Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 1 | 4 | 6.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BICANOEC_12 | 95430 | 96275 | Erm(34) | ARO:3000600 | Erm(34) | 96.80 | 100.00 | AY234334.1 |
| BICANOEC_3 | 81676 | 82323 | Alkalihalobacillus clausii chloramphenicol acetyltransferase | ARO:3004452 | Acla_ACT_CHL | 88.21 | 94.30 | AY238971.1 |
| BICANOEC_52 | 32791 | 33561 | ANT(4')-Ib | ARO:3003905 | ANT(4')-Ib | 98.05 | 100.00 | EF540343.1 |
| BICANOEC_52 | 29472 | 30377 | BCL-1 | ARO:3004749 | BCL-1 | 99.67 | 98.05 | EF540343.1 |
| BICANOEC_42 | 6165 | 7217 | clbC | ARO:3002816 | clbC | 99.43 | 100.00 | AP006627.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BICANOEC_52 | 32791 | 33561 | ant(4')-Ib | Amikacin, Tobramycin, Isepamicin, Dibekacin | 98.96 | 100.00 | AJ506108 |
| BICANOEC_3 | 81678 | 82354 | cat | Chloramphenicol | 97.37 | 98.40 | AY238971 |
| BICANOEC_12 | 95430 | 96262 | erm(34) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 96.04 | 98.46 | AY234334 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BICANOEC_52 | 29475 | 30377 | blaBCL | BCL family class A beta-lactamase | BLASTX | 99.67 | 100.00 | WP_065825670.1 |
| BICANOEC_3 | 81676 | 82320 | catA | type A-10 chloramphenicol O-acetyltransferase | BLASTX | 99.53 | 100.00 | WP_035203840.1 |
| BICANOEC_42 | 6165 | 7214 | clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | BLASTX | 99.43 | 100.00 | WP_011245929.1 |
| BICANOEC_52 | 32794 | 33561 | aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | BLASTX | 98.05 | 100.00 | WP_035202393.1 |
| BICANOEC_12 | 95430 | 96269 | erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | BLASTX | 97.14 | 99.64 | WP_063844504.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BICANOEC_21 | 124325 | 124975 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.85 | 100.00 | WP_011247375 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BICANOEC_57 | 7334 | 8470 | tufA | 0.0 | elongation factor Tu | 87.33 | 95.69 | AHM69299 |
| BICANOEC_55 | 42465 | 42223 | SpoVG | 2.55e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BICANOEC_22 | 29408 | 29214 | CspA | 1.75e-30 | cold shock protein | 86.15 | 98.48 | CAA62903 |
| BICANOEC_60 | 25291 | 25482 | msaB | 1.06e-26 | cold shock protein | 81.25 | 96.97 | ABD22065 |
| BICANOEC_60 | 25294 | 25482 | CspB | 9.12e-26 | cold shock protein | 80.95 | 95.45 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BICANOEC_1 | 0.0 | 1.0 | 0.0 |
