Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 1 | 4 | 6.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FGLPCCAC_4 | 347271 | 347918 | Alkalihalobacillus clausii chloramphenicol acetyltransferase | ARO:3004452 | Acla_ACT_CHL | 87.74 | 94.30 | AY238971.1 |
| FGLPCCAC_10 | 60242 | 61087 | Erm(34) | ARO:3000600 | Erm(34) | 96.80 | 100.00 | AY234334.1 |
| FGLPCCAC_36 | 26714 | 27484 | ANT(4')-Ib | ARO:3003905 | ANT(4')-Ib | 98.05 | 100.00 | EF540343.1 |
| FGLPCCAC_36 | 23395 | 24319 | BCL-1 | ARO:3004749 | BCL-1 | 100.00 | 98.05 | EF540343.1 |
| FGLPCCAC_42 | 9187 | 10239 | clbC | ARO:3002816 | clbC | 99.14 | 100.00 | AP006627.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FGLPCCAC_36 | 26714 | 27484 | ant(4')-Ib | Amikacin, Tobramycin, Isepamicin, Dibekacin | 98.18 | 100.00 | AJ506108 |
| FGLPCCAC_4 | 347240 | 347916 | cat | Chloramphenicol | 96.93 | 98.40 | AY238971 |
| FGLPCCAC_10 | 60255 | 61087 | erm(34) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 95.92 | 98.46 | AY234334 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FGLPCCAC_36 | 23398 | 24300 | blaBCL-1 | class A beta-lactamase BCL-1 | ALLELEX | 100.00 | 100.00 | WP_065825670.1 |
| FGLPCCAC_42 | 9187 | 10236 | clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | BLASTX | 99.14 | 100.00 | WP_011245929.1 |
| FGLPCCAC_4 | 347274 | 347918 | catA | type A-10 chloramphenicol O-acetyltransferase | BLASTX | 99.07 | 100.00 | WP_035203840.1 |
| FGLPCCAC_36 | 26717 | 27484 | aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | BLASTX | 98.05 | 100.00 | WP_035202393.1 |
| FGLPCCAC_10 | 60248 | 61087 | erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | BLASTX | 97.14 | 99.64 | WP_063844504.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FGLPCCAC_8 | 123425 | 124075 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.85 | 100.00 | WP_011247375 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FGLPCCAC_45 | 32555 | 33691 | tufA | 0.0 | elongation factor Tu | 87.33 | 95.69 | AHM69299 |
| FGLPCCAC_44 | 42435 | 42193 | SpoVG | 2.55e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FGLPCCAC_19 | 6487 | 6681 | CspA | 1.62e-30 | cold shock protein | 86.15 | 98.48 | CAA62903 |
| FGLPCCAC_6 | 25088 | 25279 | msaB | 7.64e-26 | cold shock protein | 81.25 | 96.97 | ABD22065 |
| FGLPCCAC_6 | 25091 | 25279 | CspB | 6.56e-25 | cold shock protein | 80.95 | 95.45 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FGLPCCAC_1 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_2 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_3 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_4 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_5 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_6 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_7 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_8 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_9 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_10 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_11 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_12 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_13 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_14 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_15 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_16 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_17 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_19 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_23 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_24 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_25 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_26 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_27 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_28 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_29 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_30 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_31 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_32 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_33 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_34 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_35 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_36 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_37 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_38 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_39 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_40 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_42 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_43 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_44 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_45 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_47 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_48 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_50 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_52 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_55 | 0.0 | 1.0 | 0.0 |
| FGLPCCAC_56 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_58 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_59 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_60 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_61 | 1.0 | 0.0 | 1.0 |
| FGLPCCAC_62 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FGLPCCAC_2 | 3972 | 10711 | False | Siphoviridae | VOG10904, VOG4561, VOG1563, VOG11077, VOG4552, VOG2664, VOG9600, VOG0025, VOG1884 |
| FGLPCCAC_2 | 16515 | 31234 | False | Siphoviridae | VOG0796, VOG10227, VOG0720, VOG4564, VOG4555, VOG3434, VOG1887, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG5361, VOG0725, VOG0800 |
Phigaro v2.4.0 (Database: Jan. 2024 release)