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_115 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_116 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_117 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_118 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_119 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_120 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_121 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_122 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_123 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_124 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_125 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_126 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_127 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_128 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_129 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_130 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_131 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_132 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_133 | 0.0 | 1.0 | 0.0 |
| GPBMILCE_134 | 1.0 | 0.0 | 1.0 |
| GPBMILCE_136 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GPBMILCE_33 | 20718 | 41653 | False | Siphoviridae | VOG4887, VOG3462, VOG0801, VOG6163, VOG2225, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544 |
| GPBMILCE_45 | 3405 | 32326 | False | Siphoviridae | VOG0753, VOG4918, VOG9998, VOG8870, VOG10972, VOG10957, VOG4599, VOG4605, VOG6163, VOG0660, VOG0209, VOG4586, VOG0207, VOG9844, VOG0205, VOG0204, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG4841, VOG0198, VOG0586, VOG9640, VOG0384, VOG4693, VOG4643, VOG0189, VOG4566, VOG0514 |
| GPBMILCE_48 | 41155 | 85641 | False | Siphoviridae | VOG5361, VOG5229, VOG0703, VOG1637, VOG4626, VOG9997, VOG0565, VOG10972, VOG3462, VOG0801, VOG0083, VOG0209, VOG4586, VOG0207, VOG0205, VOG0204, VOG4553, VOG4573, VOG4556, VOG1886, VOG4581, VOG4841, VOG0198, VOG0650, VOG4585, VOG3307, VOG0195, VOG0275, VOG0536, VOG0189, VOG4799, VOG0226, VOG0283, VOG9708, VOG3478, VOG0707, VOG9469, VOG7881, VOG4586, VOG5063, VOG8520, VOG0862, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)