Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 6 | 0 | 2 | 6.32 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MDLIIIGM_70 | 22886 | 23380 | lnuC | ARO:3002837 | lnuC | 98.17 | 100.00 | AY928180.1 |
| MDLIIIGM_83 | 9398 | 11323 | tet(M) | ARO:3000186 | tet(M) | 98.75 | 100.31 | AB039845.1 |
| MDLIIIGM_107 | 5671 | 6453 | ANT(9)-Ia | ARO:3002630 | ANT(9)-Ia | 100.00 | 100.00 | X02588.1 |
| MDLIIIGM_5 | 729 | 1214 | lnuA | ARO:3002835 | lnuA | 96.89 | 100.00 | M14039.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MDLIIIGM_107 | 5671 | 6453 | ant(9)-Ia | Spectinomycin | 99.87 | 100.00 | X02588 |
| MDLIIIGM_107 | 6579 | 7290 | erm(A) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.86 | 97.27 | X03216 |
| MDLIIIGM_83 | 11518 | 12893 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.49 | 99.93 | AY081910 |
| MDLIIIGM_83 | 9398 | 11323 | tet(M) | Doxycycline, Tetracycline, Minocycline | 99.48 | 100.00 | X75073 |
| MDLIIIGM_70 | 22886 | 23380 | lnu(C) | Lincomycin | 98.99 | 100.00 | AY928180 |
| MDLIIIGM_5 | 729 | 1214 | lnu(A) | Lincomycin | 98.35 | 100.00 | M14039 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MDLIIIGM_107 | 5671 | 6450 | ant(9)-Ia | aminoglycoside nucleotidyltransferase ANT(9)-Ia | EXACTX | 100.00 | 100.00 | WP_000067268.1 |
| MDLIIIGM_107 | 6582 | 7289 | erm(A) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) | BLASTX | 99.58 | 97.12 | WP_001072201.1 |
| MDLIIIGM_5 | 732 | 1214 | lnu(A) | lincosamide nucleotidyltransferase Lnu(A) | BLASTX | 99.38 | 100.00 | WP_001829870.1 |
| MDLIIIGM_83 | 9398 | 11320 | tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | BLASTX | 99.38 | 100.00 | WP_000691727.1 |
| MDLIIIGM_83 | 11517 | 12890 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_002294500.1 |
| MDLIIIGM_70 | 22889 | 23380 | lnu(C) | lincosamide nucleotidyltransferase Lnu(C) | BLASTX | 98.17 | 100.00 | WP_063851341.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MDLIIIGM_80 | 47873 | 47166 | WalR | 1.41e-115 | transcriptional regulatory protein | 85.17 | 100.43 | EPH95667 |
| MDLIIIGM_44 | 30575 | 30378 | CspR | 7.63e-29 | cold shock protein | 81.82 | 100.00 | AAO82613 |
| MDLIIIGM_44 | 30575 | 30381 | CspA | 3.72e-28 | cold shock protein | 81.54 | 98.48 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MDLIIIGM_1 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_3 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_4 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_5 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_6 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_8 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_9 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_10 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_11 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_12 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_13 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_14 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_15 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_17 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_19 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_20 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_21 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_22 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_33 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_44 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_50 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_53 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_54 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_55 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_56 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_57 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_58 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_59 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_60 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_61 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_62 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_63 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_64 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_65 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_66 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_67 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_68 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_69 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_70 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_71 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_72 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_73 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_74 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_75 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_76 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_77 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_78 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_79 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_80 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_81 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_82 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_83 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_84 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_85 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_86 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_87 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_88 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_89 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_90 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_91 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_92 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_93 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_94 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_95 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_96 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_97 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_98 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_99 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_100 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_101 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_102 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_103 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_104 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_105 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_106 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_107 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_108 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_109 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_110 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_111 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_112 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_113 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_114 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_115 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_116 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_117 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_119 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_120 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_121 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_122 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_124 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_125 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_126 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_127 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_128 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_129 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_130 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_131 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_132 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_133 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_134 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_135 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_136 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_137 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_138 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_139 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_140 | 0.0 | 1.0 | 0.0 |
| MDLIIIGM_141 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_143 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_144 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_145 | 1.0 | 0.0 | 1.0 |
| MDLIIIGM_146 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MDLIIIGM_33 | 19099 | 28628 | False | Myoviridae / Siphoviridae | VOG7393, VOG0275, VOG4602, VOG8520, VOG8520, VOG0831, VOG4921, VOG8599, VOG8353, VOG5854, VOG4632, VOG4999 |
| MDLIIIGM_50 | 3349 | 19062 | False | Siphoviridae | VOG8263, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4573, VOG4556, VOG1886, VOG4581, VOG4841, VOG7894, VOG6409 |
| MDLIIIGM_75 | 1697 | 18994 | False | Siphoviridae | VOG0198, VOG1326, VOG4070, VOG1517, VOG4544, VOG0720, VOG4564, VOG4555, VOG1329, VOG4713, VOG0704, VOG0799, VOG0725, VOG0817, VOG6163 |
| MDLIIIGM_102 | 4258 | 29882 | False | Siphoviridae | VOG0321, VOG4548, VOG0322, VOG6545, VOG4632, VOG4609, VOG0327, VOG0198, VOG8681, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG9583, VOG0207, VOG4586, VOG4545, VOG0801, VOG3462, VOG4190 |
| MDLIIIGM_135 | 2129 | 35614 | False | Siphoviridae | VOG8134, VOG4856, VOG7521, VOG4619, VOG0801, VOG5793, VOG0701, VOG9763, VOG0698, VOG0697, VOG0696, VOG0695, VOG4555, VOG5847, VOG0692, VOG0691, VOG4544, VOG3699, VOG8681, VOG0198, VOG0637, VOG0371, VOG7265, VOG4693, VOG7264, VOG4548, VOG5317, VOG5280, VOG7263, VOG0322, VOG0083, VOG10043, VOG1563, VOG11003, VOG8520 |
Phigaro v2.4.0 (Database: Jan. 2024 release)