| BICANOEC_2 | 0.0 | 1.0 | 0.0 |
| BICANOEC_3 | 0.0 | 1.0 | 0.0 |
| BICANOEC_4 | 0.0 | 1.0 | 0.0 |
| BICANOEC_5 | 1.0 | 0.0 | 1.0 |
| BICANOEC_6 | 0.0 | 1.0 | 0.0 |
| BICANOEC_7 | 0.0 | 1.0 | 0.0 |
| BICANOEC_8 | 0.0 | 1.0 | 0.0 |
| BICANOEC_9 | 0.0 | 1.0 | 0.0 |
| BICANOEC_10 | 0.0 | 1.0 | 0.0 |
| BICANOEC_12 | 0.0 | 1.0 | 0.0 |
| BICANOEC_13 | 0.0 | 1.0 | 0.0 |
| BICANOEC_14 | 0.0 | 1.0 | 0.0 |
| BICANOEC_15 | 1.0 | 0.0 | 1.0 |
| BICANOEC_16 | 1.0 | 0.0 | 1.0 |
| BICANOEC_17 | 0.0 | 1.0 | 0.0 |
| BICANOEC_18 | 0.0 | 1.0 | 0.0 |
| BICANOEC_19 | 0.0 | 1.0 | 0.0 |
| BICANOEC_20 | 0.0 | 1.0 | 0.0 |
| BICANOEC_21 | 0.0 | 1.0 | 0.0 |
| BICANOEC_22 | 0.0 | 1.0 | 0.0 |
| BICANOEC_23 | 0.0 | 1.0 | 0.0 |
| BICANOEC_24 | 0.0 | 1.0 | 0.0 |
| BICANOEC_25 | 0.0 | 1.0 | 0.0 |
| BICANOEC_26 | 0.0 | 1.0 | 0.0 |
| BICANOEC_27 | 0.0 | 1.0 | 0.0 |
| BICANOEC_28 | 1.0 | 0.0 | 1.0 |
| BICANOEC_29 | 0.0 | 1.0 | 0.0 |
| BICANOEC_30 | 0.0 | 1.0 | 0.0 |
| BICANOEC_32 | 0.0 | 1.0 | 0.0 |
| BICANOEC_33 | 0.0 | 1.0 | 0.0 |
| BICANOEC_34 | 0.0 | 1.0 | 0.0 |
| BICANOEC_35 | 1.0 | 0.0 | 1.0 |
| BICANOEC_36 | 0.0 | 1.0 | 0.0 |
| BICANOEC_37 | 0.0 | 1.0 | 0.0 |
| BICANOEC_38 | 0.0 | 1.0 | 0.0 |
| BICANOEC_39 | 0.0 | 1.0 | 0.0 |
| BICANOEC_40 | 1.0 | 0.0 | 1.0 |
| BICANOEC_41 | 1.0 | 0.0 | 1.0 |
| BICANOEC_42 | 0.0 | 1.0 | 0.0 |
| BICANOEC_43 | 0.0 | 1.0 | 0.0 |
| BICANOEC_45 | 0.0 | 1.0 | 0.0 |
| BICANOEC_46 | 0.0 | 1.0 | 0.0 |
| BICANOEC_47 | 0.0 | 1.0 | 0.0 |
| BICANOEC_48 | 0.0 | 1.0 | 0.0 |
| BICANOEC_49 | 1.0 | 0.0 | 1.0 |
| BICANOEC_50 | 1.0 | 0.0 | 1.0 |
| BICANOEC_51 | 0.0 | 1.0 | 0.0 |
| BICANOEC_52 | 0.0 | 1.0 | 0.0 |
| BICANOEC_53 | 0.0 | 1.0 | 0.0 |
| BICANOEC_54 | 0.0 | 1.0 | 0.0 |
| BICANOEC_55 | 0.0 | 1.0 | 0.0 |
| BICANOEC_56 | 0.0 | 1.0 | 0.0 |
| BICANOEC_57 | 0.0 | 1.0 | 0.0 |
| BICANOEC_58 | 0.0 | 1.0 | 0.0 |
| BICANOEC_59 | 0.0 | 1.0 | 0.0 |
| BICANOEC_60 | 0.0 | 1.0 | 0.0 |
| BICANOEC_61 | 0.0 | 1.0 | 0.0 |
| BICANOEC_62 | 0.0 | 1.0 | 0.0 |
| BICANOEC_63 | 1.0 | 0.0 | 1.0 |
| BICANOEC_64 | 1.0 | 0.0 | 1.0 |
| BICANOEC_65 | 0.0 | 1.0 | 0.0 |
| BICANOEC_66 | 0.0 | 1.0 | 0.0 |
| BICANOEC_67 | 1.0 | 0.0 | 1.0 |
| BICANOEC_68 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BICANOEC_2 | 17267 | 35928 | False | Siphoviridae | VOG6242, VOG5447, VOG5361, VOG0796, VOG4544, VOG0720, VOG4564, VOG4555, VOG3434, VOG1887, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG6163, VOG0727 |
Phigaro v2.4.0 (Database: Jan. 2024 release)