Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FGGCBLHG_25 | 504197 | 504535 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| FGGCBLHG_25 | 503880 | 504197 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| FGGCBLHG_4 | 258221 | 259390 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| FGGCBLHG_30 | 187509 | 189155 | vmlR | ARO:3004476 | vmlR | 99.09 | 100.18 | NC_000964.3 |
| FGGCBLHG_32 | 86110 | 87549 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| FGGCBLHG_32 | 73297 | 74217 | mphK | ARO:3004541 | mphK | 97.06 | 100.00 | CP010314.1 |
| FGGCBLHG_29 | 71099 | 73669 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| FGGCBLHG_9 | 31886 | 32740 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| FGGCBLHG_33 | 20026 | 20619 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| FGGCBLHG_9 | 14746 | 15948 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FGGCBLHG_9 | 31886 | 32740 | aadK | Streptomycin | 98.48 | 100.00 | M26879 |
| FGGCBLHG_32 | 73297 | 74217 | mph(K) | Spiramycin, Telithromycin | 97.18 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FGGCBLHG_30 | 187512 | 189155 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.09 | 100.00 | WP_003234144.1 |
| FGGCBLHG_9 | 31889 | 32740 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| FGGCBLHG_32 | 73297 | 74214 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.06 | 100.00 | WP_003246254.1 |
| FGGCBLHG_13 | 37644 | 38162 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| FGGCBLHG_3 | 57362 | 59959 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.83 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FGGCBLHG_18 | 14335 | 15516 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| FGGCBLHG_21 | 298707 | 299645 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| FGGCBLHG_4 | 60925 | 61566 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| FGGCBLHG_21 | 295024 | 295809 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| FGGCBLHG_21 | 299665 | 306801 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.41 | 100.00 | NP_391076 |
| FGGCBLHG_18 | 15531 | 15980 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| FGGCBLHG_18 | 15999 | 17141 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| FGGCBLHG_3 | 6636 | 8399 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| FGGCBLHG_21 | 295835 | 297031 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| FGGCBLHG_21 | 297060 | 298679 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.53 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FGGCBLHG_34 | 27923 | 26787 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| FGGCBLHG_36 | 11179 | 10310 | pdxS | 1.10e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| FGGCBLHG_25 | 192560 | 191784 | CodY | 4.77e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FGGCBLHG_21 | 35899 | 35330 | ClpP | 1.99e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| FGGCBLHG_26 | 66149 | 65757 | spxA1 | 6.76e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| FGGCBLHG_26 | 66149 | 65757 | spx | 4.03e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| FGGCBLHG_35 | 20513 | 20755 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FGGCBLHG_30 | 141076 | 141273 | CspA | 9.42e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| FGGCBLHG_4 | 72799 | 72993 | CspD | 1.07e-27 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| FGGCBLHG_27 | 204645 | 204839 | CspB | 2.16e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FGGCBLHG_2 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_3 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_4 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_5 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_6 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_7 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_9 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_10 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_11 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_12 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_13 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_14 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_15 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_16 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_17 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_18 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_19 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_20 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_21 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_22 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_23 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_25 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_26 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_27 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_28 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_29 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_30 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_31 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_32 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_33 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_34 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_35 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_36 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_38 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_39 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_40 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_41 | 0.0 | 1.0 | 0.0 |
| FGGCBLHG_42 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FGGCBLHG_7 | 3157 | 26680 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG7060 |
| FGGCBLHG_10 | 2066 | 25335 | False | Myoviridae | VOG5859, VOG4574, VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555, VOG0720 |
| FGGCBLHG_25 | 534367 | 560408 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)