Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 9 | 9 | 16.82 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IFGMGPOA_12 | 423322 | 423660 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| IFGMGPOA_12 | 423005 | 423322 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| IFGMGPOA_17 | 351400 | 352569 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| IFGMGPOA_33 | 252396 | 254966 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| IFGMGPOA_36 | 109567 | 110487 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| IFGMGPOA_36 | 96235 | 97674 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| IFGMGPOA_36 | 46586 | 47179 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| IFGMGPOA_43 | 33377 | 34240 | aadK | ARO:3002627 | aadK | 97.89 | 101.06 | AL009126.1 |
| IFGMGPOA_34 | 28879 | 30525 | vmlR | ARO:3004476 | vmlR | 99.45 | 100.18 | NC_000964.3 |
| IFGMGPOA_43 | 14800 | 16002 | blt | ARO:3003006 | blt | 98.75 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IFGMGPOA_36 | 109567 | 110487 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| IFGMGPOA_43 | 33388 | 34240 | aadK | Streptomycin | 98.12 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IFGMGPOA_34 | 28879 | 30522 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.45 | 100.00 | WP_003234144.1 |
| IFGMGPOA_36 | 109570 | 110487 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| IFGMGPOA_43 | 33389 | 34240 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| IFGMGPOA_17 | 60891 | 63488 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IFGMGPOA_5 | 156910 | 158091 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IFGMGPOA_17 | 154082 | 154723 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| IFGMGPOA_7 | 103254 | 104039 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| IFGMGPOA_7 | 104065 | 105261 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| IFGMGPOA_17 | 6497 | 8260 | dep/capD | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_389723 |
| IFGMGPOA_5 | 158574 | 159716 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| IFGMGPOA_7 | 106937 | 107875 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391077 |
| IFGMGPOA_5 | 158106 | 158555 | capC | 0.0 | Capsule | Immune modulation | 98.00 | 100.00 | NP_391470 |
| IFGMGPOA_7 | 105290 | 106909 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.35 | 100.00 | NP_391078 |
| IFGMGPOA_8 | 1 | 4731 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.29 | 66.29 | NP_391076 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IFGMGPOA_42 | 31672 | 32808 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IFGMGPOA_45 | 4265 | 5134 | pdxS | 1.10e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IFGMGPOA_12 | 111781 | 111005 | CodY | 3.77e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IFGMGPOA_5 | 312325 | 311756 | ClpP | 2.38e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| IFGMGPOA_14 | 92413 | 92021 | spxA1 | 1.29e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| IFGMGPOA_14 | 92413 | 92021 | spx | 7.72e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| IFGMGPOA_44 | 20513 | 20755 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| IFGMGPOA_34 | 77628 | 77431 | CspA | 4.61e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| IFGMGPOA_17 | 165954 | 166148 | CspD | 1.31e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| IFGMGPOA_15 | 134070 | 134264 | CspB | 1.51e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IFGMGPOA_1 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_3 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_4 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_5 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_6 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_7 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_8 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_9 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_10 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_11 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_12 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_13 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_14 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_15 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_16 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_17 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_18 | 1.0 | 0.0 | 1.0 |
| IFGMGPOA_20 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_21 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_23 | 1.0 | 0.0 | 1.0 |
| IFGMGPOA_25 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_26 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_27 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_28 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_29 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_30 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_32 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_33 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_34 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_35 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_36 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_37 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_38 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_39 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_40 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_41 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_42 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_43 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_44 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_45 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_46 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_47 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_48 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_49 | 0.0 | 1.0 | 0.0 |
| IFGMGPOA_50 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IFGMGPOA_12 | 453533 | 460603 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894 |
| IFGMGPOA_20 | 8017 | 29771 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG5668, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG9228, VOG4688 |
Phigaro v2.4.0 (Database: Jan. 2024 release)