Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 0 | 2 | 5.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KNDMEGLF_60 | 30769 | 31263 | lnuC | ARO:3002837 | lnuC | 98.17 | 100.00 | AY928180.1 |
| KNDMEGLF_48 | 16348 | 18273 | tet(M) | ARO:3000186 | tet(M) | 98.91 | 100.31 | AB039845.1 |
| KNDMEGLF_48 | 14778 | 16154 | tet(L) | ARO:3000179 | tet(L) | 98.69 | 100.00 | M11036.1 |
| KNDMEGLF_54 | 10932 | 11795 | ANT(6)-Ia | ARO:3002626 | ANT(6)-Ia | 100.00 | 100.00 | KF648874.1 |
| KNDMEGLF_13 | 461 | 946 | lnuA | ARO:3002835 | lnuA | 96.89 | 100.00 | M14039.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KNDMEGLF_54 | 10932 | 11795 | ant(6)-Ia | Streptomycin | 100.00 | 100.00 | KF421157 |
| KNDMEGLF_48 | 14778 | 16153 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.71 | 99.93 | AY081910 |
| KNDMEGLF_48 | 16348 | 18273 | tet(M) | Doxycycline, Tetracycline, Minocycline | 99.48 | 100.00 | X75073 |
| KNDMEGLF_60 | 30769 | 31263 | lnu(C) | Lincomycin | 98.79 | 100.00 | AY928180 |
| KNDMEGLF_13 | 461 | 946 | lnu(A) | Lincomycin | 98.35 | 100.00 | M14039 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KNDMEGLF_54 | 10932 | 11792 | ant(6)-Ia | aminoglycoside nucleotidyltransferase ANT(6)-Ia | EXACTX | 100.00 | 100.00 | WP_002294505.1 |
| KNDMEGLF_48 | 16351 | 18273 | tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | BLASTX | 99.53 | 100.00 | WP_000691727.1 |
| KNDMEGLF_13 | 461 | 943 | lnu(A) | lincosamide nucleotidyltransferase Lnu(A) | BLASTX | 99.38 | 100.00 | WP_001829870.1 |
| KNDMEGLF_48 | 14781 | 16154 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_002294500.1 |
| KNDMEGLF_60 | 30769 | 31260 | lnu(C) | lincosamide nucleotidyltransferase Lnu(C) | BLASTX | 98.17 | 100.00 | WP_063851341.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KNDMEGLF_60 | 55113 | 54406 | WalR | 2.52e-116 | transcriptional regulatory protein | 85.59 | 100.43 | EPH95667 |
| KNDMEGLF_49 | 6027 | 5830 | CspR | 1.27e-28 | cold shock protein | 81.82 | 100.00 | AAO82613 |
| KNDMEGLF_49 | 6027 | 5833 | CspA | 6.20e-28 | cold shock protein | 81.54 | 98.48 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KNDMEGLF_1 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_2 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_3 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_4 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_5 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_6 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_7 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_9 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_10 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_11 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_12 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_13 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_14 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_15 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_16 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_18 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_19 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_20 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_22 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_30 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_31 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_32 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_33 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_34 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_35 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_36 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_37 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_38 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_39 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_40 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_41 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_42 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_43 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_44 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_45 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_46 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_47 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_48 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_49 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_50 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_51 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_52 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_53 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_54 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_55 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_56 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_57 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_58 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_59 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_60 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_61 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_62 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_63 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_64 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_65 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_66 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_67 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_68 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_69 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_70 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_71 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_72 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_73 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_74 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_75 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_76 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_77 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_78 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_79 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_80 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_81 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_82 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_83 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_84 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_85 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_86 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_87 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_88 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_89 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_90 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_91 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_92 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_93 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_94 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_95 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_96 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_97 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_98 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_99 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_100 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_101 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_102 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_103 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_104 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_105 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_106 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_107 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_108 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_109 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_110 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_111 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_112 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_113 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_114 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_115 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_116 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_117 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_118 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_119 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_120 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_122 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_123 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_124 | 1.0 | 0.0 | 1.0 |
| KNDMEGLF_125 | 0.0 | 1.0 | 0.0 |
| KNDMEGLF_126 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KNDMEGLF_36 | 922 | 29317 | False | Siphoviridae | VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG9583, VOG0207, VOG4586, VOG4545, VOG0801, VOG3462, VOG10631, VOG4856, VOG5008, VOG1637, VOG0703, VOG8294, VOG0275, VOG0862 |
| KNDMEGLF_56 | 2369 | 16473 | False | Siphoviridae | VOG9997, VOG4599, VOG0727, VOG4545, VOG0800, VOG0725, VOG0799, VOG0724, VOG5660, VOG0723, VOG4713, VOG4572, VOG10914 |
| KNDMEGLF_93 | 38915 | 43057 | False | Unknown | VOG8353, VOG7894, VOG0831, VOG9667, VOG3699, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)