Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 6 | 0 | 2 | 6.32 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MHCKMFAJ_31 | 23180 | 23674 | lnuC | ARO:3002837 | lnuC | 98.17 | 100.00 | AY928180.1 |
| MHCKMFAJ_74 | 5671 | 6453 | ANT(9)-Ia | ARO:3002630 | ANT(9)-Ia | 100.00 | 100.00 | X02588.1 |
| MHCKMFAJ_47 | 3509 | 5434 | tet(M) | ARO:3000186 | tet(M) | 98.75 | 100.31 | AB039845.1 |
| MHCKMFAJ_101 | 323 | 808 | lnuA | ARO:3002835 | lnuA | 96.89 | 100.00 | M14039.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MHCKMFAJ_74 | 5671 | 6453 | ant(9)-Ia | Spectinomycin | 99.87 | 100.00 | X02588 |
| MHCKMFAJ_74 | 6579 | 7290 | erm(A) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.86 | 97.27 | X03216 |
| MHCKMFAJ_47 | 1939 | 3314 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.49 | 99.93 | AY081910 |
| MHCKMFAJ_47 | 3509 | 5434 | tet(M) | Doxycycline, Tetracycline, Minocycline | 99.48 | 100.00 | X75073 |
| MHCKMFAJ_31 | 23180 | 23674 | lnu(C) | Lincomycin | 98.99 | 100.00 | AY928180 |
| MHCKMFAJ_101 | 323 | 808 | lnu(A) | Lincomycin | 98.35 | 100.00 | M14039 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MHCKMFAJ_74 | 5671 | 6450 | ant(9)-Ia | aminoglycoside nucleotidyltransferase ANT(9)-Ia | EXACTX | 100.00 | 100.00 | WP_000067268.1 |
| MHCKMFAJ_74 | 6582 | 7289 | erm(A) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) | BLASTX | 99.58 | 97.12 | WP_001072201.1 |
| MHCKMFAJ_101 | 323 | 805 | lnu(A) | lincosamide nucleotidyltransferase Lnu(A) | BLASTX | 99.38 | 100.00 | WP_001829870.1 |
| MHCKMFAJ_47 | 3512 | 5434 | tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | BLASTX | 99.38 | 100.00 | WP_000691727.1 |
| MHCKMFAJ_47 | 1942 | 3315 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_002294500.1 |
| MHCKMFAJ_31 | 23183 | 23674 | lnu(C) | lincosamide nucleotidyltransferase Lnu(C) | BLASTX | 98.17 | 100.00 | WP_063851341.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MHCKMFAJ_9 | 20623 | 19916 | WalR | 9.24e-116 | transcriptional regulatory protein | 85.17 | 100.43 | EPH95667 |
| MHCKMFAJ_11 | 30869 | 30672 | CspR | 7.68e-29 | cold shock protein | 81.82 | 100.00 | AAO82613 |
| MHCKMFAJ_11 | 30869 | 30675 | CspA | 3.74e-28 | cold shock protein | 81.54 | 98.48 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MHCKMFAJ_8 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_9 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_10 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_11 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_12 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_13 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_14 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_15 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_16 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_17 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_18 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_19 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_20 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_21 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_22 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_23 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_24 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_25 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_26 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_27 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_28 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_29 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_30 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_31 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_32 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_33 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_34 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_35 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_36 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_37 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_38 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_39 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_40 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_41 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_42 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_43 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_44 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_45 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_46 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_47 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_48 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_49 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_50 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_51 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_52 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_53 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_54 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_55 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_56 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_57 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_58 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_59 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_60 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_61 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_62 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_63 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_64 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_65 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_66 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_67 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_68 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_69 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_70 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_71 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_72 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_73 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_74 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_75 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_76 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_77 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_78 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_79 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_80 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_81 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_82 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_83 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_84 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_85 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_87 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_88 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_89 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_90 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_91 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_92 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_93 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_94 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_95 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_96 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_97 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_98 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_99 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_100 | 0.0 | 1.0 | 0.0 |
| MHCKMFAJ_101 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_102 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_103 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_104 | 1.0 | 0.0 | 1.0 |
| MHCKMFAJ_106 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MHCKMFAJ_10 | 3421 | 19961 | False | Siphoviridae | VOG0801, VOG8263, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4573, VOG4556, VOG1886, VOG4581, VOG4841, VOG7894, VOG6409 |
| MHCKMFAJ_12 | 17072 | 43010 | False | Siphoviridae | VOG4190, VOG3462, VOG0801, VOG4545, VOG4586, VOG0207, VOG9583, VOG0205, VOG0204, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG4841, VOG8681, VOG0198, VOG0327, VOG4609, VOG4632, VOG6545, VOG0322, VOG4548, VOG0321 |
| MHCKMFAJ_35 | 1690 | 18987 | False | Siphoviridae | VOG0198, VOG1326, VOG4070, VOG1517, VOG4544, VOG0720, VOG4564, VOG4555, VOG1329, VOG4713, VOG0704, VOG0799, VOG0725, VOG0817, VOG6163 |
| MHCKMFAJ_84 | 23744 | 57229 | False | Siphoviridae | VOG4602, VOG8520, VOG11003, VOG1563, VOG10043, VOG0083, VOG0322, VOG7263, VOG5280, VOG5317, VOG4548, VOG7264, VOG4693, VOG7265, VOG0371, VOG0637, VOG0198, VOG8681, VOG3699, VOG4544, VOG0691, VOG0692, VOG5847, VOG0129, VOG4555, VOG0695, VOG0696, VOG0697, VOG0698, VOG9763, VOG0701, VOG5793, VOG0801, VOG4619, VOG7521, VOG4856 |
Phigaro v2.4.0 (Database: Jan. 2024 release)