safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	23	27	35.47

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
MDDNCMGL_1	1147982	1148851	rfbA	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681286
MDDNCMGL_1	1147389	1147982	STER_RS06030	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
MDDNCMGL_1	1371851	1372828	STER_RS07095	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681444
MDDNCMGL_1	1372825	1374084	STER_RS07100	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681445
MDDNCMGL_1	1147389	1147982	STR_RS05880	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
MDDNCMGL_1	1147389	1147982	STU_RS15260	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
MDDNCMGL_1	904486	906144	fbp54	0.0	FBPs	Adherence	99.64	100.00	WP_011227179
MDDNCMGL_1	1369032	1369802	STER_RS07075	0.0	Capsule	Immune modulation	99.61	99.23	WP_011681440
MDDNCMGL_1	43729	44568	cbpD	0.0	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
MDDNCMGL_1	1370865	1371833	STER_RS07090	0.0	Capsule	Immune modulation	99.48	100.00	WP_011681443
MDDNCMGL_1	1370148	1370863	STER_RS07085	0.0	Capsule	Immune modulation	99.30	99.86	WP_011681442
MDDNCMGL_1	1022055	1022786	STER_RS05315	0.0	Capsule	Immune modulation	98.91	100.00	WP_011681176
MDDNCMGL_1	1022055	1022786	STU_RS14645	0.0	Capsule	Immune modulation	98.63	100.00	WP_011226048
MDDNCMGL_1	1182753	1183514	srtA	0.0	Sortase A	Exoenzyme	98.56	100.00	-
MDDNCMGL_1	1022055	1022786	STR_RS05260	0.0	Capsule	Immune modulation	98.50	100.00	WP_011227248
MDDNCMGL_1	1010628	1012043	STER_RS05250	0.0	Capsule	Immune modulation	98.38	100.00	WP_011681165
MDDNCMGL_1	1089835	1090628	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	98.36	100.25	-
MDDNCMGL_1	1020604	1021344	STU_RS14635	0.0	Capsule	Immune modulation	98.25	100.00	WP_011226046
MDDNCMGL_1	1021354	1022046	STR_RS05255	0.0	Capsule	Immune modulation	97.98	100.00	WP_011227247
MDDNCMGL_1	1021354	1022046	STU_RS14640	0.0	Capsule	Immune modulation	97.98	100.00	WP_011226047
MDDNCMGL_1	1022787	1024247	STER_RS05320	0.0	Capsule	Immune modulation	97.88	100.00	WP_011681177
MDDNCMGL_1	1020604	1021344	STER_RS05305	0.0	Capsule	Immune modulation	97.84	100.00	WP_011681174
MDDNCMGL_1	1020609	1021344	STR_RS05250	0.0	Capsule	Immune modulation	97.28	98.13	WP_011227246
MDDNCMGL_1	1019180	1020547	STU_RS14630	0.0	Capsule	Immune modulation	97.00	100.00	WP_011226045
MDDNCMGL_1	1022787	1024247	STU_RS14650	0.0	Capsule	Immune modulation	96.99	100.21	WP_011226049
MDDNCMGL_1	1374203	1375054	rfbD	0.0	Capsule	Immune modulation	96.83	100.00	WP_014621820
MDDNCMGL_1	1022787	1024247	STR_RS05265	0.0	Capsule	Immune modulation	96.03	100.21	WP_041827043
MDDNCMGL_1	1019182	1020547	STER_RS05300	0.0	Capsule	Immune modulation	94.58	99.85	WP_011681173
MDDNCMGL_1	1021354	1022046	STER_RS05310	0.0	Capsule	Immune modulation	90.19	100.00	WP_011681175
MDDNCMGL_1	622237	623540	eno	0.0	Streptococcal enolase	Exoenzyme	88.17	99.54	WP_000022832
MDDNCMGL_1	1651037	1652047	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.66	99.90	WP_002986042
MDDNCMGL_1	158612	159895	tig/ropA	0.0	Trigger factor	Stress survival	80.73	99.77	WP_000107753
MDDNCMGL_1	1145914	1146960	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
MDDNCMGL_1	1008260	1008846	SPD_RS01775	1.11e-141	Capsule	Immune modulation	82.48	96.70	WP_001819836

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
MDDNCMGL_1	1186201	1187184	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
MDDNCMGL_1	732212	733285	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
MDDNCMGL_1	1541001	1543304	pfl2(Sp_0459)	0.0	formate acetyltransferase	93.10	99.22	AAK74619
MDDNCMGL_1	535133	536101	spr0084	0.0	pathogenitcity related	92.88	98.48	AAK98888
MDDNCMGL_1	1262920	1264401	SPD_0310	0.0	UPF0371 protein SPD_0310	92.11	100.00	ABJ54480
MDDNCMGL_1	765812	768058	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
MDDNCMGL_1	63360	64358	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
MDDNCMGL_1	27245	28216	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
MDDNCMGL_1	1424171	1423020	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
MDDNCMGL_1	1388089	1386101	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
MDDNCMGL_1	1170002	1168272	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.22	100.00	AAK34199
MDDNCMGL_1	1854078	1852600	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
MDDNCMGL_1	622237	623538	Eno	0.0	enolase	86.21	99.77	ABP90468
MDDNCMGL_1	1837892	1836000	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.74	98.90	ABP91129
MDDNCMGL_1	770983	769274	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
MDDNCMGL_1	1694019	1695029	pdh	0.0	pyruvate dehydrogenase	82.79	99.70	ABP91006
MDDNCMGL_1	941342	939981	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
MDDNCMGL_1	607798	608688	bglB	1.34e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
MDDNCMGL_1	850693	849755	SP_0676	2.37e-167	transcription facot	81.15	97.20	AAK74821
MDDNCMGL_1	768250	769107	metF	4.50e-164	methionine biosynthesis enzymes	86.01	99.31	AAK74739
MDDNCMGL_1	1062493	1061789	covR	2.66e-130	responder protein	85.11	100.00	AAK71319
MDDNCMGL_1	1238315	1237644	rr01	3.02e-124	response regulator	83.93	99.56	CAB54566
MDDNCMGL_1	1062493	1061789	walR	1.59e-120	DNA-binding response regulator	81.28	100.00	ANC99504
MDDNCMGL_1	314437	315123	CovR	3.11e-118	serves to repress GAS virulence factor-encoding genes	81.22	100.00	ACE75886
MDDNCMGL_1	774938	775606	CiaR	2.03e-117	two-component response regulator	84.31	99.55	AAK74935
MDDNCMGL_1	774938	775609	ciaR	2.00e-115	two component system response regulator	82.14	99.12	AIK75708
MDDNCMGL_1	64634	65008	SpxA2	1.16e-69	transcriptional regulator	91.20	94.70	ERL21461
MDDNCMGL_1	160053	160553	RopE (M5005_Spy_1611)	4.95e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
MDDNCMGL_1	1541001	1543298	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	83.07	98.08	MYN69560
MDDNCMGL_1	1729880	1728861	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
MDDNCMGL_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found