Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 3 | 5.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EPKFIKFJ_1 | 534148 | 535194 | clbA | ARO:3002814 | clbA | 92.77 | 99.71 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EPKFIKFJ_1 | 534148 | 535191 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.90 | 99.33 | KR610408 |
| EPKFIKFJ_1 | 2590259 | 2591635 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 87.07 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EPKFIKFJ_1 | 534148 | 535191 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 92.24 | 99.71 | WP_012116915.1 |
| EPKFIKFJ_1 | 2590265 | 2591635 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
| EPKFIKFJ_1 | 531879 | 532397 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 82.66 | 100.00 | WP_003242546.1 |
| EPKFIKFJ_1 | 1932169 | 1934766 | rphC | rifamycin-inactivating phosphotransferase RphC | INTERNAL_STOP | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EPKFIKFJ_1 | 3561490 | 3562671 | capB | 0.0 | Capsule | Immune modulation | 81.87 | 99.66 | NP_391471 |
| EPKFIKFJ_1 | 3561026 | 3561475 | capC | 1.51e-113 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EPKFIKFJ_1 | 133405 | 134541 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| EPKFIKFJ_1 | 18627 | 19496 | pdxS | 1.60e-150 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| EPKFIKFJ_1 | 1691856 | 1692632 | CodY | 3.37e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EPKFIKFJ_1 | 0.0 | 1.0 | 0.0 |
| EPKFIKFJ_2 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EPKFIKFJ_1 | 600944 | 614901 | False | Siphoviridae | VOG4602, VOG8483, VOG9667, VOG11075, VOG0650, VOG4552, VOG4600, VOG1309, VOG9093, VOG0189, VOG0044, VOG0536, VOG1336, VOG4658, VOG8569, VOG0397, VOG6581 |
| EPKFIKFJ_1 | 617273 | 646340 | False | Siphoviridae | VOG5447, VOG4972, VOG4681, VOG0796, VOG10227, VOG0720, VOG4564, VOG6778, VOG4555, VOG1329, VOG2621, VOG5872, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG4545, VOG4605, VOG4599, VOG3390, VOG5535, VOG0753, VOG0136, VOG4574 |
| EPKFIKFJ_1 | 916037 | 948780 | False | Siphoviridae | VOG6495, VOG0181, VOG4566, VOG0189, VOG6931, VOG6587, VOG1336, VOG5407, VOG4658, VOG8569, VOG0397, VOG6581, VOG6517, VOG6242, VOG0198, VOG0275, VOG7884, VOG0531, VOG0519, VOG4544, VOG4556, VOG4573, VOG4553, VOG3435, VOG0204, VOG4589, VOG0704, VOG0539, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641 |
| EPKFIKFJ_1 | 1260280 | 1266784 | True | Siphoviridae | VOG6282, VOG3699, VOG0221, VOG7734, VOG0720, VOG4555, VOG4572 |
| EPKFIKFJ_1 | 1341365 | 1368169 | False | Myoviridae | VOG9667, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
| EPKFIKFJ_1 | 1878204 | 1915698 | False | Siphoviridae | VOG0275, VOG4602, VOG9667, VOG6495, VOG4552, VOG0650, VOG7438, VOG1047, VOG0021, VOG1565, VOG0026, VOG4567, VOG4609, VOG0327, VOG4548, VOG0198, VOG4581, VOG4544, VOG5051, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641 |
| EPKFIKFJ_1 | 2624960 | 2653255 | False | Siphoviridae | VOG2490, VOG4574, VOG0641, VOG7896, VOG3390, VOG4612, VOG4660, VOG4772, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG5051, VOG4544, VOG4581, VOG7884, VOG0275, VOG0198, VOG6242, VOG6517, VOG6581, VOG0397, VOG1669, VOG4658 |
| EPKFIKFJ_1 | 3010487 | 3037591 | False | Siphoviridae | VOG5216, VOG10051, VOG6116, VOG7508, VOG0602, VOG4824, VOG5221, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5601, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841 |
| EPKFIKFJ_1 | 3044928 | 3049153 | False | Siphoviridae | VOG8420, VOG4799, VOG9755, VOG0052, VOG10904 |
Phigaro v2.4.0 (Database: Jan. 2024 release)