Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 1 | 4 | 6.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EDDHANJI_2 | 249137 | 249784 | Alkalihalobacillus clausii chloramphenicol acetyltransferase | ARO:3004452 | Acla_ACT_CHL | 88.21 | 94.30 | AY238971.1 |
| EDDHANJI_5 | 178433 | 179278 | Erm(34) | ARO:3000600 | Erm(34) | 96.80 | 100.00 | AY234334.1 |
| EDDHANJI_33 | 68209 | 68979 | ANT(4')-Ib | ARO:3003905 | ANT(4')-Ib | 98.05 | 100.00 | EF540343.1 |
| EDDHANJI_57 | 26033 | 26938 | BCL-1 | ARO:3004749 | BCL-1 | 99.67 | 98.05 | EF540343.1 |
| EDDHANJI_20 | 2885 | 3937 | clbC | ARO:3002816 | clbC | 99.43 | 100.00 | AP006627.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EDDHANJI_33 | 68209 | 68979 | ant(4')-Ib | Amikacin, Tobramycin, Isepamicin, Dibekacin | 98.19 | 99.74 | AJ506108 |
| EDDHANJI_2 | 249106 | 249782 | cat | Chloramphenicol | 97.23 | 98.40 | AY238971 |
| EDDHANJI_5 | 178433 | 179265 | erm(34) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 96.04 | 98.46 | AY234334 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EDDHANJI_57 | 26036 | 26938 | blaBCL | BCL family class A beta-lactamase | BLASTX | 99.67 | 100.00 | WP_065825670.1 |
| EDDHANJI_2 | 249140 | 249784 | catA | type A-10 chloramphenicol O-acetyltransferase | BLASTX | 99.53 | 100.00 | WP_035203840.1 |
| EDDHANJI_20 | 2888 | 3937 | clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | BLASTX | 99.43 | 100.00 | WP_011245929.1 |
| EDDHANJI_33 | 68209 | 68976 | aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | BLASTX | 98.05 | 100.00 | WP_035202393.1 |
| EDDHANJI_5 | 178433 | 179272 | erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | BLASTX | 97.14 | 99.64 | WP_063844504.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EDDHANJI_11 | 22874 | 23524 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | WP_011247375 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EDDHANJI_36 | 32611 | 33747 | tufA | 0.0 | elongation factor Tu | 87.33 | 95.69 | AHM69299 |
| EDDHANJI_41 | 21879 | 21637 | SpoVG | 1.41e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| EDDHANJI_58 | 23868 | 23674 | CspA | 1.32e-30 | cold shock protein | 86.15 | 98.48 | CAA62903 |
| EDDHANJI_40 | 12206 | 12397 | msaB | 1.24e-26 | cold shock protein | 81.25 | 96.97 | ABD22065 |
| EDDHANJI_40 | 12209 | 12397 | CspB | 1.07e-25 | cold shock protein | 80.95 | 95.45 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EDDHANJI_1 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_2 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_3 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_4 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_5 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_6 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_7 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_8 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_9 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_10 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_11 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_12 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_13 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_14 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_15 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_16 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_17 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_18 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_19 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_20 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_21 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_22 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_23 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_24 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_25 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_26 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_27 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_28 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_30 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_31 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_32 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_33 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_34 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_35 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_36 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_37 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_38 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_39 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_40 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_41 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_42 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_43 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_44 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_45 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_46 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_47 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_49 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_50 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_51 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_52 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_53 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_54 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_55 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_56 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_57 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_58 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_59 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_60 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_61 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_62 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_63 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_64 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_65 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_66 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_67 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_68 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_69 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_70 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_71 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_72 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_73 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_74 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_75 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_76 | 1.0 | 0.0 | 1.0 |
| EDDHANJI_77 | 0.0 | 1.0 | 0.0 |
| EDDHANJI_78 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EDDHANJI_15 | 2860 | 35841 | False | Siphoviridae | VOG4581, VOG4544, VOG3607, VOG4556, VOG4568, VOG4553, VOG7168, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG4910, VOG0801, VOG4660, VOG5384, VOG3390, VOG4705, VOG4619, VOG0641, VOG8236, VOG0018, VOG6116, VOG0866, VOG4602, VOG8520, VOG5353, VOG0468, VOG6414, VOG6931, VOG0535, VOG1936 |
Phigaro v2.4.0 (Database: Jan. 2024 release)