Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 1 | 4 | 5.74 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MFPGIFIB_39 | 90322 | 91374 | clbC | ARO:3002816 | clbC | 97.14 | 100.00 | AP006627.1 |
| MFPGIFIB_52 | 33018 | 33863 | Erm(34) | ARO:3000600 | Erm(34) | 98.58 | 100.00 | AY234334.1 |
| MFPGIFIB_85 | 6637 | 7407 | ANT(4')-Ib | ARO:3003905 | ANT(4')-Ib | 94.14 | 100.00 | EF540343.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MFPGIFIB_52 | 33018 | 33863 | erm(34) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 97.87 | 100.00 | AY234334 |
| MFPGIFIB_85 | 6637 | 7407 | ant(4')-Ib | Amikacin, Tobramycin, Isepamicin, Dibekacin | 94.16 | 100.00 | AJ506108 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MFPGIFIB_52 | 33018 | 33860 | erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | BLASTX | 98.58 | 100.00 | WP_063844504.1 |
| MFPGIFIB_39 | 90325 | 91374 | clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | BLASTX | 97.14 | 100.00 | WP_011245929.1 |
| MFPGIFIB_85 | 3321 | 4223 | blaBCL | BCL family class A beta-lactamase | BLASTX | 96.68 | 100.00 | WP_065825670.1 |
| MFPGIFIB_85 | 6640 | 7407 | aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | BLASTX | 94.14 | 100.00 | WP_035202393.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MFPGIFIB_5 | 17846 | 18496 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 96.77 | 100.00 | WP_011247375 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MFPGIFIB_82 | 7368 | 8510 | tufA | 0.0 | elongation factor Tu | 87.14 | 96.19 | AHM69299 |
| MFPGIFIB_103 | 16213 | 15971 | SpoVG | 1.17e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MFPGIFIB_106 | 1605 | 1802 | CspA | 5.82e-31 | cold shock protein | 86.36 | 100.00 | CAA62903 |
| MFPGIFIB_99 | 20261 | 20073 | CspB | 5.47e-26 | cold shock protein | 82.54 | 95.45 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MFPGIFIB_1 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_2 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_3 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_4 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_5 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_6 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_7 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_8 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_9 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_10 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_11 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_12 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_13 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_14 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_15 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_16 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_20 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_21 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_22 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_23 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_24 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_25 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_26 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_27 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_28 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_29 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_30 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_31 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_32 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_33 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_34 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_35 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_36 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_37 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_38 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_39 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_40 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_41 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_42 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_43 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_44 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_46 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_48 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_49 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_51 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_52 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_53 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_54 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_55 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_56 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_57 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_58 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_59 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_60 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_61 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_62 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_63 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_64 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_66 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_67 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_68 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_69 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_70 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_71 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_72 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_73 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_74 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_75 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_76 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_77 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_78 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_79 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_80 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_81 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_82 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_83 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_84 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_85 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_86 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_87 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_88 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_90 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_91 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_92 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_93 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_94 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_95 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_96 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_97 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_98 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_99 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_100 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_101 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_102 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_103 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_104 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_105 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_106 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_107 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_108 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_109 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_110 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_111 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_112 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_113 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_114 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_115 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_116 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_117 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_118 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_119 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_120 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_121 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_122 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_123 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_124 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_125 | 0.0 | 1.0 | 0.0 |
| MFPGIFIB_127 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_129 | 1.0 | 0.0 | 1.0 |
| MFPGIFIB_130 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MFPGIFIB_23 | 16626 | 43100 | False | Siphoviridae | VOG4552, VOG6242, VOG5447, VOG6780, VOG5361, VOG0796, VOG4544, VOG0720, VOG4564, VOG4555, VOG3434, VOG1887, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG6163, VOG0801, VOG4619, VOG0565, VOG4669, VOG0753, VOG0602 |
Phigaro v2.4.0 (Database: Jan. 2024 release)